Recombinant human MMP9 (mutated Q279R) protein is a Human Full Length protein, in the 20 to 707 aa range, expressed in HEK 293, with >90% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
A P R Q R Q S T L V L F P G D L R T N L T D R Q L A E E Y L Y R Y G Y T R V A E M R G E S K S L G P A L L L L Q K Q L S L P E T G E L D S A T L K A M R T P R C G V P D L G R F Q T F E G D L K W H H H N I T Y W I Q N Y S E D L P R A V I D D A F A R A F A L W S A V T P L T F T R V Y S R D A D I V I Q F G V A E H G D G Y P F D G K D G L L A H A F P P G P G I Q G D A H F D D D E L W S L G K G V V V P T R F G N A D G A A C H F P F I F E G R S Y S A C T T D G R S D G L P W C S T T A N Y D T D D R F G F C P S E R L Y T R D G N A D G K P C Q F P F I F Q G Q S Y S A C T T D G R S D G Y R W C A T T A N Y D R D K L F G F C P T R A D S T V M G G N S A G E L C V F P F T F L G K E Y S T C T S E G R G D G R L W C A T T S N F D S D K K W G F C P D Q G Y S L F L V A A H E F G H A L G L D H S S V P E A L M Y P M Y R F T E G P P L H K D D V N G I R H L Y G P R P E P E P R P P T T T T P Q P T A P P T V C P T G P P T V H P S E R P T A G P T G P P S A G P T G P P T A G P S T A T T V P L S P V D D A C N V N I F D A I A E I G N Q L Y L F K D G K Y W R F S E G R G S R P Q G P F L I A D K W P A L P R K L D S V F E E R L S K K L F F F S G R Q V W V Y T G A S V L G P R R L D K L G L G A D V A Q V T G A L R S G R G K M L L F S G R R L W R F D V K A Q M V D P R S A S E V D R M F P G V P L D T H D V F Q Y R E K A Y F C Q D R F Y W R V S S R S E L N Q V D Q V G Y V T Y D I L Q C P E D H H H H H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration (PubMed:12879005, PubMed:1480034, PubMed:2551898). Could play a role in bone osteoclastic resorption (By similarity). Cleaves KiSS1 at a Gly-|-Leu bond (PubMed:12879005). Cleaves NINJ1 to generate the Secreted ninjurin-1 form (PubMed:32883094). Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (PubMed:1480034). Degrades fibronectin but not laminin or Pz-peptide.
Alternative names
CLG4B, MMP9, Matrix metalloproteinase-9, MMP-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB
Recommended products
Recombinant human MMP9 (mutated Q279R) protein is a Human Full Length protein, in the 20 to 707 aa range, expressed in HEK 293, with >90% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific Activity: ≥ 2500 pmole/min/μg
Activation: This is a proform enzyme and requires activation prior to testing activity. Dilute the enzyme to 100 ng/ml in a solution containing 50 mM HEPES, pH 7.4, 10 mM CaCl2, 0.05% Brij-35, and 1 mM APMA (amino-phenyl mercuric acetate). Incubate at 37°C for 2 hours.
