Recombinant Human MIRO1 protein is a Human Full Length protein, in the 1 to 659 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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- Cell metabolism 30:1131-1140.e72019Miro1 Marks Parkinson's Disease Subset and Miro1 Reducer Rescues Neuron Loss in Parkinson's Models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChung-Han Hsieh et. al.PubMed 31564441
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M K K D V R I L L V G E P R V G K T S L I M S L V S E E F P E E V P P R A E E I T I P A D V T P E R V P T H I V D Y S E A E Q S D E Q L H Q E I S Q A N V I C I V Y A V N N K H S I D K V T S R W I P L I N E R T D K D S R L P L I L V G N K S D L V E Y S S M E T I L P I M N Q Y T E I E T C V E C S A K N L K N I S E L F Y Y A Q K A V L H P T G P L Y C P E E K E M K P A C I K A L T R I F K I S D Q D N D G T L N D A E L N F F Q R I C F N T P L A P Q A L E D V K N V V R K H I S D G V A D S G L T L K G F L F L H T L F I Q R G R H E T T W T V L R R F G Y D D D L D L T P E Y L F P L L K I P P D C T T E L N H H A Y L F L Q S T F D K H D L D R D C A L S P D E L K D L F K V F P Y I P W G P D V N N T V C T N E R G W I T Y Q G F L S Q W T L T T Y L D V Q R C L E Y L G Y L G Y S I L T E Q E S Q A S A V T V T R D K K I D L Q K K Q T Q R N V F R C N V I G V K N C G K S G V L Q A L L G R N L M R Q K K I R E D H K S Y Y A I N T V Y V Y G Q E K Y L L L H D I S E S E F L T E A E I I C D V V C L V Y D V S N P K S F E Y C A R I F K Q H F M D S R I P C L I V A A K S D L H E V K Q E Y S I S P T D F C R K H K M P P P Q A F T C N T A D A P S K D I F V K L T T M A M Y P H A R L R C M C T C N R C T F C I C Q N F L N S D L L Q S V K N K I F T A V L N R H V T Q A D L K S S T F W L R A S F G A T V F A V L G F A M Y K A L L K Q R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Mitochondrial GTPase involved in mitochondrial trafficking (PubMed:12482879, PubMed:16630562, PubMed:22396657). Probably involved in control of anterograde transport of mitochondria and their subcellular distribution (PubMed:12482879, PubMed:16630562, PubMed:22396657). Promotes mitochondrial fission during high calcium conditions (PubMed:27716788).
Alternative names
ARHT1, RHOT1, Mitochondrial Rho GTPase 1, MIRO-1, hMiro-1, Rac-GTP-binding protein-like protein, Ras homolog gene family member T1
Recommended products
Recombinant Human MIRO1 protein is a Human Full Length protein, in the 1 to 659 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q8IXI2-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M K K D V R I L L V G E P R V G K T S L I M S L V S E E F P E E V P P R A E E I T I P A D V T P E R V P T H I V D Y S E A E Q S D E Q L H Q E I S Q A N V I C I V Y A V N N K H S I D K V T S R W I P L I N E R T D K D S R L P L I L V G N K S D L V E Y S S M E T I L P I M N Q Y T E I E T C V E C S A K N L K N I S E L F Y Y A Q K A V L H P T G P L Y C P E E K E M K P A C I K A L T R I F K I S D Q D N D G T L N D A E L N F F Q R I C F N T P L A P Q A L E D V K N V V R K H I S D G V A D S G L T L K G F L F L H T L F I Q R G R H E T T W T V L R R F G Y D D D L D L T P E Y L F P L L K I P P D C T T E L N H H A Y L F L Q S T F D K H D L D R D C A L S P D E L K D L F K V F P Y I P W G P D V N N T V C T N E R G W I T Y Q G F L S Q W T L T T Y L D V Q R C L E Y L G Y L G Y S I L T E Q E S Q A S A V T V T R D K K I D L Q K K Q T Q R N V F R C N V I G V K N C G K S G V L Q A L L G R N L M R Q K K I R E D H K S Y Y A I N T V Y V Y G Q E K Y L L L H D I S E S E F L T E A E I I C D V V C L V Y D V S N P K S F E Y C A R I F K Q H F M D S R I P C L I V A A K S D L H E V K Q E Y S I S P T D F C R K H K M P P P Q A F T C N T A D A P S K D I F V K L T T M A M Y P H A R L R C M C T C N R C T F C I C Q N F L N S D L L Q S V K N K I F T A V L N R H V T Q A D L K S S T F W L R A S F G A T V F A V L G F A M Y K A L L K Q R
- Accession
- Q8IXI2
- Protein length
- Full Length
- Amino acids
- 1 to 659
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Mitochondrial GTPase involved in mitochondrial trafficking (PubMed:12482879, PubMed:16630562, PubMed:22396657). Probably involved in control of anterograde transport of mitochondria and their subcellular distribution (PubMed:12482879, PubMed:16630562, PubMed:22396657). Promotes mitochondrial fission during high calcium conditions (PubMed:27716788).
- Sequence similarities
Belongs to the mitochondrial Rho GTPase family.
- Post-translational modifications
Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy (PubMed:22396657, PubMed:24896179). Deubiquitinated by USP30 (PubMed:24896179).
- Subcellular localisation
- Mitochondrion outer membrane
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MIRO1 also known as RhoT1 is a mitochondrial Rho GTPase with a molecular mass of approximately 69 kDa. It functions as a pivotal component in mitochondrial transport and dynamics. This protein localizes to the outer mitochondrial membrane and is highly expressed in various tissues particularly in the brain and muscles. MIRO1 facilitates the anchoring of mitochondria to microtubule motors enabling their movement within the cell which is essential for maintaining proper energy distribution.
- Biological function summary
MIRO1 plays a significant role in regulating mitochondrial trafficking along the cytoskeleton. It forms part of a protein complex with TRAK1/2 kinesin and dynein motor proteins. MIRO1's presence in this complex allows it to modulate mitochondrial distribution and dynamics which are essential for neural development and function. It also influences mitochondrial shape and interaction with the endoplasmic reticulum impacting intracellular calcium homeostasis.
- Pathways
MIRO1 is an integral component of pathways involving mitochondrial transport and cellular energy homeostasis. It actively participates in the regulation of calcium signaling pathways alongside related proteins like TRAK1. Additionally MIRO1's involvement in the maintenance of mitochondrial dynamics links it to mitophagy pathways. These pathways regulate the removal of damaged mitochondria a vital process for cellular health and longevity.
- Associated diseases and disorders
MIRO1 has connections to neurodegenerative conditions such as Parkinson's disease and Alzheimer's disease. These conditions correlate with impaired mitochondrial trafficking and dynamics. MIRO1 interacts with proteins such as PINK1 and Parkin which contribute to mitochondrial quality control and are involved in the pathogenesis of these diseases. The disruption of MIRO1 function can lead to mitochondrial dysfunction and energy deficits which are underlying factors in these neurodegenerative processes.
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1 product image
SDS-PAGE - Recombinant Human MIRO1 protein (ab163047)
ab163047 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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