Recombinant Human MMP9 protein (Proenzyme)

• sELISA

Unknown

Sandwich ELISA - Recombinant Human MMP9 protein (Proenzyme) (AB82955)

Standard curve for MMP9 (Analyte : ab82955); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [SB15c] to MMP9 (ab51203) at 5μg/ml and Detector Antibody Rabbit polyclonal to MMP9 - Hinge region (ab38902) at 0.5μg/ml.

• WB

Unknown

Western blot - Recombinant Human MMP9 protein (Proenzyme) (AB82955)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab73734 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406,

All lanes:

Western blot - Anti-MMP9 antibody (<a href='/en-us/products/primary-antibodies/mmp9-antibody-ab73734'>ab73734</a>) at 1 µg

All lanes:

Western blot - Recombinant Human MMP9 protein (Proenzyme) (ab82955) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 78 kDa

true

Exposure time: 2min

• WB

Unknown

Western blot - Recombinant Human MMP9 protein (Proenzyme) (AB82955)

All lanes:

Western blot - Anti-MMP9 antibody (<a href='/en-us/products/primary-antibodies/mmp9-antibody-ab73734'>ab73734</a>) at 1 µg

All lanes:

Western blot - Recombinant Human MMP9 protein (Proenzyme) (ab82955) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 78 kDa

true

Exposure time: 2min

• WB

Unknown

Western blot - Recombinant Human MMP9 protein (Proenzyme) (AB82955)

Running buffer : MOPS. Conditions : Denatured/reduced. This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab76003 (rabbit-anti MMP9; 1.5 ug/mL) for 48 hours at 4°C. Before imaging, antibody binding was detected using infrared-labeled goat anti-rabbit (green) at 1 : 10,000 dilutions for 1 hour at room temperature.

All lanes:

Western blot - Anti-MMP9 antibody [EP1254] (<a href='/en-us/products/primary-antibodies/mmp9-antibody-ep1254-ab76003'>ab76003</a>) at 1.5 µg/mL

All lanes:

Western blot - Recombinant Human MMP9 protein (Proenzyme) (ab82955) at 0.1 µg

Secondary

All lanes:

Goat anti-rabbit at 1/10000 dilution

Predicted band size: 78 kDa

Observed band size: 89 kDa

false

• SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MMP9 protein (Proenzyme) (AB82955)

ab82955 on SDS-PAGE.