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AB135021

Recombinant human MLL + ASH2L + RBBP5 + WDR5 protein (6x His tag N-Terminus)

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Recombinant human MLL + ASH2L + RBBP5 + WDR5 protein (6x His tag N-Terminus) is a Human Fragment protein, in the 3745 to 3969 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, FuncS.

View Alternative Names

BIG3, WDR5, WD repeat-containing protein 5, BMP2-induced 3-kb gene protein, ASH2L1, ASH2L, Set1/Ash2 histone methyltransferase complex subunit ASH2, ASH2-like protein, ALL1, CXXC7, HRX, HTRX, MLL, MLL1, TRX1, KMT2A, Histone-lysine N-methyltransferase 2A, Lysine N-methyltransferase 2A, ALL-1, CXXC-type zinc finger protein 7, Cysteine methyltransferase KMT2A, Myeloid/lymphoid or mixed-lineage leukemia, Myeloid/lymphoid or mixed-lineage leukemia protein 1, Trithorax-like protein, Zinc finger protein HRX, RBQ3, RBBP5, Retinoblastoma-binding protein 5, RBBP-5, Retinoblastoma-binding protein RBQ-3

2 Images
Functional Studies - Recombinant human MLL + ASH2L + RBBP5 + WDR5 protein (6x His tag N-Terminus) (AB135021)
  • FuncS

Unknown

Functional Studies - Recombinant human MLL + ASH2L + RBBP5 + WDR5 protein (6x His tag N-Terminus) (AB135021)

Graph demonstrating the activity of ab135021

SDS-PAGE - Recombinant human MLL + ASH2L + RBBP5 + WDR5 protein (6x His tag N-Terminus) (AB135021)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human MLL + ASH2L + RBBP5 + WDR5 protein (6x His tag N-Terminus) (AB135021)

4-20% SDS-PAGE analysis of ab135021 stained with Coomassie Blue.

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Active

Accession

P61964

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Preservative: 1.7% Imidazole Constituents: 10% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 0.29% Tris HCl, 0.03% TCEP HCl, 0.00001% Zinc chloride

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Complementary Products

Primary Antibodies

AB32400

Anti-KMT2A / MLL antibody [mmN4.4]

BOND RX™ Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT2A / MLL antibody [mmN4.4] (AB32400)

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Proteins & Peptides

AB198139

Recombinant human TAF1 protein (GST tag N-Terminus)

Functional Studies - Recombinant human TAF1 protein (GST tag N-Terminus) (AB198139)

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Proteins & Peptides

AB95933

Recombinant human XIAP protein (DDDDK tag N-Terminus)

Functional Studies - Recombinant human XIAP protein (DDDDK tag N-Terminus) (AB95933)

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Primary Antibodies

AB154755

Anti-RbBP5 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RbBP5 antibody (AB154755)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Useful for the study of enzymekinetics, screening inhibitors, and selectivityprofiling.</p>" } } }
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Product details

50 μl reaction mix (50 mM Tris, pH 8.8, 5 mM MgCl2, 4 M DTT, 20 μM Sadenosylmethionine, and 5-50 ng MLL complex) add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated K4 residue of histone H3, incubate 1 hr. Then, incubate 30 minutes with the secondary HRP labeled antibody. Finally, add HRP chemiluminescent substrate and read luminescence.
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Sequence info

