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Recombinant human MMP3 protein (Active) is a Human Full Length protein, in the 101 to 477 aa range, expressed in Escherichia coli, with >=98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, WB, FuncS.

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Key facts

Purity

>=98% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, HPLC, WB, FuncS

Biologically active

Yes

Amino acid sequence

M R T F P G I P K W R K T H L T Y R I V N Y T P D L P K D A V D S A V E K A L K V W E E V T P L T F S R L Y E G E A D I M I S F A V R E H G D F Y P F D G P G N V L A H A Y A P G P G I N G D A H F D D D E Q W T K D T T G T N L F L V A A H E I G H S L G L F H S A N T E A L M Y P L Y H S L T D L T R F R L S Q D D I N G I Q S L Y G P P P D S P E T P L V P T E P V P P E P G T P A N C D P A L S F D A V S T L R G E I L I F K D R H F W R K S L R K L E P E L H L I S S F W P S L P S G V D A A Y E V T S K D L V F I F K G N Q F W A I R G N E V R A G Y P R G I H T L G F P P T V R K I D A A I S D K E K N K T Y F F V E D K Y W R F D E K R N S M E P G F P K Q I A E D F P G I D S K I D A V F E E F G F F Y F F T G S S Q L E F D P N A K K V T H T L K S N S W L N C

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Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

HPLC

Reactivity

Reacts

Dilution info

-

Notes

-

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

Ab96555 can be used as a WB positive control in conjunction with ab18898.

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

Alternative names

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Recombinant human MMP3 protein (Active) is a Human Full Length protein, in the 101 to 477 aa range, expressed in Escherichia coli, with >=98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, WB, FuncS.

Alternative names

Key facts

Purity

>=98% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Applications

SDS-PAGE, HPLC, WB, FuncS

Biological activity

ab96555 activity was measured by its ability to cleave a chromogenic peptide MMP3 substrate at room temperature.

At a MMP3 concentration of 2.5 μg/ml, 50% cleavage was achieved at an incubation time of approximately 75 minutes.

Accession
P08254-1
Animal free

No

Species

Human

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex.

Concentration
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Storage buffer

Constituents: 0.164% Sodium phosphate, 0.1% CHAPS, 0.002% Ethylenediaminetetraacetic acid, disodium, dihydrate, 0.00111% Calcium chloride

Sequence info

Amino acid sequence

M R T F P G I P K W R K T H L T Y R I V N Y T P D L P K D A V D S A V E K A L K V W E E V T P L T F S R L Y E G E A D I M I S F A V R E H G D F Y P F D G P G N V L A H A Y A P G P G I N G D A H F D D D E Q W T K D T T G T N L F L V A A H E I G H S L G L F H S A N T E A L M Y P L Y H S L T D L T R F R L S Q D D I N G I Q S L Y G P P P D S P E T P L V P T E P V P P E P G T P A N C D P A L S F D A V S T L R G E I L I F K D R H F W R K S L R K L E P E L H L I S S F W P S L P S G V D A A Y E V T S K D L V F I F K G N Q F W A I R G N E V R A G Y P R G I H T L G F P P T V R K I D A A I S D K E K N K T Y F F V E D K Y W R F D E K R N S M E P G F P K Q I A E D F P G I D S K I D A V F E E F G F F Y F F T G S S Q L E F D P N A K K V T H T L K S N S W L N C

Accession

P08254

Protein length

Full Length

Predicted molecular weight

42.8 kDa

Amino acids

101 to 477

Nature

Recombinant

Specifications

Form

Lyophilized

Additional notes

>=98% HPLC analyses.

General info

Function

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

Sequence similarities

Belongs to the peptidase M10A family.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA), Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C.

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Supplementary info

Biological summary

MMP3 contributes to multiple physiological and pathological processes. It supports the remodeling of connective tissues and assists in normal developmental processes. MMP3 can work as part of a complex with other MMP proteins which can enhance or inhibit its activity. The balance of MMPs including MMP3 regulates the extracellular matrix turnover and is important for maintaining tissue homeostasis.

Mechanical summary

MMP3 also known as stromelysin-1 is a member of the matrix metalloproteinase (MMP) family. These proteins are zinc-dependent endopeptidases. MMP3 specifically has a molecular weight of approximately 54 kDa and is responsible for degrading extracellular matrix components such as collagen fibronectin and laminin. MMP3 is expressed in various tissues including fibroblasts chondrocytes and endothelial cells. It plays a significant role in tissue remodeling wound healing and potentially aids in the breakdown of cellular matrices.

Pathway

MMP3 participates in pathways involved in tissue remodeling and inflammatory responses. It is part of cellular pathways regulated by cytokines like interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha). These pathways link MMP3 to other proteins such as MMP1 and MMP9 which perform overlapping and distinct roles in matrix degradation during inflammation and tissue repair.

Disease

MMP3 is implicated in conditions like arthritis and cardiovascular diseases. MMP3 contributes to cartilage degradation in rheumatoid arthritis and is associated with the breakdown of vascular structures in atherosclerosis. Proteins like MMP9 and MMP13 often interact with MMP3 in these pathological conditions forming a network that exacerbates tissue damage and disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Recombinant human MMP3 protein (Active) (ab96555), expandable thumbnail

    Western blot - Recombinant human MMP3 protein (Active) (ab96555)

    All lanes: Anti-MMP3 antibody (ab18898) at 1 µg/mL

    All lanes: Western blot - Recombinant human MMP3 protein (Active) (AB96555) at 0.1 µg

    Secondary

    All lanes: Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 3min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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