Recombinant Human MKK7 protein is a Human Fragment protein, in the 1 to 99 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M A A S S L E Q K L S R L E A K L K Q E N R E A R R R I D L N L D I S P Q R P R P T L Q L P L A N D G G S R S P S S E S S P Q H P T P P A R P R H M L G L P S T L F T P R S M E S I E I D Q K L Q E I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by pro-inflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Part of a non-canonical MAPK signaling pathway, composed of the upstream MAP3K12 kinase and downstream MAP kinases MAPK1/ERK2 and MAPK3/ERK1, that enhances the AP-1-mediated transcription of APP in response to APOE (PubMed:28111074).
Alternative names
JNKK2, MEK7, MKK7, PRKMK7, SKK4, MAP2K7, Dual specificity mitogen-activated protein kinase kinase 7, MAP kinase kinase 7, MAPKK 7, JNK-activating kinase 2, MAPK/ERK kinase 7, Stress-activated protein kinase kinase 4, c-Jun N-terminal kinase kinase 2, MEK 7, SAPK kinase 4, SAPKK-4, SAPKK4, JNK kinase 2, JNKK 2
Recommended products
Recombinant Human MKK7 protein is a Human Fragment protein, in the 1 to 99 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- O14733-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A A S S L E Q K L S R L E A K L K Q E N R E A R R R I D L N L D I S P Q R P R P T L Q L P L A N D G G S R S P S S E S S P Q H P T P P A R P R H M L G L P S T L F T P R S M E S I E I D Q K L Q E I
- Accession
- O14733
- Protein length
- Fragment
- Amino acids
- 1 to 99
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by pro-inflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Part of a non-canonical MAPK signaling pathway, composed of the upstream MAP3K12 kinase and downstream MAP kinases MAPK1/ERK2 and MAPK3/ERK1, that enhances the AP-1-mediated transcription of APP in response to APOE (PubMed:28111074).
- Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
- Post-translational modifications
Activated by phosphorylation on Ser-271 and Thr-275 by MAP kinase kinase kinases (MAP3Ks).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as MEK7.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MKK7 also known as MAP2K7 or Mitogen-activated protein kinase kinase 7 is a dual-specificity kinase involved in the phosphorylation of target proteins. It functions as a critical member of the MAP2K family with a molecular mass of approximately 44 kDa. MKK7 is widely expressed in various tissues including the brain and immune cells which indicates its diverse functional roles across different biological systems.
- Biological function summary
MKK7 plays an essential role in the activation of the JNK (c-Jun N-terminal kinases) signaling pathway by phosphorylating and activating JNK. It operates as part of a signaling complex that responds to stress stimuli. This kinase is involved in numerous cellular processes such as apoptosis differentiation and inflammatory responses. The regulation of these processes by MKK7 impacts many aspects of cellular homeostasis and response to environmental changes.
- Pathways
MKK7 is integrally involved in the stress-activated MAPK pathway and the cellular response to inflammatory cytokines. It closely collaborates with related proteins such as JNK1 JNK2 and JNK3 to mediate its downstream effects. Activation of this pathway leads to the modulation of gene expression that governs cell survival proliferation and other critical cellular functions.
- Associated diseases and disorders
Dysregulation of MKK7 activity connects the protein to disorders like cancer and neurodegenerative diseases. Altered MKK7-JNK signaling contributes to the pathogenesis of certain cancers by affecting cell proliferation and survival mechanisms. In neurodegenerative diseases MKK7-mediated pathways are associated with neuronal stress responses and cell death highlighting its interaction with proteins like Amyloid-beta in Alzheimer's disease. Understanding MKK7's role in these conditions offers potential for therapeutic interventions.
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1 product image
SDS-PAGE - Recombinant Human MKK7 protein (ab159223)
ab159223 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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