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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Recombinant human MMP3 protein (Active) is a Human Full Length protein, in the 101 to 477 aa range, expressed in Escherichia coli, with >=98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, WB, FuncS.
>=98% SDS-PAGE
< 1 EU/µg
Escherichia coli
Tag free
SDS-PAGE, HPLC, WB, FuncS
Yes
M R T F P G I P K W R K T H L T Y R I V N Y T P D L P K D A V D S A V E K A L K V W E E V T P L T F S R L Y E G E A D I M I S F A V R E H G D F Y P F D G P G N V L A H A Y A P G P G I N G D A H F D D D E Q W T K D T T G T N L F L V A A H E I G H S L G L F H S A N T E A L M Y P L Y H S L T D L T R F R L S Q D D I N G I Q S L Y G P P P D S P E T P L V P T E P V P P E P G T P A N C D P A L S F D A V S T L R G E I L I F K D R H F W R K S L R K L E P E L H L I S S F W P S L P S G V D A A Y E V T S K D L V F I F K G N Q F W A I R G N E V R A G Y P R G I H T L G F P P T V R K I D A A I S D K E K N K T Y F F V E D K Y W R F D E K R N S M E P G F P K Q I A E D F P G I D S K I D A V F E E F G F F Y F F T G S S Q L E F D P N A K K V T H T L K S N S W L N C
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes Ab96555 can be used as a WB positive control in conjunction with ab18898. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
Stromelysin-1, SL-1, Matrix metalloproteinase-3, Transin-1, MMP-3, STMY1, MMP3
Recombinant human MMP3 protein (Active) is a Human Full Length protein, in the 101 to 477 aa range, expressed in Escherichia coli, with >=98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, WB, FuncS.
Stromelysin-1, SL-1, Matrix metalloproteinase-3, Transin-1, MMP-3, STMY1, MMP3
>=98% SDS-PAGE
< 1 EU/µg
Escherichia coli
Tag free
SDS-PAGE, HPLC, WB, FuncS
Yes
ab96555 activity was measured by its ability to cleave a chromogenic peptide MMP3 substrate at room temperature.
At a MMP3 concentration of 2.5 μg/ml, 50% cleavage was achieved at an incubation time of approximately 75 minutes.
No
Human
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex.
Constituents: 0.164% Sodium phosphate, 0.1% CHAPS, 0.002% Ethylenediaminetetraacetic acid, disodium, dihydrate, 0.00111% Calcium chloride
M R T F P G I P K W R K T H L T Y R I V N Y T P D L P K D A V D S A V E K A L K V W E E V T P L T F S R L Y E G E A D I M I S F A V R E H G D F Y P F D G P G N V L A H A Y A P G P G I N G D A H F D D D E Q W T K D T T G T N L F L V A A H E I G H S L G L F H S A N T E A L M Y P L Y H S L T D L T R F R L S Q D D I N G I Q S L Y G P P P D S P E T P L V P T E P V P P E P G T P A N C D P A L S F D A V S T L R G E I L I F K D R H F W R K S L R K L E P E L H L I S S F W P S L P S G V D A A Y E V T S K D L V F I F K G N Q F W A I R G N E V R A G Y P R G I H T L G F P P T V R K I D A A I S D K E K N K T Y F F V E D K Y W R F D E K R N S M E P G F P K Q I A E D F P G I D S K I D A V F E E F G F F Y F F T G S S Q L E F D P N A K K V T H T L K S N S W L N C
Full Length
42.8 kDa
101 to 477
Recombinant
Lyophilized
>=98% HPLC analyses.
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
Belongs to the peptidase M10A family.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA), Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
MMP3 contributes to multiple physiological and pathological processes. It supports the remodeling of connective tissues and assists in normal developmental processes. MMP3 can work as part of a complex with other MMP proteins which can enhance or inhibit its activity. The balance of MMPs including MMP3 regulates the extracellular matrix turnover and is important for maintaining tissue homeostasis.
MMP3 also known as stromelysin-1 is a member of the matrix metalloproteinase (MMP) family. These proteins are zinc-dependent endopeptidases. MMP3 specifically has a molecular weight of approximately 54 kDa and is responsible for degrading extracellular matrix components such as collagen fibronectin and laminin. MMP3 is expressed in various tissues including fibroblasts chondrocytes and endothelial cells. It plays a significant role in tissue remodeling wound healing and potentially aids in the breakdown of cellular matrices.
MMP3 participates in pathways involved in tissue remodeling and inflammatory responses. It is part of cellular pathways regulated by cytokines like interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha). These pathways link MMP3 to other proteins such as MMP1 and MMP9 which perform overlapping and distinct roles in matrix degradation during inflammation and tissue repair.
MMP3 is implicated in conditions like arthritis and cardiovascular diseases. MMP3 contributes to cartilage degradation in rheumatoid arthritis and is associated with the breakdown of vascular structures in atherosclerosis. Proteins like MMP9 and MMP13 often interact with MMP3 in these pathological conditions forming a network that exacerbates tissue damage and disease progression.
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Full details and terms and conditions can be found here:
Terms & Conditions.
All lanes: Anti-MMP3 antibody (ab18898) at 1 µg/mL
All lanes: Western blot - Recombinant human MMP3 protein (Active) (AB96555) at 0.1 µg
All lanes: Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 3min
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