Recombinant Human MMP9 protein (Proenzyme) is a Human Fragment protein, in the 20 to 707 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, ELISA, WB.
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- International journal of molecular sciences 19:2018Overexpression of the Vitronectin V10 Subunit in Patients with Nonalcoholic Steatohepatitis: Implications for Noninvasive Diagnosis of NASH.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria Del Ben et. al.PubMed 29463024
- Biochimica et biophysica acta. Molecular basis of 1863:2808-28202017Biological impact of advanced glycation endproducts on estrogen receptor-positive MCF-7 breast cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSabine Matou-Nasri et. al.PubMed 28712835
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab82955 can be used as a WB positive control in conjunction with Anti-MMP9 antibody ab73734. |
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Target data
Function
Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration (PubMed:12879005, PubMed:1480034, PubMed:2551898). Could play a role in bone osteoclastic resorption (By similarity). Cleaves KiSS1 at a Gly-|-Leu bond (PubMed:12879005). Cleaves NINJ1 to generate the Secreted ninjurin-1 form (PubMed:32883094). Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (PubMed:1480034). Degrades fibronectin but not laminin or Pz-peptide.
Alternative names
CLG4B, MMP9, Matrix metalloproteinase-9, MMP-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB
Recommended products
Recombinant Human MMP9 protein (Proenzyme) is a Human Fragment protein, in the 20 to 707 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P14780-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.2
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- Accession
- P14780
- Protein length
- Fragment
- Amino acids
- 20 to 707
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration (PubMed:12879005, PubMed:1480034, PubMed:2551898). Could play a role in bone osteoclastic resorption (By similarity). Cleaves KiSS1 at a Gly-|-Leu bond (PubMed:12879005). Cleaves NINJ1 to generate the Secreted ninjurin-1 form (PubMed:32883094). Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (PubMed:1480034). Degrades fibronectin but not laminin or Pz-peptide.
- Sequence similarities
Belongs to the peptidase M10A family.
- Post-translational modifications
Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MMP-9 protein also known as matrix metalloproteinase-9 or gelatinase B functions as an enzyme involved in the breakdown of extracellular matrix proteins. It consists of a zinc ion at its active site and facilitates the degradation of type IV and V collagens fibronectin and elastin. MMP-9 has a molecular weight of approximately 92 kDa and is expressed in a variety of tissues including the liver lungs and immune cells. This enzyme can exist in monomer and dimer forms with its activity regulated by tissue inhibitors of metalloproteinases (TIMPs).
- Biological function summary
Matrix metalloproteinase-9 profoundly influences tissue remodeling inflammation and angiogenesis. It does not form part of a larger protein complex but interacts closely with substrates in the extracellular matrix. MMP-9 plays important roles in processes such as embryogenesis reproduction and wound healing through the remodeling of surrounding tissues. The MMP-9 protein is often measured using assays like ELISA or analyzed via techniques such as Western blotting to determine its expression levels and activity in different biological contexts.
- Pathways
MMP-9 participates in the matrix metalloproteinase pathway and the NF-kB signaling pathway. These pathways are essential in orchestrating processes such as tissue remodeling and inflammatory responses. MMP-9 acts alongside other matrix metalloproteinases including MMP-2 and is regulated by factors like cytokines growth factors and hormones ensuring the precision of extracellular matrix degradation during physiological processes.
- Associated diseases and disorders
Matrix metalloproteinase-9 has associations with cancer invasion and metastasis as well as chronic inflammatory diseases like rheumatoid arthritis. In cancer upregulated MMP-9 expression aids tumor cells in breaching the basement membrane facilitating metastasis. The disorder-associated pathways often involve interactions with proteins like vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-beta). Understanding MMP-9's role in these conditions can contribute to developing targeted therapies that inhibit its activity potentially mitigating disease progression.
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5 product images
Western blot - Recombinant Human MMP9 protein (Proenzyme) (ab82955)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with Anti-MMP9 antibody ab73734 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406,
All lanes: Western blot - Anti-MMP9 antibody (Anti-MMP9 antibody ab73734) at 1 µg
All lanes: Western blot - Recombinant Human MMP9 protein (Proenzyme) (ab82955) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 78 kDa
Exposure time: 2min
SDS-PAGE - Recombinant Human MMP9 protein (Proenzyme) (ab82955)
ab82955 on SDS-PAGE.
Western blot - Recombinant Human MMP9 protein (Proenzyme) (ab82955)
Running buffer: MOPS.
Conditions: Denatured/reduced.
This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with Anti-MMP9 antibody [EP1254] ab76003 (rabbit-anti MMP9; 1.5 ug/mL) for 48 hours at 4°C. Before imaging, antibody binding was detected using infrared-labeled goat anti-rabbit (green) at 1:10,000 dilutions for 1 hour at room temperature.All lanes: Western blot - Anti-MMP9 antibody [EP1254] (Anti-MMP9 antibody [EP1254] ab76003) at 1.5 µg/mL
All lanes: Western blot - Recombinant Human MMP9 protein (Proenzyme) (ab82955) at 0.1 µg
SecondaryAll lanes: Goat anti-rabbit at 1/10000 dilution
Predicted band size: 78 kDa
Observed band size: 89 kDa
Western blot - Recombinant Human MMP9 protein (Proenzyme) (ab82955)
All lanes: Western blot - Anti-MMP9 antibody (Anti-MMP9 antibody ab73734) at 1 µg
All lanes: Western blot - Recombinant Human MMP9 protein (Proenzyme) (ab82955) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 78 kDa
Exposure time: 2min
Sandwich ELISA - Recombinant Human MMP9 protein (Proenzyme) (ab82955)
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