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Recombinant human MMP2 protein (Active) is a Human Full Length protein, in the 110 to 660 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for FuncS.

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Purity

>=95% SDS-PAGE

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

FuncS

Biologically active

Yes

Sequence

Y N F F P R K P K W D K N Q I T Y R I I G Y T P D L D P E T V D D A F A R A F Q V W S D V T P L R F S R I H D G E A D I M I N F G R W E H G D G Y P F D G K D G L L A H A F A P G T G V G G D S H F D D D E L W T L G E G Q V V R V K Y G N A D G E Y C K F P F L F N G K E Y N S C T D T G R S D G F L W C S T T Y N F E K D G K Y G F C P H E A L F T M G G N A E G Q P C K F P F R F Q G T S Y D S C T T E G R T D G Y R W C G T T E D Y D R D K K Y G F C P E T A M S T V G G N S E G A P C V F P F T F L G N K Y E S C T S A G R S D G K M W C A T T A N Y D D D R K W G F C P D Q G Y S L F L V A A H E F G H A M G L E H S Q D P G A L M A P I Y T Y T K N F R L S Q D D I K G I Q E L Y G A S P D I D L G T G P T P T L G P V T P E I C K Q D I V F D G I A Q I R G E I F F F K D R F I W R T V T P R D K P M G P L L V A T F W P E L P E K I D A V Y E A P Q E E K A V F F A G N E Y W I Y S A S T L E R G Y P K P L T S L G L P P D V Q R V D A A F N W S K N K K T Y I F A G D K F W R Y N E V K K K M D P G F P K L I A D A W N A I P D N L D A V V D L Q G G G H S Y F F K G A Y Y L K L E N Q S L K S V K F G S I K S D W L G C

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Shipping To:
United States

Reactivity data

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

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Recombinant human MMP2 protein (Active) is a Human Full Length protein, in the 110 to 660 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for FuncS.

Key facts

Purity

>=95% SDS-PAGE

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Applications

FuncS

Biological activity

Fully active determined by its ability to cleave human MMP2 fluorogenic peptide substrate (MCa-PLGL-Dpa-AR-NH2.

Predicted molecular weight

63 kDa

Amino acids

110 to 660

Sequence

Y N F F P R K P K W D K N Q I T Y R I I G Y T P D L D P E T V D D A F A R A F Q V W S D V T P L R F S R I H D G E A D I M I N F G R W E H G D G Y P F D G K D G L L A H A F A P G T G V G G D S H F D D D E L W T L G E G Q V V R V K Y G N A D G E Y C K F P F L F N G K E Y N S C T D T G R S D G F L W C S T T Y N F E K D G K Y G F C P H E A L F T M G G N A E G Q P C K F P F R F Q G T S Y D S C T T E G R T D G Y R W C G T T E D Y D R D K K Y G F C P E T A M S T V G G N S E G A P C V F P F T F L G N K Y E S C T S A G R S D G K M W C A T T A N Y D D D R K W G F C P D Q G Y S L F L V A A H E F G H A M G L E H S Q D P G A L M A P I Y T Y T K N F R L S Q D D I K G I Q E L Y G A S P D I D L G T G P T P T L G P V T P E I C K Q D I V F D G I A Q I R G E I F F F K D R F I W R T V T P R D K P M G P L L V A T F W P E L P E K I D A V Y E A P Q E E K A V F F A G N E Y W I Y S A S T L E R G Y P K P L T S L G L P P D V Q R V D A A F N W S K N K K T Y I F A G D K F W R Y N E V K K K M D P G F P K L I A D A W N A I P D N L D A V V D L Q G G G H S Y F F K G A Y Y L K L E N Q S L K S V K F G S I K S D W L G C

Accession
P08253-1
Protein length

Full Length

Animal free

No

Nature

Recombinant

Species

Human

Concentration
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Storage buffer

pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Specifications

Form

Liquid

Additional notes

>=95% Purity by HPLC

General info

Function

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.

Sequence similarities

Belongs to the peptidase M10A family.

Post-translational modifications

Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.

Subcellular localisation

Nucleus, Mitochondrion

Storage

Shipped at conditions

Dry Ice

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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