Recombinant human MMP2 protein (Active)
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Recombinant human MMP2 protein (Active) is a Human Full Length protein in the 110 to 660 aa range with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for Functional studies. The predicted molecular weight of ab280347 protein is 63 kDa.
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View Alternative Names
CLG4A, MMP2, 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, TBE-1, MMP-2
- FuncS
Supplier Data
Functional Studies - Recombinant human MMP2 protein (Active) (AB280347)
Biological activity of ab280347. Passed with a specific activity above >1,100 pmol/min/μg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot : GR3379499-4
- Mass Spec
Supplier Data
Mass Spectrometry - Recombinant human MMP2 protein (Active) (AB280347)
Mass determination by ESI-TOF.
Predicted MW is 62116.46 Da. (+/- 10 Da by ESI-TOF). Observed MW is 26116.57 Da. Additional masses are due to residual O-glycans.
- HPLC
Supplier Data
HPLC - Recombinant human MMP2 protein (Active) (AB280347)
HPLC analysis of ab280347.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant human MMP2 protein (Active) (AB280347)
SDS-Page anaylsis of ab280347.
Reactivity data
Product details
The ab280347 MMP2 protein is sourced from HEK293 cells and can be used as a positive control in SDS-PAGE, mass spectrometry and HPLC.
Check out our protein gel staining guide for SDS-PAGE here
Premium Bioactive Protein range
The ab280347 MMP2 protein is part of the premium bioactive protein range which are ideal for preclinical cell culture and functional studies. These recombinant proteins are of the highest activity, purity, and consistency, meeting rigorous biophysical characterization.
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Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Matrix metalloproteinase-2 is mainly involved in the degradation of type IV and V collagens gelatin and fibronectin. As part of the metalloproteinase family it works alongside other MMPs to maintain tissue homeostasis and repair. MMP-2 forms part of a complex network that ensures the timely degradation of matrix components balancing synthesis and breakdown. It remains regulated by tissue inhibitors of metalloproteinases (TIMPs) preventing excessive degradation that could lead to tissue damage.
Pathways
MMP-2 plays a significant role within the extracellular matrix (ECM) remodeling and angiogenesis pathways. It interacts with various proteins including integrins and TIMP-2 to modulate cellular behaviors such as migration and invasion. MMP-2 contributes to processes like wound healing and embryonic development through its involvement in ECM degradation and new tissue formation.
Specifications
Form
Liquid
Additional notes
>=95% Purity by HPLC
General info
Function
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.. PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.. Isoform 2. Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
Sequence similarities
Belongs to the peptidase M10A family.
Post-translational modifications
Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.. The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
Subcellular localisation
Nucleus
Target data
Complementary Products
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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