Assay conditions: Reaction mixture with 10 μM MMP substrate. Incubate for 30 minutes at room temperature. Fluorescence intensity is measured at Ex328/Em393. - Accession
- P14780-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Preservative: 1.36% Imidazole
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- A P R Q R Q S T L V L F P G D L R T N L T D R Q L A E E Y L Y R Y G Y T R V A E M R G E S K S L G P A L L L L Q K Q L S L P E T G E L D S A T L K A M R T P R C G V P D L G R F Q T F E G D L K W H H H N I T Y W I Q N Y S E D L P R A V I D D A F A R A F A L W S A V T P L T F T R V Y S R D A D I V I Q F G V A E H G D G Y P F D G K D G L L A H A F P P G P G I Q G D A H F D D D E L W S L G K G V V V P T R F G N A D G A A C H F P F I F E G R S Y S A C T T D G R S D G L P W C S T T A N Y D T D D R F G F C P S E R L Y T R D G N A D G K P C Q F P F I F Q G Q S Y S A C T T D G R S D G Y R W C A T T A N Y D R D K L F G F C P T R A D S T V M G G N S A G E L C V F P F T F L G K E Y S T C T S E G R G D G R L W C A T T S N F D S D K K W G F C P D Q G Y S L F L V A A H E F G H A L G L D H S S V P E A L M Y P M Y R F T E G P P L H K D D V N G I R H L Y G P R P E P E P R P P T T T T P Q P T A P P T V C P T G P P T V H P S E R P T A G P T G P P S A G P T G P P T A G P S T A T T V P L S P V D D A C N V N I F D A I A E I G N Q L Y L F K D G K Y W R F S E G R G S R P Q G P F L I A D K W P A L P R K L D S V F E E R L S K K L F F F S G R Q V W V Y T G A S V L G P R R L D K L G L G A D V A Q V T G A L R S G R G K M L L F S G R R L W R F D V K A Q M V D P R S A S E V D R M F P G V P L D T H D V F Q Y R E K A Y F C Q D R F Y W R V S S R S E L N Q V D Q V G Y V T Y D I L Q C P E D H H H H H H H H H H
- Accession
- P14780
- Protein length
- Full Length
- Predicted molecular weight
- 78 kDa
- Amino acids
- 20 to 707
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
General info
- Function
Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration (PubMed:12879005, PubMed:1480034, PubMed:2551898). Could play a role in bone osteoclastic resorption (By similarity). Cleaves KiSS1 at a Gly-|-Leu bond (PubMed:12879005). Cleaves NINJ1 to generate the Secreted ninjurin-1 form (PubMed:32883094). Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (PubMed:1480034). Degrades fibronectin but not laminin or Pz-peptide.
- Sequence similarities
Belongs to the peptidase M10A family.
- Post-translational modifications
Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MMP-9 protein also known as matrix metalloproteinase-9 or gelatinase B functions as an enzyme involved in the breakdown of extracellular matrix proteins. It consists of a zinc ion at its active site and facilitates the degradation of type IV and V collagens fibronectin and elastin. MMP-9 has a molecular weight of approximately 92 kDa and is expressed in a variety of tissues including the liver lungs and immune cells. This enzyme can exist in monomer and dimer forms with its activity regulated by tissue inhibitors of metalloproteinases (TIMPs).
- Biological function summary
Matrix metalloproteinase-9 profoundly influences tissue remodeling inflammation and angiogenesis. It does not form part of a larger protein complex but interacts closely with substrates in the extracellular matrix. MMP-9 plays important roles in processes such as embryogenesis reproduction and wound healing through the remodeling of surrounding tissues. The MMP-9 protein is often measured using assays like ELISA or analyzed via techniques such as Western blotting to determine its expression levels and activity in different biological contexts.
- Pathways
MMP-9 participates in the matrix metalloproteinase pathway and the NF-kB signaling pathway. These pathways are essential in orchestrating processes such as tissue remodeling and inflammatory responses. MMP-9 acts alongside other matrix metalloproteinases including MMP-2 and is regulated by factors like cytokines growth factors and hormones ensuring the precision of extracellular matrix degradation during physiological processes.
- Associated diseases and disorders
Matrix metalloproteinase-9 has associations with cancer invasion and metastasis as well as chronic inflammatory diseases like rheumatoid arthritis. In cancer upregulated MMP-9 expression aids tumor cells in breaching the basement membrane facilitating metastasis. The disorder-associated pathways often involve interactions with proteins like vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-beta). Understanding MMP-9's role in these conditions can contribute to developing targeted therapies that inhibit its activity potentially mitigating disease progression.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Functional Studies - Recombinant human MMP9 (mutated Q279R) protein (ab198459)
Specific activity of activated ab198459.
SDS-PAGE - Recombinant human MMP9 (mutated Q279R) protein (ab198459)
4-20% SDS-PAGE analysis of ab198459 with Coomassie staining.
Lane 1: 6.3 μg ab198459
Lane 2: Protein marker
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com