Recombinant Human MLH1 protein is a Human Full Length protein, in the 1 to 756 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M S F V A G V I R R L D E T V V N R I A A G E V I Q R P A N A I K E M I E N C L D A K S T S I Q V I V K E G G L K L I Q I Q D N G T G I R K E D L D I V C E R F T T S K L Q S F E D L A S I S T Y G F R G E A L A S I S H V A H V T I T T K T A D G K C A Y R A S Y S D G K L K A P P K P C A G N Q G T Q I T V E D L F Y N I A T R R K A L K N P S E E Y G K I L E V V G R Y S V H N A G I S F S V K K Q G E T V A D V R T L P N A S T V D N I R S I F G N A V S R E L I E I G C E D K T L A F K M N G Y I S N A N Y S V K K C I F L L F I N H R L V E S T S L R K A I E T V Y A A Y L P K N T H P F L Y L S L E I S P Q N V D V N V H P T K H E V H F L H E E S I L E R V Q Q H I E S K L L G S N S S R M Y F T Q T L L P G L A G P S G E M V K S T T S L T S S S T S G S S D K V Y A H Q M V R T D S R E Q K L D A F L Q P L S K P L S S Q P Q A I V T E D K T D I S S G R A R Q Q D E E M L E L P A P A E V A A K N Q S L E G D T T K G T S E M S E K R G P T S S N P R K R H R E D S D V E M V E D D S R K E M T A A C T P R R R I I N L T S V L S L Q E E I N E Q G H E V L R E M L H N H S F V G C V N P Q W A L A Q H Q T K L Y L L N T T K L S E E L F Y Q I L I Y D F A N F G V L R L S E P A P L F D L A M L A L D S P E S G W T E E D G P K E G L A E Y I V E F L K K K A E M L A D Y F S L E I D E E G N L I G L P L L I D N Y V P P L E G L P I F I L R L A T E V N W D E E K E C F E S L S K E C A M F Y S I R K Q Y I S E E S T L S G Q Q S E V P G S I P N S W K W T V E H I V Y K A L R S H I L P P K H F T E D G N I L Q L A N L P D L Y K V F E R C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
Alternative names
COCA2, MLH1, DNA mismatch repair protein Mlh1, MutL protein homolog 1
Recommended products
Recombinant Human MLH1 protein is a Human Full Length protein, in the 1 to 756 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P40692-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M S F V A G V I R R L D E T V V N R I A A G E V I Q R P A N A I K E M I E N C L D A K S T S I Q V I V K E G G L K L I Q I Q D N G T G I R K E D L D I V C E R F T T S K L Q S F E D L A S I S T Y G F R G E A L A S I S H V A H V T I T T K T A D G K C A Y R A S Y S D G K L K A P P K P C A G N Q G T Q I T V E D L F Y N I A T R R K A L K N P S E E Y G K I L E V V G R Y S V H N A G I S F S V K K Q G E T V A D V R T L P N A S T V D N I R S I F G N A V S R E L I E I G C E D K T L A F K M N G Y I S N A N Y S V K K C I F L L F I N H R L V E S T S L R K A I E T V Y A A Y L P K N T H P F L Y L S L E I S P Q N V D V N V H P T K H E V H F L H E E S I L E R V Q Q H I E S K L L G S N S S R M Y F T Q T L L P G L A G P S G E M V K S T T S L T S S S T S G S S D K V Y A H Q M V R T D S R E Q K L D A F L Q P L S K P L S S Q P Q A I V T E D K T D I S S G R A R Q Q D E E M L E L P A P A E V A A K N Q S L E G D T T K G T S E M S E K R G P T S S N P R K R H R E D S D V E M V E D D S R K E M T A A C T P R R R I I N L T S V L S L Q E E I N E Q G H E V L R E M L H N H S F V G C V N P Q W A L A Q H Q T K L Y L L N T T K L S E E L F Y Q I L I Y D F A N F G V L R L S E P A P L F D L A M L A L D S P E S G W T E E D G P K E G L A E Y I V E F L K K K A E M L A D Y F S L E I D E E G N L I G L P L L I D N Y V P P L E G L P I F I L R L A T E V N W D E E K E C F E S L S K E C A M F Y S I R K Q Y I S E E S T L S G Q Q S E V P G S I P N S W K W T V E H I V Y K A L R S H I L P P K H F T E D G N I L Q L A N L P D L Y K V F E R C
- Accession
- P40692
- Protein length
- Full Length
- Predicted molecular weight
- 108.9 kDa
- Amino acids
- 1 to 756
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
- Sequence similarities
Belongs to the DNA mismatch repair MutL/HexB family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MLH1 also known as MutL homolog 1 is a protein involved in DNA mismatch repair an important mechanism for maintaining genetic stability. It has a molecular weight of approximately 87 kDa. This protein is expressed in various tissues but is most abundant in the colonic epithelium and endometrium. MLH1 acts mechanically by forming heterodimers with other proteins collaborating in correcting errors that occur during DNA replication.
- Biological function summary
The function of MLH1 involves its role in the mismatch repair (MMR) system. It is part of a complex with PMS2 forming a heterodimer known as MutLα which is essential for the repair process. This complex scans newly synthesized DNA for mispaired bases and initiates repair preserving genomic integrity. The proper function of MLH1 and its interaction with PMS2 ensures that DNA replication errors do not accumulate and cause harmful mutations.
- Pathways
MLH1 operates within the mismatch repair pathway and interacts closely with MLH3 and PMS2 proteins. It plays a critical role in the recognition and repair of mismatched bases that occur during DNA replication particularly in the G2 phase of the cell cycle. Through its involvement in the mismatch repair pathway MLH1 is connected to cell cycle regulation and the DNA damage response pathway.
- Associated diseases and disorders
MLH1 mutations are closely linked to Lynch syndrome and sporadic colorectal cancer. Lynch syndrome a hereditary condition significantly raises the risk of colorectal cancer and other cancers due to defective DNA mismatch repair. MLH1 mutations often lead to the loss of MLH1 protein expression particularly observed in MLH1 IHC staining. Additionally in colorectal cancer the MLH1 protein may interact with APC and TP53 playing a role in cancer progression and tumorigenesis.
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1 product image
SDS-PAGE - Recombinant Human MLH1 protein (ab131924)
12.5% SDS-PAGE analysis of ab131924 stained with Coomassie Blue.
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