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Recombinant Human MLH1 protein is a Human Full Length protein, in the 1 to 756 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.

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Images

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

SDS-PAGE, ELISA, WB

Biologically active

No

Amino acid sequence

M S F V A G V I R R L D E T V V N R I A A G E V I Q R P A N A I K E M I E N C L D A K S T S I Q V I V K E G G L K L I Q I Q D N G T G I R K E D L D I V C E R F T T S K L Q S F E D L A S I S T Y G F R G E A L A S I S H V A H V T I T T K T A D G K C A Y R A S Y S D G K L K A P P K P C A G N Q G T Q I T V E D L F Y N I A T R R K A L K N P S E E Y G K I L E V V G R Y S V H N A G I S F S V K K Q G E T V A D V R T L P N A S T V D N I R S I F G N A V S R E L I E I G C E D K T L A F K M N G Y I S N A N Y S V K K C I F L L F I N H R L V E S T S L R K A I E T V Y A A Y L P K N T H P F L Y L S L E I S P Q N V D V N V H P T K H E V H F L H E E S I L E R V Q Q H I E S K L L G S N S S R M Y F T Q T L L P G L A G P S G E M V K S T T S L T S S S T S G S S D K V Y A H Q M V R T D S R E Q K L D A F L Q P L S K P L S S Q P Q A I V T E D K T D I S S G R A R Q Q D E E M L E L P A P A E V A A K N Q S L E G D T T K G T S E M S E K R G P T S S N P R K R H R E D S D V E M V E D D S R K E M T A A C T P R R R I I N L T S V L S L Q E E I N E Q G H E V L R E M L H N H S F V G C V N P Q W A L A Q H Q T K L Y L L N T T K L S E E L F Y Q I L I Y D F A N F G V L R L S E P A P L F D L A M L A L D S P E S G W T E E D G P K E G L A E Y I V E F L K K K A E M L A D Y F S L E I D E E G N L I G L P L L I D N Y V P P L E G L P I F I L R L A T E V N W D E E K E C F E S L S K E C A M F Y S I R K Q Y I S E E S T L S G Q Q S E V P G S I P N S W K W T V E H I V Y K A L R S H I L P P K H F T E D G N I L Q L A N L P D L Y K V F E R C

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Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

ELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.

Alternative names

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Recombinant Human MLH1 protein is a Human Full Length protein, in the 1 to 756 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.

Alternative names

Key facts

Expression system

Wheat germ

Applications

SDS-PAGE, ELISA, WB

Accession
P40692-1
Animal free

No

Species

Human

Concentration
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Storage buffer

pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione

Sequence info

Amino acid sequence

M S F V A G V I R R L D E T V V N R I A A G E V I Q R P A N A I K E M I E N C L D A K S T S I Q V I V K E G G L K L I Q I Q D N G T G I R K E D L D I V C E R F T T S K L Q S F E D L A S I S T Y G F R G E A L A S I S H V A H V T I T T K T A D G K C A Y R A S Y S D G K L K A P P K P C A G N Q G T Q I T V E D L F Y N I A T R R K A L K N P S E E Y G K I L E V V G R Y S V H N A G I S F S V K K Q G E T V A D V R T L P N A S T V D N I R S I F G N A V S R E L I E I G C E D K T L A F K M N G Y I S N A N Y S V K K C I F L L F I N H R L V E S T S L R K A I E T V Y A A Y L P K N T H P F L Y L S L E I S P Q N V D V N V H P T K H E V H F L H E E S I L E R V Q Q H I E S K L L G S N S S R M Y F T Q T L L P G L A G P S G E M V K S T T S L T S S S T S G S S D K V Y A H Q M V R T D S R E Q K L D A F L Q P L S K P L S S Q P Q A I V T E D K T D I S S G R A R Q Q D E E M L E L P A P A E V A A K N Q S L E G D T T K G T S E M S E K R G P T S S N P R K R H R E D S D V E M V E D D S R K E M T A A C T P R R R I I N L T S V L S L Q E E I N E Q G H E V L R E M L H N H S F V G C V N P Q W A L A Q H Q T K L Y L L N T T K L S E E L F Y Q I L I Y D F A N F G V L R L S E P A P L F D L A M L A L D S P E S G W T E E D G P K E G L A E Y I V E F L K K K A E M L A D Y F S L E I D E E G N L I G L P L L I D N Y V P P L E G L P I F I L R L A T E V N W D E E K E C F E S L S K E C A M F Y S I R K Q Y I S E E S T L S G Q Q S E V P G S I P N S W K W T V E H I V Y K A L R S H I L P P K H F T E D G N I L Q L A N L P D L Y K V F E R C

Accession

P40692

Protein length

Full Length

Predicted molecular weight

108.9 kDa

Amino acids

1 to 756

Nature

Recombinant

Tags

GST tag N-Terminus

Specifications

Form

Liquid

General info

Function

Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.

Sequence similarities

Belongs to the DNA mismatch repair MutL/HexB family.

Subcellular localisation

Nucleus

Storage

Shipped at conditions

Dry Ice

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Supplementary info

Biological function summary

The function of MLH1 involves its role in the mismatch repair (MMR) system. It is part of a complex with PMS2 forming a heterodimer known as MutLα which is essential for the repair process. This complex scans newly synthesized DNA for mispaired bases and initiates repair preserving genomic integrity. The proper function of MLH1 and its interaction with PMS2 ensures that DNA replication errors do not accumulate and cause harmful mutations.

Activity summary

MLH1 also known as MutL homolog 1 is a protein involved in DNA mismatch repair an important mechanism for maintaining genetic stability. It has a molecular weight of approximately 87 kDa. This protein is expressed in various tissues but is most abundant in the colonic epithelium and endometrium. MLH1 acts mechanically by forming heterodimers with other proteins collaborating in correcting errors that occur during DNA replication.

Pathways

MLH1 operates within the mismatch repair pathway and interacts closely with MLH3 and PMS2 proteins. It plays a critical role in the recognition and repair of mismatched bases that occur during DNA replication particularly in the G2 phase of the cell cycle. Through its involvement in the mismatch repair pathway MLH1 is connected to cell cycle regulation and the DNA damage response pathway.

Associated diseases and disorders

MLH1 mutations are closely linked to Lynch syndrome and sporadic colorectal cancer. Lynch syndrome a hereditary condition significantly raises the risk of colorectal cancer and other cancers due to defective DNA mismatch repair. MLH1 mutations often lead to the loss of MLH1 protein expression particularly observed in MLH1 IHC staining. Additionally in colorectal cancer the MLH1 protein may interact with APC and TP53 playing a role in cancer progression and tumorigenesis.

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1 product image

  • SDS-PAGE - Recombinant Human MLH1 protein (ab131924), expandable thumbnail

    SDS-PAGE - Recombinant Human MLH1 protein (ab131924)

    12.5% SDS-PAGE analysis of ab131924 stained with Coomassie Blue.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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