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AB198437

Recombinant human MMP1 protein (Active)

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Recombinant human MMP1 protein (Active) is a Human Full Length protein, in the 20 to 469 aa range, expressed in HEK 293 cells, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

CLG, MMP1, Interstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1

2 Images
Functional Studies - Recombinant human MMP1 protein (Active) (AB198437)
  • FuncS

Supplier Data

Functional Studies - Recombinant human MMP1 protein (Active) (AB198437)

Activity assay of ab198437.

SDS-PAGE - Recombinant human MMP1 protein (Active) (AB198437)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human MMP1 protein (Active) (AB198437)

4-20% SDS-PAGE analysis of 2.4 μg ab198437 with Coomassie staining.

Key facts

Purity

>90% SDS-PAGE

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

≥ 105 pmole/min/μg.

Activation: This is a proform enzyme and requires activation prior to testing activity. Dilute the enzyme to 100 ng/ml in a solution containing 50 mM HEPES, pH 7.4, 10 mM CaCl2, 0.05% Brij-35, and 1 mM APMA (amino-phenyl mercuric acetate). Incubate at 37°C for 2 hours.
Assay conditions: Reaction mixture with 10 μM 390 MMP substrate 1. Incubate for 30 minutes at room temperature. Fluorescence intensity is measured at exc328/em393.

Accession

P03956

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Preservative: 1.36% Imidazole Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Tris HCl, 0.64% Sodium chloride, 0.017% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"FPATLETQEQDVDLVQKYLEKYYNLKNDGRQVEKRRNSGPVVEKLKQMQEFFGLKVTGKPDAETLKVMKQPRCGVPDVAQFVLTEGNPRWEQTHLTYRIENYTPDLPRADVDHAIEKAFQLWSNVTPLTFTKVSEGQADIMISFVRGDHRDNSPFDGPGGNLAHAFQPGPGIGGDAHFDEDERWTNNFREYNLHRVAAHELGHSLGLSHSTDIGALMYPSYTFSGDVQLAQDDIDGIQAIYGRSQNPVQPIGPQTPKACDSKLTFDAITTIRGEVMFFKDRFYMRTNPFYPEVELNFISVFWPQLPNGLEAAYEFADRDEVRFFKGNKYWAVQGQNVLHGYPKDIYSSFGFPRTVKHIDAALSEENTGKTYFFVANKYWRYDEYKRSMDPGYPKMIAHDFPGIGHKVDAVFMKDGFFYFFHGTRQYKFDPKTKRILTLQKANSWFNCRKNHHHHHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"53 kDa","actualMolecularWeight":null,"aminoAcidEnd":469,"aminoAcidStart":20,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P03956","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MMP1 also known as collagenase-1 is an enzyme belonging to the matrix metalloproteinase (MMP) family. It is responsible for the degradation of type I II and III collagens making it an important player in collagen turnover. MMP1 has a molecular weight of approximately 54 kDa. This enzyme is expressed in various tissues including skin lung and reproductive organs primarily during tissue remodeling and repair. Scientists often use MMP1 ELISA kits and MMP1 inhibitors to study its expression and activity levels.
Biological function summary

MMP1 plays a significant role in the modulation of the extracellular matrix. It facilitates cellular processes by breaking down structural proteins and allowing for cell migration proliferation and differentiation. Although it acts independently MMP1 works in harmony with other MMP family members to maintain extracellular matrix homeostasis and is often linked to tissue remodeling complexes. This makes the MMP1 a valuable target for researchers interested in tissue repair and fibrosis.

Pathways

Studies show MMP1 involvement in critical processes such as the inflammatory response and wound healing. In the inflammatory pathway MMP1 expression is regulated by cytokines that respond to tissue injury. Within the wound healing pathway this enzyme interacts with proteins like transforming growth factor-beta (TGF-beta) to coordinate the repair process by remodeling extracellular matrix components. This illustrates the interconnected nature of MMP1 with significant regulatory proteins.

MMP1 has significant associations with osteoarthritis and cancer metastasis. MMP1 contributes to the breakdown of cartilage in osteoarthritis highlighting its role in disease progression and joint degradation. In the context of cancer elevated MMP1 expression correlates with the metastatic potential where it assists tumor invasion by degrading the surrounding matrix. MMP1's involvement with other MMPs including MMP2 and MMP9 highlights its importance in these pathological conditions making it a therapeutic target in related research.
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Specifications

Form

Liquid

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General info

Function

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (PubMed : 1645757, PubMed : 2153297, PubMed : 2557822). In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity (PubMed : 16807369).

Sequence similarities

Belongs to the peptidase M10A family.

Post-translational modifications

Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.. Tyrosine phosphorylated in platelets by PKDCC/VLK.

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Product protocols

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Target data

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (PubMed : 1645757, PubMed : 2153297, PubMed : 2557822). In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity (PubMed : 16807369).
See full target information MMP1
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