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AB198470

Recombinant human Monoacylglycerol Lipase/MGL protein

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Recombinant human Monoacylglycerol Lipase/MGL protein is a Human Full Length protein, in the 2 to 313 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Monoglyceride lipase, MGL, HU-K5, Lysophospholipase homolog, Lysophospholipase-like, Monoacylglycerol lipase, MAGL, MGLL

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Functional Studies - Recombinant human Monoacylglycerol Lipase/MGL protein (AB198470)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Monoacylglycerol Lipase/MGL protein (AB198470)

Specific activity of ab198470

SDS-PAGE - Recombinant human Monoacylglycerol Lipase/MGL protein (AB198470)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Monoacylglycerol Lipase/MGL protein (AB198470)

4-20% SDS PAGE anaylsis of 1.2 μg ab198470 stained with Coomassie

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus His tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Reaction was performed in 50 μl reaction mix containing 50 mM Tris pH 8.0, 150 mM NaCl, 0.5 mM 4-Methylumbelliferyl butyrate, and various amounts of ab198470. Reaction was incubated at 37°C for 15 minutes and fluorescent signal was measured at ex 360 nm, em 445 nm.

Accession

Q99685

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Preservative: 1.55% Imidazole Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab198470 is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

This product was previously labelled as Monoacylglycerol Lipase

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Sequence info

[{"sequence":"MHHHHHHGGGSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFKDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDPENLYFQGGGEFETGPEDPSSMPEESSPRRTPQSIPYQDLPHLVNADGQYLFCRYWKPTGTPKALIFVSHGAGEHSGRYEELARMLMGLDLLVFAHDHVGHGQSEGERMVVSDFHVFVRDVLQHVDSMQKDYPGLPVFLLGHSMGGAIAILTAAERPGHFAGMVLISPLVLANPESATTFKVLAAKVLNLVLPNLSLGPIDSSVLSRNKTEVDIYNSDPLICRAGLKVCFGIQLLNAVSRVERALPKLTVPFLLLQGSADRLCDSKGAYLLMELAKSQDKTLKIYEGAYHVLHKELPEVTNSVFHEINMWVSQRTATAGTASPP","proteinLength":"Full Length","predictedMolecularWeight":"62 kDa","actualMolecularWeight":null,"aminoAcidEnd":313,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q99685","tags":[{"tag":"GST","terminus":"N-Terminus"},{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Monoacylglycerol lipase (MGL) also known as MAGL or lipase 63 is an enzyme with a mass of approximately 33 kDa. MGL primarily functions in lipid metabolism by hydrolyzing 2-monoacylglycerol (2-MAG) into free fatty acids and glycerol. This enzyme is expressed in many tissues with high expression in the brain liver and adipose tissue. Its activity plays a significant role in the breakdown of monoacylglycerol an important intermediate in the endocannabinoid signaling system.
Biological function summary

MGL contributes to the regulation of energy homeostasis by controlling the levels of 2-monoacylglycerol. It catalyzes the final step in the degradation of the endocannabinoid 2-arachidonoylglycerol (2-AG) which is important for modulating inflammation pain and mood. MGL is not known to be part of a larger protein complex. In the brain its action helps terminate endocannabinoid signaling therefore influencing neurotransmission and synaptic plasticity.

Pathways

MGL plays a critical role in the endocannabinoid and glycerolipid metabolism pathways. In the endocannabinoid system it interacts with the cannabinoid receptors CB1 and CB2 by regulating 2-AG levels. The protein's role in glycerolipid metabolism involves its interaction with long-chain acyl-CoA synthetase and fatty acid oxidation pathways which are essential for maintaining lipid balance and cellular energy.

MGL has been linked to neurodegenerative diseases and cancer. In neurodegenerative diseases like Alzheimer’s dysregulation of MGL activity may lead to altered endocannabinoid signaling impacting neuronal health. In cancer increased MGL activity can promote tumor progression by providing cancer cells with fatty acids for growth and survival. MGL's interaction with proteins such as fatty acid synthase highlights its importance in cancer metabolism.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Converts monoacylglycerides to free fatty acids and glycerol (PubMed : 19029917, PubMed : 20079333, PubMed : 21049984, PubMed : 22969151, PubMed : 24368842). Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (PubMed : 19029917, PubMed : 20079333, PubMed : 21049984, PubMed : 22969151, PubMed : 24368842). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth (PubMed : 20079333).

Sequence similarities

Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family.

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Product protocols

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Target data

Converts monoacylglycerides to free fatty acids and glycerol (PubMed : 19029917, PubMed : 20079333, PubMed : 21049984, PubMed : 22969151, PubMed : 24368842). Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (PubMed : 19029917, PubMed : 20079333, PubMed : 21049984, PubMed : 22969151, PubMed : 24368842). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth (PubMed : 20079333).
See full target information MGLL
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Complementary Products

Primary Antibodies

AB24701

Anti-Monoacylglycerol Lipase/MGL antibody

Western blot - Anti-Monoacylglycerol Lipase/MGL antibody (AB24701)

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Secondary Antibodies

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Assay Kits

AB273326

Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric)

Functional Studies - Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) (AB273326)

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Biochemicals

AB141592

JZL-184, monoacylglycerol lipase (MAGL) inhibitor

Chemical Structure - JZL-184, monoacylglycerol lipase (MAGL) inhibitor (AB141592)

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