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AB99217

Recombinant Human MMAB protein

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Recombinant Human MMAB protein is a Human Full Length protein, in the 33 to 250 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Corrinoid adenosyltransferase MMAB, ATP:co(I)rrinoid adenosyltransferase MMAB, Methylmalonic aciduria type B protein, MMAB

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SDS-PAGE - Recombinant Human MMAB protein (AB99217)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MMAB protein (AB99217)

15% SDS-PAGE analysis of 3μg ab99217.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q96EY8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMQSRGPQGVEDGDRPQPSSKTPRIPKIYTKTGDKGFSSTFTGERRPKDDQVFEAVGTTDELSSAIGFALELVTEKGHTFAEELQKIQCTLQDVGSALATPCSSAREAHLKYTTFKAGPILELEQWIDKYTSQLPPLTAFILPSGGKISSALHFCRAVCRRAERRVVPLVQMGETDANVAKFLNRLSDYLFTLARYAAMKEGNQEKIYKKNDPSAESEGL","proteinLength":"Full Length","predictedMolecularWeight":"26.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":250,"aminoAcidStart":33,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96EY8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MMAB also known as methylmalonic aciduria type B protein plays a critical role in the conversion of certain molecules within the mitochondria. This protein weighing approximately 38 kDa assists in the conversion of vitamin B12 into adenosylcobalamin an active coenzyme form. MMAB is present in many tissues with significant expression in liver and kidney where significant mitochondrial activities occur. Methylmalonyl-CoA a byproduct needing processing for energy production relies on MMAB for transformation.
Biological function summary

MMAB is indispensable for vitamin B12 metabolism and mitochondrial function. This protein acts within a larger enzymatic complex that converts methylmalonyl-CoA to succinyl-CoA. By playing a role in this conversion MMAB facilitates the Krebs cycle an essential biochemical pathway for energy production. Its interaction with other proteins like methylmalonyl-CoA mutase ensures proper biochemical transformations highlighting its importance in metabolic processes.

Pathways

Methylmalonic acidemia results from disruptions in the MMAB-mediated pathway notably affecting energy metabolism. This protein integrates into the mitochondrial pathway that handles odd-chain fatty acids and amino acid breakdown. The effective collaboration of MMAB with methylmalonyl-CoA mutase ensures efficient functioning within these pathways linking MMAB to essential metabolic activities like gluconeogenesis and energy production.

Mutations in MMAB contribute to methylmalonic acidemia a metabolic disorder characterized by accumulation of methylmalonic acid in the body. This condition can lead to developmental delay metabolic crisis and other severe symptoms if untreated. Alongside methylmalonyl-CoA mutase MMAB mutations impact vitamin B12-related pathways highlighting their joint role in this genetic disorder. Early diagnosis and treatment targeting this pathway help mitigate symptoms related to these enzymatic dysfunctions.
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Specifications

Form

Liquid

Additional notes

ab99217 was purified using conventional chromatography techniques.

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General info

Function

Converts cob(I)alamin to adenosylcobalamin (adenosylcob(III)alamin), a coenzyme for methylmalonyl-CoA mutase, therefore participates in the final step of the vitamin B12 conversion (PubMed : 12514191). Generates adenosylcobalamin (AdoCbl) and directly delivers the cofactor to MUT in a transfer that is stimulated by ATP-binding to MMAB and gated by MMAA (Probable).

Sequence similarities

Belongs to the Cob(I)alamin adenosyltransferase family.

Subcellular localisation

Mitochondrion

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Product protocols

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Target data

Converts cob(I)alamin to adenosylcobalamin (adenosylcob(III)alamin), a coenzyme for methylmalonyl-CoA mutase, therefore participates in the final step of the vitamin B12 conversion (PubMed : 12514191). Generates adenosylcobalamin (AdoCbl) and directly delivers the cofactor to MUT in a transfer that is stimulated by ATP-binding to MMAB and gated by MMAA (Probable).
See full target information MMAB
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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
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Primary Antibodies

AB174831

Anti-MMAB antibody [EPR8695]

RabMAb|Recombinant

Western blot - Anti-MMAB antibody [EPR8695] (AB174831)

  • 0
  • 0
Secondary Antibodies

AB150121

Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (AB150121)

  • 5
  • 5
Secondary Antibodies

AB99695

Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain

  • 0
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