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AB198074

Recombinant Human Monoamine Oxidase A/MAO-A protein

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Recombinant Human Monoamine Oxidase A/MAO-A protein is a Human Fragment protein, in the 2 to 497 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE.

View Alternative Names

Amine oxidase [flavin-containing] A, Monoamine oxidase type A, MAO-A, MAOA

1 Images
SDS-PAGE - Recombinant Human Monoamine Oxidase A/MAO-A protein (AB198074)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Monoamine Oxidase A/MAO-A protein (AB198074)

4-20% SDS-PAGE analysis of ab198074 (1 μg) with Coomassie staining.

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

DDDDK tag N-Terminus His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P21397

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Previously labelled as Monoamine Oxidase A.

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Sequence info

[{"sequence":"MHHHHHHDYKDDDDKENQEKASIAGHMFDVVVIGGGISGLSAAKLLTEYGVSVLVLEARDRVGGRTYTIRNEHVDYVDVGGAYVGPTQNRILRLSKELGIETYKVNVSERLVQYVKGKTYPFRGAFPPVWNPIAYLDYNNLWRTIDNMGKEIPTDAPWEAQHADKWDKMTMKELIDKICWTKTARRFAYLFVNINVTSEPHEVSALWFLWYVKQCGGTTRIFSVTNGGQERKFVGGSGQVSERIMDLLGDQVKLNHPVTHVDQSSDNIIIETLNHEHYECKYVINAIPPTLTAKIHFRPELPAERNQLIQRLPMGAVIKCMMYYKEAFWKKKDYCGCMIIEDEDAPISITLDDTKPDGSLPAIMGFILARKADRLAKLHKEIRKKKICELYAKVLGSQEALHPVHYEEKNWCEEQYSGGCYTAYFPPGIMTQYGRVIRQPVGRIFFAGTETATKWSGYMEGAVEAGERAAREVLNGLGKVTEKDIWVQEPESKDVPAVEITHTFWERNLPS","proteinLength":"Fragment","predictedMolecularWeight":"58.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":497,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P21397","tags":[{"tag":"DDDDK","terminus":"N-Terminus"},{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Monoamine Oxidase A (MAO-A) also known as MAO protein is an enzyme that degrades neurotransmitters through oxidative deamination. It possesses a mass of approximately 59 kDa. This enzyme is found localized in the outer mitochondrial membrane of cells specifically in high concentrations in the brain liver and intestine. MAO-A plays an important role in the breakdown of monoamine neurotransmitters such as serotonin norepinephrine and dopamine which are critical for normal brain function.
Biological function summary

MAO-A acts to maintain proper neurotransmitter levels by catalyzing the oxidative deamination of monoamines. It is not part of a larger protein complex but functions independently to influence monoamine balance. Through its enzymatic activity MAO-A regulates several physiological processes including mood and emotional responses by controlling neurotransmitter availability and preventing their accumulation to neurotoxic levels.

Pathways

MAO-A operates within the catabolic pathways that reduce the bioavailability of monoamines. It pairs with proteins like Catechol-O-methyltransferase (COMT) in the metabolic degradation of catecholamines. This pathway is integral to managing the equilibrium of mood-related neurotransmitters and involves complex interactions with other enzymatic systems including MAO-B which targets different substrates.

MAO-A is closely associated with psychiatric conditions such as depression and anxiety. Altered MAO-A activity can dysregulate monoamine levels contributing to these disorders. Additionally research links MAO-A dysregulation to neurodegenerative diseases like Parkinson's disease where imbalances in monoamine levels play a role. This enzyme interacts with proteins involved in early stages of these diseases highlighting its potential as a target for therapeutic interventions using MAO inhibitors.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Catalyzes the oxidative deamination of primary and some secondary amine such as neurotransmitters, with concomitant reduction of oxygen to hydrogen peroxide and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues (PubMed : 18391214, PubMed : 20493079, PubMed : 24169519, PubMed : 8316221). Preferentially oxidizes serotonin (PubMed : 20493079, PubMed : 24169519). Also catalyzes the oxidative deamination of kynuramine to 3-(2-aminophenyl)-3-oxopropanal that can spontaneously condense to 4-hydroxyquinoline (By similarity).

Sequence similarities

Belongs to the flavin monoamine oxidase family.

Subcellular localisation

Mitochondrion outer membrane

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Product protocols

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Target data

Catalyzes the oxidative deamination of primary and some secondary amine such as neurotransmitters, with concomitant reduction of oxygen to hydrogen peroxide and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues (PubMed : 18391214, PubMed : 20493079, PubMed : 24169519, PubMed : 8316221). Preferentially oxidizes serotonin (PubMed : 20493079, PubMed : 24169519). Also catalyzes the oxidative deamination of kynuramine to 3-(2-aminophenyl)-3-oxopropanal that can spontaneously condense to 4-hydroxyquinoline (By similarity).
See full target information MAOA
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Primary Antibodies

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Primary Antibodies

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