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Recombinant human MMP3 protein (Active) is a Human Full Length protein, in the 101 to 477 aa range, expressed in Escherichia coli, with >=98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, WB, FuncS.
Alternative names=Stromelysin-1, SL-1, Matrix metalloproteinase-3, Transin-1, MMP-3, STMY1, MMP3
>=98% SDS-PAGE
< 1 EU/µg
Escherichia coli
Tag free
SDS-PAGE, HPLC, WB, FuncS
Yes
M R T F P G I P K W R K T H L T Y R I V N Y T P D L P K D A V D S A V E K A L K V W E E V T P L T F S R L Y E G E A D I M I S F A V R E H G D F Y P F D G P G N V L A H A Y A P G P G I N G D A H F D D D E Q W T K D T T G T N L F L V A A H E I G H S L G L F H S A N T E A L M Y P L Y H S L T D L T R F R L S Q D D I N G I Q S L Y G P P P D S P E T P L V P T E P V P P E P G T P A N C D P A L S F D A V S T L R G E I L I F K D R H F W R K S L R K L E P E L H L I S S F W P S L P S G V D A A Y E V T S K D L V F I F K G N Q F W A I R G N E V R A G Y P R G I H T L G F P P T V R K I D A A I S D K E K N K T Y F F V E D K Y W R F D E K R N S M E P G F P K Q I A E D F P G I D S K I D A V F E E F G F F Y F F T G S S Q L E F D P N A K K V T H T L K S N S W L N C
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes Ab96555 can be used as a WB positive control in conjunction with ab18898. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Recombinant human MMP3 protein (Active) is a Human Full Length protein, in the 101 to 477 aa range, expressed in Escherichia coli, with >=98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, WB, FuncS.
Alternative names=Stromelysin-1, SL-1, Matrix metalloproteinase-3, Transin-1, MMP-3, STMY1, MMP3
>=98% SDS-PAGE
< 1 EU/µg
Escherichia coli
Tag free
SDS-PAGE, HPLC, WB, FuncS
Yes
ab96555 activity was measured by its ability to cleave a chromogenic peptide MMP3 substrate at room temperature.
At a MMP3 concentration of 2.5 μg/ml, 50% cleavage was achieved at an incubation time of approximately 75 minutes.
42.8 kDa
101 to 477
M R T F P G I P K W R K T H L T Y R I V N Y T P D L P K D A V D S A V E K A L K V W E E V T P L T F S R L Y E G E A D I M I S F A V R E H G D F Y P F D G P G N V L A H A Y A P G P G I N G D A H F D D D E Q W T K D T T G T N L F L V A A H E I G H S L G L F H S A N T E A L M Y P L Y H S L T D L T R F R L S Q D D I N G I Q S L Y G P P P D S P E T P L V P T E P V P P E P G T P A N C D P A L S F D A V S T L R G E I L I F K D R H F W R K S L R K L E P E L H L I S S F W P S L P S G V D A A Y E V T S K D L V F I F K G N Q F W A I R G N E V R A G Y P R G I H T L G F P P T V R K I D A A I S D K E K N K T Y F F V E D K Y W R F D E K R N S M E P G F P K Q I A E D F P G I D S K I D A V F E E F G F F Y F F T G S S Q L E F D P N A K K V T H T L K S N S W L N C
Full Length
No
Recombinant
Human
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex.
Constituents: 0.164% Sodium phosphate, 0.1% CHAPS, 0.002% Ethylenediaminetetraacetic acid, disodium, dihydrate, 0.00111% Calcium chloride
Lyophilized
>=98% HPLC analyses.
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
Belongs to the peptidase M10A family.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA), Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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