Recombinant Human MiTF protein is a Human Full Length protein, in the 1 to 520 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M Q S E S G I V P D F E V G E E F H E E P K T Y Y E L K S Q P L K S S S S A E H P G A S K P P I S S S S M T S R I L L R Q Q L M R E Q M Q E Q E R R E Q Q Q K L Q A A Q F M Q Q R V P V S Q T P A I N V S V P T T L P S A T Q V P M E V L K V Q T H L E N P T K Y H I Q Q A Q R Q Q V K Q Y L S T T L A N K H A N Q V L S L P C P N Q P G D H V M P P V P G S S A P N S P M A M L T L N S N C E K E G F Y K F E E Q N R A E S E C P G M N T H S R A S C M Q M D D V I D D I I S L E S S Y N E E I L G L M D P A L Q M A N T L P V S G N L I D L Y G N Q G L P P P G L T I S N S C P A N L P N I K R E L T E S E A R A L A K E R Q K K D N H N L I E R R R R F N I N D R I K E L G T L I P K S N D P D M R W N K G T I L K A S V D Y I R K L Q R E Q Q R A K E L E N R Q K K L E H A N R H L L L R I Q E L E M Q A R A H G L S L I P S T G L C S P D L V N R I I K Q E P V L E N C S Q D L L Q H H A D L T C T T T L D L T D G T I T F N N N L G T G T E A N Q A Y S V P T K M G S K L E D I L M D D T L S P V G V T D P L L S S V S P G A S K T S S R R S S M S M E E T E H T C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Transcription factor that acts as a master regulator of melanocyte survival and differentiation as well as melanosome biogenesis (PubMed:10587587, PubMed:22647378, PubMed:27889061, PubMed:9647758). Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoter of pigmentation genes, such as tyrosinase (TYR) (PubMed:10587587, PubMed:22647378, PubMed:27889061, PubMed:9647758). Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, MITF phosphorylation by MTOR promotes its inactivation (PubMed:36608670). Upon starvation or lysosomal stress, inhibition of MTOR induces MITF dephosphorylation, resulting in transcription factor activity (PubMed:36608670). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1) (PubMed:10587587, PubMed:22647378, PubMed:27889061, PubMed:9647758). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium (PubMed:10587587, PubMed:22647378, PubMed:27889061, PubMed:9647758).
Alternative names
BHLHE32, MITF, Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32
Recommended products
Recombinant Human MiTF protein is a Human Full Length protein, in the 1 to 520 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- O75030-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M Q S E S G I V P D F E V G E E F H E E P K T Y Y E L K S Q P L K S S S S A E H P G A S K P P I S S S S M T S R I L L R Q Q L M R E Q M Q E Q E R R E Q Q Q K L Q A A Q F M Q Q R V P V S Q T P A I N V S V P T T L P S A T Q V P M E V L K V Q T H L E N P T K Y H I Q Q A Q R Q Q V K Q Y L S T T L A N K H A N Q V L S L P C P N Q P G D H V M P P V P G S S A P N S P M A M L T L N S N C E K E G F Y K F E E Q N R A E S E C P G M N T H S R A S C M Q M D D V I D D I I S L E S S Y N E E I L G L M D P A L Q M A N T L P V S G N L I D L Y G N Q G L P P P G L T I S N S C P A N L P N I K R E L T E S E A R A L A K E R Q K K D N H N L I E R R R R F N I N D R I K E L G T L I P K S N D P D M R W N K G T I L K A S V D Y I R K L Q R E Q Q R A K E L E N R Q K K L E H A N R H L L L R I Q E L E M Q A R A H G L S L I P S T G L C S P D L V N R I I K Q E P V L E N C S Q D L L Q H H A D L T C T T T L D L T D G T I T F N N N L G T G T E A N Q A Y S V P T K M G S K L E D I L M D D T L S P V G V T D P L L S S V S P G A S K T S S R R S S M S M E E T E H T C
- Accession
- O75030
- Protein length
- Full Length
- Amino acids
- 1 to 520
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Transcription factor that acts as a master regulator of melanocyte survival and differentiation as well as melanosome biogenesis (PubMed:10587587, PubMed:22647378, PubMed:27889061, PubMed:9647758). Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoter of pigmentation genes, such as tyrosinase (TYR) (PubMed:10587587, PubMed:22647378, PubMed:27889061, PubMed:9647758). Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, MITF phosphorylation by MTOR promotes its inactivation (PubMed:36608670). Upon starvation or lysosomal stress, inhibition of MTOR induces MITF dephosphorylation, resulting in transcription factor activity (PubMed:36608670). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1) (PubMed:10587587, PubMed:22647378, PubMed:27889061, PubMed:9647758). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium (PubMed:10587587, PubMed:22647378, PubMed:27889061, PubMed:9647758).
- Sequence similarities
Belongs to the MiT/TFE family.
- Post-translational modifications
When nutrients are present, phosphorylation by MTOR at Ser-5 via non-canonical mTORC1 pathway promotes ubiquitination by the SCF(BTRC) complex, followed by degradation (PubMed:36608670). Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter (PubMed:10587587). Phosphorylation by MARK3/cTAK1 at Ser-280 promotes association with 14-3-3/YWHA adapters and retention in the cytosol (PubMed:16822840). Phosphorylated at Ser-180 and Ser-516 following KIT signaling, triggering a short live activation: Phosphorylation at Ser-180 and Ser-516 by MAPK and RPS6KA1, respectively, activate the transcription factor activity but also promote ubiquitination and subsequent degradation by the proteasome (PubMed:10673502). Phosphorylated in response to blue light (415nm) (PubMed:28842328).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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1 product image
SDS-PAGE - Recombinant Human MiTF protein (ab158898)
ab158898 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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