Recombinant Human MLC1SA protein is a Human Full Length protein, in the 1 to 208 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M P P K K D V P V K K P A G P S I S K P A A K P A A A G A P P A K T K A E P A V P Q A P Q K T Q E P P V D L S K V V I E F N K D Q L E E F K E A F E L F D R V G D G K I L Y S Q C G D V M R A L G Q N P T N A E V L K V L G N P K S D E L K S R R V D F E T F L P M L Q A V A K N R G Q G T Y E D Y L E G F R V F D K E G N G K V M G A E L R H V L T T L G E K M T E E E V E T V L A G H E D S N G C I N Y E A F L K H I L S V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Regulatory light chain of myosin. Does not bind calcium.
Alternative names
MLC1SA, MYL6B, Myosin light chain 6B, Myosin light chain 1 slow-twitch muscle A isoform, Smooth muscle and nonmuscle myosin light chain alkali 6B, MLC1sa
Recommended products
Recombinant Human MLC1SA protein is a Human Full Length protein, in the 1 to 208 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P14649-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris-HCl buffer, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M P P K K D V P V K K P A G P S I S K P A A K P A A A G A P P A K T K A E P A V P Q A P Q K T Q E P P V D L S K V V I E F N K D Q L E E F K E A F E L F D R V G D G K I L Y S Q C G D V M R A L G Q N P T N A E V L K V L G N P K S D E L K S R R V D F E T F L P M L Q A V A K N R G Q G T Y E D Y L E G F R V F D K E G N G K V M G A E L R H V L T T L G E K M T E E E V E T V L A G H E D S N G C I N Y E A F L K H I L S V
- Accession
- P14649
- Protein length
- Full Length
- Predicted molecular weight
- 25.2 kDa
- Amino acids
- 1 to 208
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab111637 was purified using conventional chromatography techniques.
General info
- Function
Regulatory light chain of myosin. Does not bind calcium.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MLC1SA also known as myosin light chain 1 slow-twitch muscle isoform plays a significant role in muscle contraction. It binds to myosin heavy chains in muscle fibers regulating the actin-myosin interaction essential for muscle contraction. MLC1SA has a molecular mass of approximately 18 kDa and shows expression predominantly in slow-twitch muscle fibers which are important for endurance activities. It also exhibits expression in certain cardiac tissues highlighting its function beyond skeletal muscles.
- Biological function summary
MLC1SA contributes to muscle contraction regulation by modulating the interaction between actin and myosin. It is part of a complex that includes myosin heavy chains and other associated proteins. Through phosphorylation events MLC1SA adjusts the sensitivity of muscle fibers to calcium which is critical for the fine-tuning of muscle responses to stimuli. This subtle modulation is important for activities that rely on sustained muscle tension.
- Pathways
Myosin light chain signaling involves MLC1SA as a central component in muscular contraction pathways. It plays a role in the myosin-actin crossbridge cycle aiding the efficient conversion of ATP into mechanical work. MLC1SA interacts with other proteins such as myosin heavy chains and calmodulin. These interactions are critical in pathways like the calcium signaling pathway and muscle contraction pathway where calcium ions serve as triggers for changes in muscle cell activity.
- Associated diseases and disorders
Defects or alterations in MLC1SA expression or function can relate to muscular dystrophies and myopathies. These conditions often involve muscle weakness and reduced endurance where MLC1SA disruption alters normal muscle function. Moreover cardiomyopathies can show links with this protein especially when considering the interaction with myosin heavy chains and tropomyosin. Alterations in these interactions may disrupt cardiac muscle contraction contributing to disease pathogenesis.
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1 product image
SDS-PAGE - Recombinant Human MLC1SA protein (ab111637)
15% SDS-PAGE analysis of ab111637 (3 μg)
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