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AB204195

Recombinant Human MNAT1 protein

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Recombinant Human MNAT1 protein is a Human Full Length protein, in the 2 to 309 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

CAP35, MAT1, RNF66, MNAT1, CDK-activating kinase assembly factor MAT1, CDK7/cyclin-H assembly factor, Cyclin-G1-interacting protein, Menage a trois, RING finger protein 66, RING finger protein MAT1, p35, p36

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His-T7 tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P51948

Animal free

No

Carrier free

No

Species

Human

Storage buffer

Constituents: 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MASMTGGQQMGRGHHHHHHENLYFQGDDQGCPRCKTTKYRNPSLKLMVNVCGHTLCESCVDLLFVRGAGNCPECGTPLRKSNFRVQLFEDPTVDKEVEIRKKVLKIYNKREEDFPSLREYNDFLEEVEEIVFNLTNNVDLDNTKKKMEIYQKENKDVIQKNKLKLTREQEELEEALEVERQENEQRRLFIQKEEQLQQILKRKNKQAFLDELESSDLPVALLLAQHKDRSTQLEMQLEKPKPVKPVTFSTGIKMGQHISLAPIHKLEEALYEYQPLQIETYGPHVPELEMLGRLGYLNHVRAASPQDLAGGYTSSLACHRALQDAFSGLFWQPS","proteinLength":"Full Length","predictedMolecularWeight":"38.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":309,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P51948","tags":[{"tag":"His-T7","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein MNAT1 also known as menage à trois 1 functions as a component of the CDK-activating kinase complex. It weighs about 32 kilodaltons. MNAT1 plays a role in regulating cell cycle progression. It expresses widely in various tissues but shows higher levels in proliferating tissues due to its involvement in cell division. Its expression pattern suggests a significant function in maintaining proper cell cycle control.
Biological function summary

This protein serves as a regulatory subunit of the CDK7-cyclin H-MNAT1 complex. This complex forms the core of CDK-activating kinase which is essential for the activation of other cyclin-dependent kinases (CDKs). By doing so MNAT1 influences transcription regulation and DNA repair processes. As part of this complex MNAT1 modulates the activity of the proteins involved in a variety of cellular mechanisms. Through these activities MNAT1 helps in maintaining genomic integrity and proper cellular response to DNA damage.

Pathways

The activity of MNAT1 is involved in the cell cycle regulation pathway and the transcription regulation process. It plays a role in phosphorylating other CDKs directly influencing their activation and consequently cell cycle control. MNAT1 associates closely with proteins such as cyclin H and CDK7 within these pathways. This collaboration ensures that the essential checkpoints in the cell cycle are correctly regulated and that transcription processes are executed properly.

Irregularities in MNAT1 function can correlate with cancer development due to its central role in cell cycle regulation and genomic stability. Altered expression levels or mutations in MNAT1 can disrupt normal cell division leading to unchecked cell proliferation. Furthermore MNAT1 interacts with cyclin H and CDK7 which are also important in the proper regulation of cell cycle checkpoints and can also influence cancer pathways when dysfunctional. Hence MNAT1 is a potential target for therapeutic strategies aiming to control abnormal cell growth.
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Specifications

Form

Liquid

Additional notes

ab204195 was refolded using unique “temperature shift inclusion body refolding” technology and chromatographically purified.

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General info

Function

Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.
See full target information MNAT1
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Complementary Products

Primary Antibodies

AB169546

Anti-MNAT1 antibody [EPR10940]

RabMAb|Recombinant

Western blot - Anti-MNAT1 antibody [EPR10940] (AB169546)

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