Recombinant Human MOB4A protein is a Human Full Length protein, in the 1 to 216 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M S F L F G S R S S K T F K P K K N I P E G S H Q Y E L L K H A E A T L G S G N L R M A V M L P E G E D L N E W V A V N T V D F F N Q I N M L Y G T I T D F C T E E S C P V M S A G P K Y E Y H W A D G T N I K K P I K C S A P K Y I D Y L M T W V Q D Q L D D E T L F P S K I G V P F P K N F M S V A K T I L K R L F R V Y A H I Y H Q H F D P V I Q L Q E E A H L N T S F K H F I F F V Q E F N L I D R R E L A P L Q E L I E K L T S K D R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38L.
Alternative names
MOB4A, MOBKL1A, MOB1B, MOB kinase activator 1B, Mob1 homolog 1A, Mob1B, Mps one binder kinase activator-like 1A, Mob1A
Recommended products
Recombinant Human MOB4A protein is a Human Full Length protein, in the 1 to 216 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q7L9L4-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M S F L F G S R S S K T F K P K K N I P E G S H Q Y E L L K H A E A T L G S G N L R M A V M L P E G E D L N E W V A V N T V D F F N Q I N M L Y G T I T D F C T E E S C P V M S A G P K Y E Y H W A D G T N I K K P I K C S A P K Y I D Y L M T W V Q D Q L D D E T L F P S K I G V P F P K N F M S V A K T I L K R L F R V Y A H I Y H Q H F D P V I Q L Q E E A H L N T S F K H F I F F V Q E F N L I D R R E L A P L Q E L I E K L T S K D R
- Accession
- Q7L9L4
- Protein length
- Full Length
- Predicted molecular weight
- 27 kDa
- Amino acids
- 1 to 216
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab140717 is purified using conventional chromatography techniques.
General info
- Function
Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38L.
- Sequence similarities
Belongs to the MOB1/phocein family.
- Post-translational modifications
Phosphorylated by STK3/MST2 and STK4/MST1 and this phosphorylation enhances its binding to LATS1.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MOB4A also known by MOB kinase activator 4A stands as a significant player in cellular mechanisms. This protein weighs approximately 26 kDa and shows expression in many tissues across the human body notably in the brain and testis. It interacts with several other proteins through its peptide domains to control various cell processes. MOB4A plays a role in the regulation of cell proliferation and survival by modulating signaling pathways.
- Biological function summary
MOB4A interacts as a component of the large kinase complexes which play an essential role in cell cycle regulation and apoptosis. MOB4A's activity involves binding to certain serine-threonine kinases in the Hippo signaling pathway. By doing this it influences cellular growth control and prevents unchecked cell proliferation. The protein's ability to form complexes enhances its regulatory functions and impacts various cellular responses.
- Pathways
MOB4A participates actively in the Hippo and mTOR signaling pathways important pathways that regulate cell growth and survival. In the Hippo pathway MOB4A interacts with core kinases such as MST1/2 and LATS1/2 influencing downstream effectors like YAP/TAZ. The coordination between MOB4A and these kinases serves to restrain unwarranted cell proliferation maintaining tissue homeostasis. In the mTOR pathway MOB4A may liaise with proteins that influence cellular metabolism and growth responses.
- Associated diseases and disorders
MOB4A has a relationship with cancer and neurodegenerative diseases. In cancer aberrations in its interacting networks particularly within the Hippo pathway result in excessive cell proliferation due to atrophy of growth regulation. Aberrant MOB4A function connects with proteins like LATS1 contributing to tumorigenesis. In neurodegenerative disorders the protein's involvement in the mTOR signaling pathway suggests that disruptions could impact neuronal survival and contribute to conditions like Alzheimer's disease. The proper function of MOB4A is critical in maintaining neurological health.
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1 product image
SDS-PAGE - Recombinant Human MOB4A protein (ab140717)
15% SDS-PAGE analysis of ab140717 (3μg).
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