AB75432

Recombinant human MIF protein

Name: Recombinant human MIF protein (ab75432) | Abcam
Brand: Abcam Limited
SKU: AB75432
Price: 215 USD
Availability: InStock

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(1 Publication)

Recombinant human MIF protein is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

GLIF, MMIF, MIF, Macrophage migration inhibitory factor, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase, GIF

1 Images

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Western blot - Recombinant human MIF protein (AB75432)

ab75432 used in Western Blot. Figure : 1 ug run under (+) reducing and (-) non-reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Human MIF has a predicted MW of 12.5 kDa.

All lanes:

Western blot - Recombinant human MIF protein (ab75432)

false

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Active

Accession

P14174

Animal free

Carrier free

Species

Human

Reconstitution

Reconstitute in water

Storage buffer

Constituents: 0.164% Sodium phosphate

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"0-1 µg/mL", "notes":"<p>The activity was measured in an agarose microdroplet assay using human U937 and production of IL-8 by human PBMCs</p>" } } }

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Product details

ab75432 acts as an inflammatory mediator consisting of two alpha-helices and six beta-strands, four of which form a beta-sheet structure. The remaining two beta-strands interact with other MIF molecules forming a trimer in solution. MIF acts on fibroblasts by inducing IL-1, IL-8 and MMP expression. MIF stimulates NO production and TNF-alpha release following IFN-gamma activation on macrophages.

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Sequence info

[{"sequence":"MPMFIVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQLMAFGGSSEPCALCSLHSIGKIGGAQNRSYSKLLCGLLAERLRISPDRVYINYYDMNAANVGWNNSTFA","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P14174","tags":[]}]

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

True

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Macrophage migration inhibitory factor (MIF) also known as glycosylation-inhibiting factor is a protein with a molecular mass of approximately 12.5 kDa. It plays a mechanistic role as a cytokine that regulates immune responses specifically influencing macrophage behavior. MIF expresses in various cell types including immune and epithelial cells. It gets secreted by activated lymphocytes and macrophages pointing to its involvement in the inflammatory process.

Biological function summary

MIF acts as an upstream regulator of innate immunity and exerts broad influence on cytokine release. MIF elevates pro-inflammatory cytokines like TNF-alpha and IL-1beta promoting an immune response. It does not typically form part of a complex but interacts with cell surface receptors like CD74 triggering intracellular signaling pathways. These interactions illustrate MIF as a regulator of immune cell recruitment and activation.

Pathways

MIF participates in the glucocorticoid receptor regulatory pathway and MAP kinase pathway. It shows interaction with JNK and ERK1/2 proteins which further map into important cellular reactions like stress response and cell proliferation. By integrating into these pathways MIF influences cell survival proliferation and inflammatory cascades emphasizing its role in immune regulation.

MIF links to inflammatory diseases such as rheumatoid arthritis and sepsis. It influences disease progression by interacting with proteins like TNF-alpha and IL-6 contributing to inflammatory damage. Elevated MIF levels often correlate with disease severity in rheumatoid arthritis acting as a potential target for therapy. Furthermore sepsis demonstrates a similar pattern where MIF modulation can impact patient outcomes underlining its relevance in clinical investigations.

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Specifications

Form

Lyophilized

Additional notes

Purity is approximately 97% as determined by analysis by RP-HPLC and reducing and non-reducing SDS-PAGE.Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

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General info

Function

Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens (PubMed : 15908412, PubMed : 17443469, PubMed : 23776208). The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense (PubMed : 15908412, PubMed : 17443469, PubMed : 23776208). Counteracts the anti-inflammatory activity of glucocorticoids (PubMed : 15908412, PubMed : 17443469, PubMed : 23776208). Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known (PubMed : 11439086, PubMed : 17526494). It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (PubMed : 11439086, PubMed : 17526494).

Sequence similarities

Belongs to the MIF family.

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information MIF

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 297:101072 PubMed34391782

2021

Identification of invariant chain CD74 as a functional receptor of tissue inhibitor of metalloproteinases-1 (TIMP-1).

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin Schoeps,Celina Eckfeld,Laura Flüter,Selina Keppler,Ritu Mishra,Percy Knolle,Felix Bayerl,Jan Böttcher,Chris D Hermann,Daniel Häußler,Achim Krüger

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Recombinant human MIF protein

Western blot - Recombinant human MIF protein (AB75432)

Key facts

Purity

Expression system

Tags

Applications

Biologically active

Biological activity

Accession

Animal free

Carrier free

Species

Reconstitution

Storage buffer

Reactivity data

Product details

Sequence info

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Specifications

Form

Additional notes

General info

Function

Sequence similarities

Product protocols

Target data

Publications (1)

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