[{"sequence":"MHHHHHHFRFHKPEEANEPPLNPHGSARAEVHLRKSAFDMFNFLASKHRQPPEYNPNDEEEEEVQLKSARRATSMDLPMPMRFRHLKKTSKEAVGVYRSPIHGRGLFCKRNIDAGEMVIEYAGNVIRSIQTDKREKYYDSKGIGCYMFRIDDSEVVDATMHGNAARFINHSCEPNCYSRVINIDGQKHIVIFAMRKIYRGEELTYDYKFPIEDASNKLPCNCGAKKCRKFLN","proteinLength":"Fragment","predictedMolecularWeight":"27 kDa","actualMolecularWeight":null,"aminoAcidEnd":3969,"aminoAcidStart":3745,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q03164","tags":[{"tag":"6x His","terminus":"N-Terminus"}]},{"sequence":"MHHHHHHATEEKKPETEAARAQPTPSSSATQSKPTPVKPNYALKFTLAGHTKAVSSVKFSPNGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNLVYIWNLQTKEIVQKLQGHTDVVISTACHPTENIIASAALENDKTIKLWKSDC","proteinLength":"Full Length","predictedMolecularWeight":"37.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P61964","tags":[{"tag":"6x His","terminus":"N-Terminus"}]},{"sequence":"MHHHHHHDTQAGSVDEENGRQLGEVELQCGICTKWFTADTFGIDTSSCLPFMTNYSFHCNVCHHSGNTYFLRKQANLKEMCLSALANLTWQSRTQDEHPKTMFSKDKDIIPFIDKYWECMTTRQRPGKMTWPNNIVKTMSKERDVFLVKEHPDPGSKDPEEDYPKFGLLDQDLSNIGPAYDNQKQSSAVSTSGNLNGGIAAGSSGKGRGAKRKQQDGGTTGTTKKARSDPLFSAQRLPPHGYPLEHPFNKDGYRYILAEPDPHAPDPEKLELDCWAGKPIPGDLYRACLYERVLLALHDRAPQLKISDDRLTVVGEKGYSMVRASHGVRKGAWYFEITVDEMPPDTAARLGWSQPLGNLQAPLGYDKFSYSWRSKKGTKFHQSIGKHYSSGYGQGDVLGFYINLPEDTETAKSLPDTYKDKALIKFKSYLYFEEKDFVDKAEKSLKQTPHSEIIFYKNGVNQGVAYKDIFEGVYFPAISLYKSCTVSINFGPCFKYPPKDLTYRPMSDMGWGAVVEHTLADVLYHVETEVDGRRSPPWEP","proteinLength":"Fragment","predictedMolecularWeight":"61 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9UBL3","tags":[{"tag":"6x His","terminus":"N-Terminus"}]},{"sequence":"MHHHHHHNLELLESFGQNYPEEADGTLDCISMALTCTFNRWGTLLAVGCNDGRIVIWDFLTRGIAKIISAHIHPVCSLCWSRDGHKLVSASTDNIVSQWDVLSGDCDQRFRFPSPILKVQYHPRDQNKVLVCPMKSAPVMLTLSDSKHVVLPVDDDSDLNVVASFDRRGEYIYTGNAKGKILVLKTDSQDLVASFRVTTGTSNTTAIKSIEFARKGSCFLINTADRIIRVYDGREILTCGRDGEPEPMQKLQDLVNRTPWKKCCFSGDGEYIVAGSARQHALYIWEKSIGNLVKILHGTRGELLLDVAWHPVRPIIASISSGVVSIWAQNQVENWSAFAPDFKELDENVEYEERESEFDIEDEDKSEPEQTGADAAEDEEVDVTSVDPIAAFCSSDEELEDSKALLYLPIAPEVEDPEENPYGPPPDAVQTSLMDEGASSEKKRQSSADGSQPPKKKPKTTNIELQGVPNDEVHPLLGVKGDGKSKKKQAGRPKGSKGKEKDSPFKPKLYKGDRGLPLEGSAKGKVQAELSQPLTAGGAISELL","proteinLength":"Full Length","predictedMolecularWeight":"60 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q15291","tags":[{"tag":"6x His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MLL + ASH2L + RBBP5 + WDR5 complex also known as the SET1/COMPASS complex mechanically acts in gene regulation through histone modification. This multiprotein complex includes MLL (Myeloid-Lymphoid Leukemia) ASH2L (Absent Small or Homeotic 2-Like) RBBP5 (Retinoblastoma-Binding Protein 5) and WDR5 (WD Repeat Domain 5) with a combined mass that varies around 600 kDa. It expresses widely in different human tissues with notable activity in cells undergoing differentiation due to its essential role in chromatin remodeling and transcriptional activation.
Biological function summary

This complex plays an important role in the process of epigenetic regulation within cells. It functions by specifically catalyzing the methylation of histone H3 on lysine 4 (H3K4) which is associated with transcriptional activation. As a core component of the histone modification machinery the MLL complex significantly influences gene expression patterns. This activity regulates critical developmental and cellular differentiation processes highlighting its importance in maintaining healthy cell function.

Pathways

The MLL + ASH2L + RBBP5 + WDR5 complex integrates within the epigenetic signaling and transcriptional regulatory pathways. Its activity intersects with the Wnt signaling pathway which is essential for regulating embryonic development and cell proliferation. Additionally it interfaces with the Notch signaling pathway closely linked to cell differentiation processes. Proteins such as MEN1 (Menin 1) and others within the epigenetic network interact with this complex to exert their effects on downstream gene targets.

The aberrant function of the MLL + ASH2L + RBBP5 + WDR5 complex associates with mixed-lineage leukemia due to mutations or translocations involving MLL. This complex also relates to other malignancies as dysregulation of histone modification can contribute to oncogenesis. In particular mismanagement of the complex's activity can lead to poor outcomes in acute leukemia. It's connection to the protein DOT1L (Disruptor of Telomeric Silencing 1-Like) involved in another methyltransferase activity further emphasizes its relevance to diseases related to improper gene regulation.
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Specifications

Form

Liquid

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General info

Function

Contributes to histone modification (PubMed : 16600877, PubMed : 16829960, PubMed : 19103755, PubMed : 19131338, PubMed : 19556245, PubMed : 20018852). May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4' (PubMed : 16829960). As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3 (PubMed : 19556245). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed : 18840606). As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed : 19103755, PubMed : 20018852). May regulate osteoblasts differentiation (By similarity). In association with RBBP5 and ASH2L, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (PubMed : 21220120, PubMed : 22266653).

Sequence similarities

Belongs to the WD repeat WDR5/wds family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Contributes to histone modification (PubMed : 16600877, PubMed : 16829960, PubMed : 19103755, PubMed : 19131338, PubMed : 19556245, PubMed : 20018852). May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4' (PubMed : 16829960). As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3 (PubMed : 19556245). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed : 18840606). As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed : 19103755, PubMed : 20018852). May regulate osteoblasts differentiation (By similarity). In association with RBBP5 and ASH2L, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (PubMed : 21220120, PubMed : 22266653).
See full target information WDR5

Additional targets

ASH2L,RBBP5,KMT2A
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