Recombinant human MMP3 protein (Active) is a Human Full Length protein, in the 101 to 477 aa range, expressed in Escherichia coli, with >=98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, WB, FuncS.
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- Science advances 9:eadf65372023Matrix metalloproteinase-3 (MMP-3)-mediated gene therapy for glaucoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeffrey O'Callaghan,Conor Delaney,Merissa O'Connor,Joseph van Batenburg-Sherwood,Martin Schicht,Elke Lütjen-Drecoll,Natalie Hudson,Sorcha Ni Dhubhghaill,Peter Humphries,Chris Stanley,Annahita Keravala,Thomas Chalberg,Matthew S Lawrence,Matthew CampbellPubMed 37075118
- Neurobiology of disease 148:1051502020A new role for matrix metalloproteinase-3 in the NGF metabolic pathway: Proteolysis of mature NGF and sex-specific differences in the continuum of Alzheimer's pathology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRowan Pentz,M Florencia Iulita,Maya Mikutra-Cencora,Adriana Ducatenzeiler,David A Bennett,A Claudio CuelloPubMed 33130223
- Oncogene 39:204-2182019Notch3 promotes prostate cancer-induced bone lesion development via MMP-3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSourik S Ganguly,Galen Hostetter,Lin Tang,Sander B Frank,Kathylynn Saboda,Rohit Mehra,Lisha Wang,Xiaohong Li,Evan T Keller,Cindy K MirantiPubMed 31467432
- Human molecular genetics 26:1230-12462017Therapeutic potential of AAV-mediated MMP-3 secretion from corneal endothelium in treating glaucoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeffrey O'Callaghan,Darragh E Crosbie,Paul S Cassidy,Joseph M Sherwood,Cassandra Flügel-Koch,Elke Lütjen-Drecoll,Marian M Humphries,Ester Reina-Torres,Deborah Wallace,Anna-Sophia Kiang,Matthew Campbell,W Daniel Stamer,Darryl R Overby,Colm O'Brien,Lawrence C S Tam,Peter HumphriesPubMed 28158775
Key facts
- Purity
- >=98% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, HPLC, WB, FuncS
- Biologically active
- Yes
Amino acid sequence
M R T F P G I P K W R K T H L T Y R I V N Y T P D L P K D A V D S A V E K A L K V W E E V T P L T F S R L Y E G E A D I M I S F A V R E H G D F Y P F D G P G N V L A H A Y A P G P G I N G D A H F D D D E Q W T K D T T G T N L F L V A A H E I G H S L G L F H S A N T E A L M Y P L Y H S L T D L T R F R L S Q D D I N G I Q S L Y G P P P D S P E T P L V P T E P V P P E P G T P A N C D P A L S F D A V S T L R G E I L I F K D R H F W R K S L R K L E P E L H L I S S F W P S L P S G V D A A Y E V T S K D L V F I F K G N Q F W A I R G N E V R A G Y P R G I H T L G F P P T V R K I D A A I S D K E K N K T Y F F V E D K Y W R F D E K R N S M E P G F P K Q I A E D F P G I D S K I D A V F E E F G F F Y F F T G S S Q L E F D P N A K K V T H T L K S N S W L N C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab96555 can be used as a WB positive control in conjunction with ab18898. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Metalloproteinase with a rather broad substrate specificity that can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates different molecules including growth factors, plasminogen or other matrix metalloproteinases such as MMP9 (PubMed:11029580, PubMed:1371271). Once released into the extracellular matrix (ECM), the inactive pro-enzyme is activated by the plasmin cascade signaling pathway (PubMed:2383557). Acts also intracellularly (PubMed:22265821). For example, in dopaminergic neurons, gets activated by the serine protease HTRA2 upon stress and plays a pivotal role in DA neuronal degeneration by mediating microglial activation and alpha-synuclein/SNCA cleavage (PubMed:21330369). In addition, plays a role in immune response and possesses antiviral activity against various viruses such as vesicular stomatitis virus, influenza A virus (H1N1) and human herpes virus 1 (PubMed:35940311). Mechanistically, translocates from the cytoplasm into the cell nucleus upon virus infection to influence NF-kappa-B activities (PubMed:35940311).
Alternative names
STMY1, MMP3, Stromelysin-1, SL-1, Matrix metalloproteinase-3, Transin-1, MMP-3
Recommended products
Recombinant human MMP3 protein (Active) is a Human Full Length protein, in the 101 to 477 aa range, expressed in Escherichia coli, with >=98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, WB, FuncS.
Key facts
- Purity
- >=98% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, HPLC, WB, FuncS
- Biologically active
- Yes
- Biological activity
- ab96555 activity was measured by its ability to cleave a chromogenic peptide MMP3 substrate at room temperature.
