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AB131994

Recombinant Human MMP12 protein (GST tag N-Terminus)

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(1 Publication)

Recombinant Human MMP12 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 470 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

HME, MMP12, Macrophage metalloelastase, MME, Macrophage elastase, Matrix metalloproteinase-12, ME, hME, MMP-12

1 Images
SDS-PAGE - Recombinant Human MMP12 protein (GST tag N-Terminus) (AB131994)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MMP12 protein (GST tag N-Terminus) (AB131994)

12.5% SDS-PAGE using ab131994 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, SDS-PAGE, ELISA

applications

Biologically active

No

Accession

P39900

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB52897

Anti-MMP12 antibody [EP1261Y]

RabMAb|Recombinant

Immunoprecipitation - Anti-MMP12 antibody [EP1261Y] (AB52897)

  • 0
  • 0
Assay Kits

AB246540

Mouse MMP12 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse MMP12 ELISA Kit (AB246540)

  • 0
  • 0
Proteins & Peptides

AB237007

Recombinant Human p53 protein

Mass Spectrometry - Recombinant Human p53 protein (AB237007)

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Proteins & Peptides

AB316609

Recombinant Human Neurogranin Protein Standard (His tag)

Sandwich ELISA - Recombinant Human Neurogranin Protein Standard (His tag) (AB316609)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MKFLLILLLQATASGALPLNSSTSLEKNNVLFGERYLEKFYGLEINKLPVTKMKYSGNLMKEKIQEMQHFLGLKVTGQLDTSTLEMMHAPRCGVPDVHHFREMPGGPVWRKHYITYRINNYTPDMNREDVDYAIRKAFQVWSNVTPLKFSKINTGMADILVVFARGAHGDFHAFDGKGGILAHAFGPGSGIGGDAHFDEDEFWTTHSGGTNLFLTAVHEIGHSLGLGHSSDPKAVMFPTYKYVDINTFRLSADDIRGIQSLYGDPKENQRLPNPDNSEPALCDPNLSFDAVTTVGNKIFFFKDRFFWLKVSERPKTSVNLISSLWPTLPSGIEAAYEIEARNQVFLFKDDKYWLISNLRPEPNYPKSIHSFGFPNFVKKIDAAVFNPRFYRTYFFVDNQYWRYDERRQMMDPGYPKLITKNFQGIGPKIDAVFYSKNKYYYFFQGSNQFEYDFLLQRITKTLKSNSWFGC","proteinLength":"Full Length","predictedMolecularWeight":"80.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":470,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P39900","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Matrix metallopeptidase 12 commonly known as MMP12 or macrophage elastase is an enzyme with a molecular mass of approximately 54 kDa. Found mainly in macrophages MMP12 belongs to the matrix metallopeptidase family. It functions to break down extracellular matrix proteins specifically elastin. Besides macrophages it sees expression in other tissues including the lungs and skin but it holds particular importance in immune cell-related environments.
Biological function summary

MMP12 plays an important role in tissue remodeling and repair processes. It interacts with other proteases within the extracellular matrix. The degradation of elastin by MMP12 contributes to structural organization and it participates in immune response modulation. As part of the matrix metalloproteinase family MMP12 takes part in the larger proteolytic system which comprises multiple enzymes working in concert.

Pathways

MMP12 is an essential factor in the extracellular matrix degradation pathway. This pathway regulates tissue remodeling and inflammatory response. MMP12 independently breaks down elastin while it interacts with proteins like MMP2 and MMP9 which assist in remodeling processes. It also contributes to the regulation of cytokines that orchestrate immune functions.

Overexpression of MMP12 is strongly linked to chronic obstructive pulmonary disease (COPD) and atherosclerosis. In COPD MMP12's breakdown of elastin contributes to tissue damage in the lung. In atherosclerosis the enzyme accelerates plaque destabilization impacting cardiovascular health. Additionally MMP12 shares functional relationships with proteins such as TIMPs (tissue inhibitors of metalloproteinases) which regulate its activity and play roles in disease pathogenesis.
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Specifications

Form

Liquid

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General info

Function

May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.

Sequence similarities

Belongs to the peptidase M10A family.

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Product protocols

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Target data

May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
See full target information MMP12
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biochimica et biophysica acta 1842:1379-84 PubMed24815357

2014

Correlation between MMP-9 and extracellular cytokine HMGB1 in prediction of human ischemic stroke outcome.

Applications

Unspecified application

Species

Unspecified reactive species

Nelly Sapojnikova,Tamar Kartvelishvili,Nino Asatiani,Vitaly Zinkevich,Iagor Kalandadze,Darejan Gugutsidze,Roman Shakarishvili,Alexander Tsiskaridze
View all publications
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Product promise

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