Recombinant human/mouse FGF8 protein (Active) is a Human Full Length protein, in the 23 to 215 aa range, expressed in Escherichia coli, with >95% purity, < 0.05 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 0.05 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M Q V T V Q S S P N F T Q H V R E Q S L V T D Q L S R R L I R T Y Q L Y S R T S G K H V Q V L A N K R I N A M A E D G D P F A K L I V E T D T F G S R V R V R G A E T G L Y I C M N K K G K L I A K S N G K G K D C V F T E I V L E N N Y T A L Q N A K Y E G W Y M A F T R K G R P R K G S K T R Q H Q R E V H F M K R L P R G H H T T E Q S L R F E F L N Y P P F T R S L R G S Q R T W A P E P R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system (PubMed:16384934, PubMed:16597617, PubMed:8663044). Plays a role in neurite outgrowth in hippocampal cells (PubMed:21576111).
Alternative names
AIGF, FGF8, Fibroblast growth factor 8, FGF-8, Androgen-induced growth factor, Heparin-binding growth factor 8, HBGF-8
Recommended products
Recombinant human/mouse FGF8 protein (Active) is a Human Full Length protein, in the 23 to 215 aa range, expressed in Escherichia coli, with >95% purity, < 0.05 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 0.05 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The activity is determined by its ability to induce proliferation of mouse 3T3 cells and is typically less than 20 ng/mL. This corresponds to an expected specific activity of 5 x 104 units/mg.
- Accession
- P55075-1
- Animal free
- No
- Carrier free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute at 0.1 mg/mL in water
- Concentration
- Storage buffer
Constituents: 0.29% Sodium chloride, 0.08% Sodium phosphate
Sequence info
Amino acid sequence
- M Q V T V Q S S P N F T Q H V R E Q S L V T D Q L S R R L I R T Y Q L Y S R T S G K H V Q V L A N K R I N A M A E D G D P F A K L I V E T D T F G S R V R V R G A E T G L Y I C M N K K G K L I A K S N G K G K D C V F T E I V L E N N Y T A L Q N A K Y E G W Y M A F T R K G R P R K G S K T R Q H Q R E V H F M K R L P R G H H T T E Q S L R F E F L N Y P P F T R S L R G S Q R T W A P E P R
- Accession
- P37237
- Protein length
- Full Length
- Predicted molecular weight
- 22.5 kDa
- Amino acids
- 23 to 215
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
The purity of ab218083 is typically 97% as determined by reducing and non-reducing SDS-PAGE.
General info
- Function
Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system (PubMed:16384934, PubMed:16597617, PubMed:8663044). Plays a role in neurite outgrowth in hippocampal cells (PubMed:21576111).
- Sequence similarities
Belongs to the heparin-binding growth factors family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
There is 100% homology between Human 1-194 aa and mouse Mouse 23-215 aa FGF-8.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The FGF8 protein also known as fibroblast growth factor 8 plays a mechanical role in cell signaling and development. The FGF8 gene encodes two main isoforms: FGF8a and FGF8b each with different biological activities. FGF8 has a molecular mass of approximately 22-24 kDa. It expresses in developing tissues particularly in the embryo during early stages of vertebrate development. FGF8 interacts with fibroblast growth factor receptors (FGFRs) to initiate a cascade of signaling events leading to cellular responses.
- Biological function summary
The FGF8 protein influences the regulation of cell division growth and differentiation particularly in embryonic development. FGF8 an integral part of several signaling complexes activates pathways that guide mesoderm and neural plate induction. Its expression is essential for limb and brain development providing necessary signaling cues required for proper patterning and morphogenetic processes.
- Pathways
The FGF8 protein plays a central role in the MAPK/ERK signaling and PI3K/AKT pathways important mechanisms that govern cellular proliferation and survival. These pathways involve several proteins such as FGFR1 and FGFR2 which interact with FGF8 to mediate its effects. Through these pathways FGF8 coordinates with other members of the fibroblast growth factor family to regulate cellular functions in a concerted manner.
- Associated diseases and disorders
The FGF8 protein associates with various congenital malformations specifically conditions like Kallmann syndrome and certain craniofacial dysplasias. Mutations or dysregulation in FGF8 can disrupt normal signaling processes leading to abnormalities in development. The protein closely interacts with other growth factors and receptors such as FGFR1 which when altered might contribute to the pathogenesis of these disorders by affecting the signaling pathways they share with FGF8.
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3 product images
SDS-PAGE - Recombinant human/mouse FGF8 protein (Active) (ab218083)
SDS PAGE analysis of ab218083 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.
Functional Studies - Recombinant human/mouse FGF8 protein (Active) (ab218083)
Bioassay showing FGF8 induced proliferation of 3T3 cells using ab218083.
Functional Studies - Recombinant human/mouse FGF8 protein (Active) (ab218083)
Functional analysis of ab218083
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