Recombinant Human MMP13 protein is a Human Fragment protein, in the 104 to 471 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S Y N V F P R T L K W S K M N L T Y R I V N Y T P D M T H S E V E K A F K K A F K V W S D V T P L N F T R L H D G I A D I M I S F G I K E H G D F Y P F D G P S G L L A H A F P P G P N Y G G D A H F D D D E T W T S S S K G Y N L F L V A A H E F G H S L G L D H S K D P G A L M F P I Y T Y T G K S H F M L P D D D V Q G I Q S L Y G P G D E D P N P K H P K T P D K C D P S L S L D A I T S L R G E T M I F K D R F F W R L H P Q Q V D A E L F L T K S F W P E L P N R I D A A Y E H P S H D L I F I F R G R K F W A L N G Y D I L E G Y P K K I S E L G L P K E V K K I S A A V H F E D T G K T L L F S G N Q V W R Y D D T N H I M D K D Y P R L I E E D F P G I G D K V D A V Y E K N G Y I Y F F N G P I Q F E Y S I W S N R I V R V M P A N S I L W C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.
Alternative names
Collagenase 3, Matrix metalloproteinase-13, MMP-13, MMP13
Recommended products
Recombinant Human MMP13 protein is a Human Fragment protein, in the 104 to 471 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P45452-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris-HCl buffer, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S Y N V F P R T L K W S K M N L T Y R I V N Y T P D M T H S E V E K A F K K A F K V W S D V T P L N F T R L H D G I A D I M I S F G I K E H G D F Y P F D G P S G L L A H A F P P G P N Y G G D A H F D D D E T W T S S S K G Y N L F L V A A H E F G H S L G L D H S K D P G A L M F P I Y T Y T G K S H F M L P D D D V Q G I Q S L Y G P G D E D P N P K H P K T P D K C D P S L S L D A I T S L R G E T M I F K D R F F W R L H P Q Q V D A E L F L T K S F W P E L P N R I D A A Y E H P S H D L I F I F R G R K F W A L N G Y D I L E G Y P K K I S E L G L P K E V K K I S A A V H F E D T G K T L L F S G N Q V W R Y D D T N H I M D K D Y P R L I E E D F P G I G D K V D A V Y E K N G Y I Y F F N G P I Q F E Y S I W S N R I V R V M P A N S I L W C
- Accession
- P45452
- Protein length
- Fragment
- Predicted molecular weight
- 44.7 kDa
- Amino acids
- 104 to 471
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab177626 was purified by using conventional chromatography techniques.
General info
- Function
Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.
- Sequence similarities
Belongs to the peptidase M10A family.
- Post-translational modifications
The proenzyme is activated by removal of the propeptide; this cleavage can be effected by other matrix metalloproteinases, such as MMP2, MMP3 and MMP14 and may involve several cleavage steps. Cleavage can also be autocatalytic, after partial maturation by another protease or after treatment with 4-aminophenylmercuric acetate (APMA) (in vitro).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Matrix metallopeptidase 13 (MMP-13) also called collagenase 3 is an enzyme with an approximate molecular mass of 54 kDa. It plays a significant role in the breakdown of extracellular matrix components particularly collagen. MMP-13 is expressed in several tissues including cartilage skin and bone. Its expression sees an increase during tissue remodeling and in pathological conditions. MMP-13 also participates in processes like wound healing and embryonic development making it a focal point for understanding tissue dynamics.
- Biological function summary
Matrix metallopeptidase 13 contributes extensively to the degradation of collagen type II a major component of cartilage. As a zinc-dependent endopeptidase MMP-13 is part of the MMP family which facilitates the remodeling of the extracellular matrix. MMP-13 is involved in the proteolytic cascade working in conjunction with other MMPs and elastase to mediate tissue repair and turnover. It does not form complexes but acts in concert with other enzymes to execute its physiological functions effectively.
- Pathways
Matrix metallopeptidase 13 significance is most noted in the cartilage degradation pathway. It interacts with other MMPs such as MMP-1 and MMP-9 to efficiently break down extracellular matrix components. These interactions highlight its role in the regulation of matrix metalloproteinase activity within the connective tissue degradation pathway. These pathways are key during normal connective tissue remodeling and in pathological processes illustrating the essential roles of MMPs in maintaining tissue homeostasis.
- Associated diseases and disorders
Matrix metallopeptidase 13 has strong associations with osteoarthritis and rheumatoid arthritis. It contributes to cartilage destruction intensifying the progression of these disorders due to its potent collagenolytic activity. Other proteins like MMP-9 and MMP-14 are also involved in these processes potentially amplifying tissue damage in affected joints. Understanding how MMP-13 and related proteins drive these diseases offers potential therapeutic targets for slowing disease progression and enhancing joint health.
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SDS-PAGE - Recombinant Human MMP13 protein (ab177626)
15% SDS-PAGE analysis of ab177626 (3 μg).
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