At a MMP3 concentration of 2.5 μg/ml, 50% cleavage was achieved at an incubation time of approximately 75 minutes.
- Accession
- P08254-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute in water
- Concentration
- Storage buffer
Constituents: 0.164% Sodium phosphate, 0.1% 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate, 0.002% Ethylenediaminetetraacetic acid, disodium, dihydrate, 0.00111% Calcium chloride
Sequence info
Amino acid sequence
- M R T F P G I P K W R K T H L T Y R I V N Y T P D L P K D A V D S A V E K A L K V W E E V T P L T F S R L Y E G E A D I M I S F A V R E H G D F Y P F D G P G N V L A H A Y A P G P G I N G D A H F D D D E Q W T K D T T G T N L F L V A A H E I G H S L G L F H S A N T E A L M Y P L Y H S L T D L T R F R L S Q D D I N G I Q S L Y G P P P D S P E T P L V P T E P V P P E P G T P A N C D P A L S F D A V S T L R G E I L I F K D R H F W R K S L R K L E P E L H L I S S F W P S L P S G V D A A Y E V T S K D L V F I F K G N Q F W A I R G N E V R A G Y P R G I H T L G F P P T V R K I D A A I S D K E K N K T Y F F V E D K Y W R F D E K R N S M E P G F P K Q I A E D F P G I D S K I D A V F E E F G F F Y F F T G S S Q L E F D P N A K K V T H T L K S N S W L N C
- Accession
- P08254
- Protein length
- Full Length
- Predicted molecular weight
- 42.8 kDa
- Amino acids
- 101 to 477
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
>=98% HPLC analyses.
General info
- Function
Metalloproteinase with a rather broad substrate specificity that can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates different molecules including growth factors, plasminogen or other matrix metalloproteinases such as MMP9 (PubMed:11029580, PubMed:1371271). Once released into the extracellular matrix (ECM), the inactive pro-enzyme is activated by the plasmin cascade signaling pathway (PubMed:2383557). Acts also intracellularly (PubMed:22265821). For example, in dopaminergic neurons, gets activated by the serine protease HTRA2 upon stress and plays a pivotal role in DA neuronal degeneration by mediating microglial activation and alpha-synuclein/SNCA cleavage (PubMed:21330369). In addition, plays a role in immune response and possesses antiviral activity against various viruses such as vesicular stomatitis virus, influenza A virus (H1N1) and human herpes virus 1 (PubMed:35940311). Mechanistically, translocates from the cytoplasm into the cell nucleus upon virus infection to influence NF-kappa-B activities (PubMed:35940311).
- Sequence similarities
Belongs to the peptidase M10A family.
- Post-translational modifications
Directly cleaved by HTRA2 to produce active form.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA), Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MMP3 also known as stromelysin-1 is a member of the matrix metalloproteinase (MMP) family. These proteins are zinc-dependent endopeptidases. MMP3 specifically has a molecular weight of approximately 54 kDa and is responsible for degrading extracellular matrix components such as collagen fibronectin and laminin. MMP3 is expressed in various tissues including fibroblasts chondrocytes and endothelial cells. It plays a significant role in tissue remodeling wound healing and potentially aids in the breakdown of cellular matrices.
- Biological function summary
MMP3 contributes to multiple physiological and pathological processes. It supports the remodeling of connective tissues and assists in normal developmental processes. MMP3 can work as part of a complex with other MMP proteins which can enhance or inhibit its activity. The balance of MMPs including MMP3 regulates the extracellular matrix turnover and is important for maintaining tissue homeostasis.
- Pathways
MMP3 participates in pathways involved in tissue remodeling and inflammatory responses. It is part of cellular pathways regulated by cytokines like interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha). These pathways link MMP3 to other proteins such as MMP1 and MMP9 which perform overlapping and distinct roles in matrix degradation during inflammation and tissue repair.
- Associated diseases and disorders
MMP3 is implicated in conditions like arthritis and cardiovascular diseases. MMP3 contributes to cartilage degradation in rheumatoid arthritis and is associated with the breakdown of vascular structures in atherosclerosis. Proteins like MMP9 and MMP13 often interact with MMP3 in these pathological conditions forming a network that exacerbates tissue damage and disease progression.
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Western blot - Recombinant human MMP3 protein (Active) (ab96555)
All lanes: Anti-MMP3 antibody (ab18898) at 1 µg/mL
All lanes: Western blot - Recombinant human MMP3 protein (Active) (ab96555) at 0.1 µg
SecondaryAll lanes: Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 3min
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