Recombinant Human MMS2 protein is a Human Full Length protein, in the 2 to 145 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB.
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Key facts
- Purity
- >95% Densitometry
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Amino acid sequence
A V S T G V K V P R N F R L L E E L E E G Q K G V G D G T V S W G L E D D E D M T L T R W T G M I I G P P R T N Y E N R I Y S L K V E C G P K Y P E A P P S V R F V T K I N M N G I N N S S G M V D A R S I P V L A K W Q N S Y S I K V V L Q E L R R L M M S K E N M K L P Q P P E G Q T Y N N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Has no ubiquitin ligase activity on its own. The UBE2V2/UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.
Alternative names
MMS2, UEV2, UBE2V2, Ubiquitin-conjugating enzyme E2 variant 2, DDVit 1, Enterocyte differentiation-associated factor 1, Enterocyte differentiation-promoting factor 1, MMS2 homolog, Vitamin D3-inducible protein, EDAF-1, EDPF-1
Recommended products
Recombinant Human MMS2 protein is a Human Full Length protein, in the 2 to 145 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >95% Densitometry
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- Q15819-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
Sequence info
Amino acid sequence
- A V S T G V K V P R N F R L L E E L E E G Q K G V G D G T V S W G L E D D E D M T L T R W T G M I I G P P R T N Y E N R I Y S L K V E C G P K Y P E A P P S V R F V T K I N M N G I N N S S G M V D A R S I P V L A K W Q N S Y S I K V V L Q E L R R L M M S K E N M K L P Q P P E G Q T Y N N
- Accession
- Q15819
- Protein length
- Full Length
- Predicted molecular weight
- 18 kDa
- Amino acids
- 2 to 145
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Has no ubiquitin ligase activity on its own. The UBE2V2/UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.
- Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Ube2v2, MMS2
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MMS2 protein also known as UBE2V2 is a ubiquitin-conjugating enzyme variant found within cells. Unlike typical ubiquitin-conjugating enzymes MMS2 lacks the conserved active site cysteine. The MMS2 protein has a molecular mass of approximately 16 kDa and is expressed in a range of tissues including those of the human immune system. Functionally MMS2 partners with another enzyme Ubc13 to facilitate the assembly of Lys63-linked polyubiquitin chains necessary for DNA damage tolerance and repair processes.
- Biological function summary
MMS2 plays a critical role in cellular responses to DNA damage. It is part of the Ubc13-MMS2 complex which contributes to the post-replication repair pathway. This complex modulates the signaling and resolution of DNA double-strand breaks. MMS2 through its role in this complex impacts cellular mechanisms for maintaining genomic integrity and preventing genomic instability.
- Pathways
MMS2 takes part in the DNA damage response and NF-kappaB signaling pathways. In the process of DNA repair it works with proteins like Rad6 and Rad18 enabling cells to overcome stalled replication forks and promoting cell survival in response to DNA damage. In the NF-kappaB pathway MMS2 contributes to signaling cascades that activate immune responses and inflammation working alongside other proteins to mediate cellular responses.
- Associated diseases and disorders
MMS2 is linked to cancer and neurodegenerative diseases. Its role in DNA repair and signaling pathways connects it to tumor suppression with defects in MMS2 function potentially leading to increased susceptibility to cancer due to accumulated DNA damage. Additionally MMS2's interaction with proteins involved in neuroinflammation suggests a possible connection to neurodegenerative conditions such as Alzheimer’s disease where dysregulated inflammation and repair pathways contribute to disease pathology.
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1 product image
SDS-PAGE - Recombinant Human MMS2 protein (ab167920)
SDS-PAGE analysis of ab167920.
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