Recombinant Human MK-3 protein
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Recombinant Human MK-3 protein is a Human Full Length protein, expressed in Escherichia coli, with >90%.
View Alternative Names
MAP kinase-activated protein kinase 3, MAPK-activated protein kinase 3, MAPKAP kinase 3, MAPKAP-K3, MAPKAPK-3, MK-3, Chromosome 3p kinase, 3pK, MAPKAPK3
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human MK-3 protein (AB45155)
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MK-3 functions as part of the MAPK signaling cascade and phospho-regulates different substrates that influence cell differentiation and stress responses. The protein interacts within complexes that mediate cellular responses to growth factors and various extracellular signals. MK-3's enzymatic activity modulates pathways involved in inflammation and apoptosis which are essential for maintaining cellular homeostasis and responding to stress.
Pathways
MK-3 plays a significant role in the MAPK and SAPK/JNK pathways. These pathways help regulate responses to environmental stressors and cytokine signaling. MK-3 is closely related to other kinases like p38 MAPK and JNK which collaboratively modulate cellular stress responses. These pathways are important for facilitating communication between extracellular stimuli and intracellular actions.
Specifications
Form
Liquid
Additional notes
Purified via GST Tag
General info
Function
Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally : acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells : required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression.
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Post-translational modifications
Phosphorylated and activated by MAPK1/ERK2 and MAPK3/ERK1. Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-201, Ser-251 and Thr-313 (By similarity).
Subcellular localisation
Nucleus
Target data
Complementary Products
Product promise
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