Recombinant Human MK2 protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, WB, FuncS.
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Key facts
- Purity
- >70% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab79910 can be used as a WB positive control in conjunction with ab63574. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling |
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Target data
Function
Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, CEP131, ELAVL1, HNRNPA0, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Phosphorylates HSF1; leading to the interaction with HSP90 proteins and inhibiting HSF1 homotrimerization, DNA-binding and transactivation activities (PubMed:16278218). Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to the dissociation of HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impairment of their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to the regulation of the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity, leading to inhibition of dependent degradation of ARE-containing transcripts. Phosphorylates CEP131 in response to cellular stress induced by ultraviolet irradiation which promotes binding of CEP131 to 14-3-3 proteins and inhibits formation of novel centriolar satellites (PubMed:26616734). Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilization of GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.
Alternative names
MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase 2, MAPKAP-K2, MAPKAPK-2, MK-2, MK2, MAPKAPK2
Recommended products
Recombinant Human MK2 protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >70% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- No
- Accession
- P49137-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 50% Glycerol (glycerin, glycerine), 0.8004% Sodium chloride, 0.395% Tris HCl, 0.05% Sorbitan monolaurate, ethoxylated, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- Accession
- P49137
- Protein length
- Full Length
- Predicted molecular weight
- 72 kDa
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, CEP131, ELAVL1, HNRNPA0, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Phosphorylates HSF1; leading to the interaction with HSP90 proteins and inhibiting HSF1 homotrimerization, DNA-binding and transactivation activities (PubMed:16278218). Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to the dissociation of HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impairment of their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to the regulation of the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity, leading to inhibition of dependent degradation of ARE-containing transcripts. Phosphorylates CEP131 in response to cellular stress induced by ultraviolet irradiation which promotes binding of CEP131 to 14-3-3 proteins and inhibits formation of novel centriolar satellites (PubMed:26616734). Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilization of GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.
- Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
- Post-translational modifications
Sumoylation inhibits the protein kinase activity.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as MAPKAP Kinase 2
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MK2 protein also known as mitogen-activated protein kinase-activated protein kinase 2 or MAPKAPK2 is an important enzyme involved in cellular signaling. MK2 has a protein mass of approximately 44 kDa. It is widely expressed in various tissues indicating its broad functional roles. MK2 interacts with MAP kinases facilitating the transmission of signals within cells during stress responses and inflammation.
- Biological function summary
MK2 plays an important role in the cellular stress response by regulating cytokine production particularly in inflammation. It acts as part of a protein complex with p38 MAPK enhancing its kinase activity. This interaction allows MK2 to control the expression of inflammatory cytokines and other stress-responsive proteins. The complex also responds to cellular stress signals allowing it to modulate gene expression effectively.
- Pathways
MK2 contributes to the p38 MAPK signaling pathway a major route for stress and inflammatory responses in cells. MK2 often interacts with related proteins like p38 MAPK which are directly upstream in the signaling cascade. Additionally MK2 is involved in the post-transcriptional regulation of mRNA stability and translation interfacing with the NF-kB signaling pathway highlighting its regulatory role in inflammation.
- Associated diseases and disorders
MK2's role in inflammation links it to numerous inflammatory diseases such as rheumatoid arthritis and Crohn's disease. MK2's interaction with other proteins like p38 MAPK and downstream effectors such as transcription factors emphasizes its therapeutic potential. Targeting MK2 and its interaction with these proteins may offer novel treatments for conditions characterized by dysregulated inflammatory responses.
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2 product images
SDS-PAGE - Recombinant Human MK2 protein (ab79910)
Lane 1: ab79910 on 10% SDS-PAGE, Coomassie staining, 7μg.
Lane 2: Protein marker.
Western blot - Recombinant Human MK2 protein (ab79910)
All lanes: Anti-MAPKAP Kinase 2 antibody (ab63574) at 1/500 dilution
Lane 1: Western blot - Recombinant Human MK2 protein (ab79910) at 0.01 µg
Lane 2: Western blot - Recombinant Human MK2 protein (ab79910) at 0.001 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 90s
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