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AB114331

Recombinant Human MKP-1 protein

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(1 Publication)

Recombinant Human MKP-1 protein is a Human Full Length protein, in the 1 to 367 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

CL100, MKP1, PTPN10, VH1, DUSP1, Dual specificity protein phosphatase 1, Dual specificity protein phosphatase hVH1, Mitogen-activated protein kinase phosphatase 1, Protein-tyrosine phosphatase CL100, MAP kinase phosphatase 1, MKP-1

1 Images
SDS-PAGE - Recombinant Human MKP-1 protein (AB114331)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MKP-1 protein (AB114331)

ab114331 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

ELISA, WB, SDS-PAGE

applications

Biologically active

No

Accession

P28562

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MVMEVGTLDAGGLRALLGERAAQCLLLDCRSFFAFNAGHIAGSVNVRFSTIVRRRAKGAMGLEHIVPNAELRGRLLAGAYHAVVLLDERSAALDGAKRDGTLALAAGALCREARAAQVFFLKGGYEAFSASCPELCSKQSTPMGLSLPLSTSVPDSAESGCSSCSTPLYDQGGPVEILPFLYLGSAYHASRKDMLDALGITALINVSANCPNHFEGHYQYKSIPVEDNHKADISSWFNEAIDFIDSIKNAGGRVFVHCQAGISRSATICLAYLMRTNRVKLDEAFEFVKQRRSIISPNFSFMGQLLQFESQVLAPHCSAEAGSPAMAVLDRGTSTTTVFNFPVSIPVHSTNSALSYLQSPITTSPSC","proteinLength":"Full Length","predictedMolecularWeight":"66.44 kDa","actualMolecularWeight":null,"aminoAcidEnd":367,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P28562","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as MKP-1 also called DUSP1 is a dual-specificity phosphatase. It acts mechanically by dephosphorylating the phospho-tyrosine and phospho-serine/threonine residues of its target proteins. The protein has a molecular weight of about 39 kDa. MKP-1 is ubiquitously expressed but shows higher levels in specific tissues such as liver lung and brain where it regulates various cellular processes.
Biological function summary

MKP-1 influences cellular responses to stress and inflammation by inactivating mitogen-activated protein kinases (MAPKs). It does not exist as part of a permanent complex but temporarily interacts with MAPKs. By dephosphorylating these kinases MKP-1 modulates gene expression cell proliferation and apoptosis. Through these interactions the protein plays a significant role in protecting cells from excessive inflammatory signals and stress-induced damage.

Pathways

The MKP-1 protein is integral to the MAPK signaling pathway and the JNK signaling cascade. MKP-1 directly affects the balance of MAPK-related proteins such as ERK JNK and p38 to help regulate various cellular outcomes. By dephosphorylating these proteins MKP-1 acts as a negative feedback regulator ensuring that cellular responses to external stimuli are controlled and do not become harmful.

MKP-1 is associated with inflammatory diseases and cancer. Inflammatory disorders such as rheumatoid arthritis show link with aberrant regulation of MKP-1 as it often interacts with MAPKs involved in inflammation. In cancer altered MKP-1 expression can affect tumor growth and progression due to its regulatory role on cell proliferation pathways. The protein's interaction with key signaling molecules in these diseases highlights its potential as a therapeutic target.
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Specifications

Form

Liquid

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General info

Function

Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

Post-translational modifications

Phosphorylation at Ser-359 and Ser-364 by MAPK1/ERK2 and MAPK3/ERK1 reduces its rate of degradation.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.
See full target information DUSP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

EMBO molecular medicine 13:e12461 PubMed33665961

2021

Combination therapies induce cancer cell death through the integrated stress response and disturbed pyrimidine metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Goetz Hartleben,Kenji Schorpp,Yun Kwon,Barbara Betz,Foivos-Filippos Tsokanos,Zahra Dantes,Arlett Schäfer,Ina Rothenaigner,José Manuel Monroy Kuhn,Pauline Morigny,Lisa Mehr,Sean Lin,Susanne Seitz,Janina Tokarz,Anna Artati,Jerzy Adamsky,Oliver Plettenburg,Dominik Lutter,Martin Irmler,Johannes Beckers,Maximilian Reichert,Kamyar Hadian,Anja Zeigerer,Stephan Herzig,Mauricio Berriel Diaz
View all publications
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Complementary Products

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Product promise

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