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AB163047

Recombinant Human MIRO1 protein (GST tag N-Terminus)

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(1 Publication)

Recombinant Human MIRO1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 659 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

ARHT1, RHOT1, Mitochondrial Rho GTPase 1, MIRO-1, hMiro-1, Rac-GTP-binding protein-like protein, Ras homolog gene family member T1

1 Images
SDS-PAGE - Recombinant Human MIRO1 protein (GST tag N-Terminus) (AB163047)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MIRO1 protein (GST tag N-Terminus) (AB163047)

ab163047 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

Q8IXI2

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB320019

Anti-MIRO1 antibody [MJF-D29136-145] - BSA and Azide free (Detector)

Recombinant|RabMAb|KO Validated

Sandwich ELISA - Anti-MIRO1 antibody [MJF-D29136-145] - BSA and Azide free (Detector) (AB320019)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB186735

Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735)

  • 5
  • 13
Cell Lines & Lysates

AB265792

Human RHOT1 (MIRO1) knockout HeLa cell line

Advanced Validation

Western blot - Human RHOT1 (MIRO1) knockout HeLa cell line (AB265792)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHISDGVADSGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFKVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAVTVTRDKKIDLQKKQTQRNVFRCNVIGVKNCGKSGVLQALLGRNLMRQKKIREDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAEIICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEYSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPHARLRCMCTCNRCTFCICQNFLNSDLLQSVKNKIFTAVLNRHVTQADLKSSTFWLRASFGATVFAVLGFAMYKALLKQR","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":659,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q8IXI2","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MIRO1 also known as RhoT1 is a mitochondrial Rho GTPase with a molecular mass of approximately 69 kDa. It functions as a pivotal component in mitochondrial transport and dynamics. This protein localizes to the outer mitochondrial membrane and is highly expressed in various tissues particularly in the brain and muscles. MIRO1 facilitates the anchoring of mitochondria to microtubule motors enabling their movement within the cell which is essential for maintaining proper energy distribution.
Biological function summary

MIRO1 plays a significant role in regulating mitochondrial trafficking along the cytoskeleton. It forms part of a protein complex with TRAK1/2 kinesin and dynein motor proteins. MIRO1's presence in this complex allows it to modulate mitochondrial distribution and dynamics which are essential for neural development and function. It also influences mitochondrial shape and interaction with the endoplasmic reticulum impacting intracellular calcium homeostasis.

Pathways

MIRO1 is an integral component of pathways involving mitochondrial transport and cellular energy homeostasis. It actively participates in the regulation of calcium signaling pathways alongside related proteins like TRAK1. Additionally MIRO1's involvement in the maintenance of mitochondrial dynamics links it to mitophagy pathways. These pathways regulate the removal of damaged mitochondria a vital process for cellular health and longevity.

MIRO1 has connections to neurodegenerative conditions such as Parkinson's disease and Alzheimer's disease. These conditions correlate with impaired mitochondrial trafficking and dynamics. MIRO1 interacts with proteins such as PINK1 and Parkin which contribute to mitochondrial quality control and are involved in the pathogenesis of these diseases. The disruption of MIRO1 function can lead to mitochondrial dysfunction and energy deficits which are underlying factors in these neurodegenerative processes.
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Specifications

Form

Liquid

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General info

Function

Atypical mitochondrial nucleoside-triphosphatase (NTPase) involved in mitochondrial trafficking (PubMed : 12482879, PubMed : 16630562, PubMed : 22396657, PubMed : 30513825). Probably involved in control of anterograde transport of mitochondria and their subcellular distribution (PubMed : 12482879, PubMed : 16630562, PubMed : 22396657). Promotes mitochondrial fission during high calcium conditions (PubMed : 27716788). Can hydrolyze GTP, ATP and UTP (PubMed : 30513825).

Sequence similarities

Belongs to the mitochondrial Rho GTPase family.

Post-translational modifications

Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy (PubMed:22396657, PubMed:24896179, PubMed:27605430). Deubiquitinated by USP30 (PubMed:24896179).. Acetylation on Lys-92 decreases sensitivity of mitochondrial transport to elevated Ca(2+) levels, increases mitochondrial transport and promotes axon growth. Deacetylated by HDAC6 which blocks mitochondrial transport and mediates axon growth inhibition.

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Product protocols

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Target data

Atypical mitochondrial nucleoside-triphosphatase (NTPase) involved in mitochondrial trafficking (PubMed : 12482879, PubMed : 16630562, PubMed : 22396657, PubMed : 30513825). Probably involved in control of anterograde transport of mitochondria and their subcellular distribution (PubMed : 12482879, PubMed : 16630562, PubMed : 22396657). Promotes mitochondrial fission during high calcium conditions (PubMed : 27716788). Can hydrolyze GTP, ATP and UTP (PubMed : 30513825).
See full target information RHOT1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell metabolism 30:1131-1140.e7 PubMed31564441

2019

Miro1 Marks Parkinson's Disease Subset and Miro1 Reducer Rescues Neuron Loss in Parkinson's Models.

Applications

Unspecified application

Species

Unspecified reactive species

Chung-Han Hsieh,Li Li,Roeland Vanhauwaert,Kong T Nguyen,Mary D Davis,Guojun Bu,Zbigniew K Wszolek,Xinnan Wang
View all publications
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