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AB139793

Recombinant Human MIS12 protein (denatured)

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Recombinant Human MIS12 protein (denatured) is a Human Full Length protein, in the 1 to 205 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

Protein MIS12 homolog, MIS12

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SDS-PAGE - Recombinant Human MIS12 protein (denatured) (AB139793)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MIS12 protein (denatured) (AB139793)

15% SDS-PAGE analysis of ab139793 (3μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9H081

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 12.01% Urea, 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMSVDPMTYEAQFFGFTPQTCMLRIYIAFQDYLFEVMQAVEQVILKKLDGIPDCDISPVQIRKCTEKFLCFMKGHFDNLFSKMEQLFLQLILRIPSNILLPEDKCKETPYSEEDFQHLQKEIEQLQEKYKTELCTKQALLAELEEQKIVQAKLKQTLTFFDELHNVGRDHGTSDFRESLVSLVQNSRKLQNIRDNVEKESKRLKIS","proteinLength":"Full Length","predictedMolecularWeight":"26.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":205,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9H081","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MIS12 also known as MIND kinetochore complex component is a protein primarily involved in chromosome segregation during cell division. The human MIS12 protein has a mass of approximately 22 kDa. It is expressed in proliferating cells especially during mitosis and is a cytoplasmic protein that localizes to the kinetochore an important structure on chromosomes. MIS12 plays a direct role in the stabilization of spindle microtubules to kinetochores ensuring proper chromosome alignment and segregation.
Biological function summary

This protein functions as part of the MIS12 complex which is essential for accurate cytokinesis. The complex is a critical part of the kinetochore-microtubule attachments necessary for mitotic spindle assembly and chromosome movement. MIS12 also interacts with other kinetochore proteins such as NDC80 and DSN1 forming a network that acts in the early mitotic phase of the cell cycle.

Pathways

MIS12 participates in the Spindle Assembly Checkpoint (SAC) a regulatory mechanism ensuring error-free chromosome segregation. It integrates into the mitotic checkpoint signaling pathway interacting with proteins like BUB1 and MAD2 which monitor attachment and tension at kinetochores. This interaction halts progression through mitosis if incorrect attachments are detected preventing chromosomal instability.

Disruptions in the MIS12 protein function are linked to cancers particularly those exhibiting chromosomal instability such as colorectal cancer. MIS12 mutations or misexpression can cause defective chromosome segregation contributing to aneuploidy and tumorigenesis. Additionally its interaction with the APC/C complex important for cell cycle exit connects it to E2F deregulation in cancerous cells.
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Specifications

Form

Liquid

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General info

Function

Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis (PubMed : 12515822, PubMed : 15502821, PubMed : 16585270). Essential for proper kinetochore microtubule attachments (PubMed : 23891108).

Sequence similarities

Belongs to the mis12 family.

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Product protocols

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Target data

Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis (PubMed : 12515822, PubMed : 15502821, PubMed : 16585270). Essential for proper kinetochore microtubule attachments (PubMed : 23891108).
See full target information MIS12
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Complementary Products

Primary Antibodies

AB70843

Anti-MIS12 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIS12 antibody (AB70843)

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