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AB151687

Recombinant human/mouse/rat Activin A protein

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(1 Publication)

Recombinant human/mouse/rat Activin A protein is a Human Fragment protein, in the 311 to 426 aa range, expressed in Escherichia coli, with >95%, <1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC, FuncS.

View Alternative Names

Inhibin beta A chain, Activin beta-A chain, Erythroid differentiation protein, EDF, INHBA

3 Images
Functional Studies - Recombinant human/mouse/rat Activin A protein (AB151687)
  • FuncS

Supplier Data

Functional Studies - Recombinant human/mouse/rat Activin A protein (AB151687)

Induction of cytotoxicity in MPC-11 cells using ab151687. Assays were carried out in duplicate.

Functional Studies - Recombinant human/mouse/rat Activin A protein (AB151687)
  • FuncS

Unknown

Functional Studies - Recombinant human/mouse/rat Activin A protein (AB151687)

Functional analysis of ab151687

SDS-PAGE - Recombinant human/mouse/rat Activin A protein (AB151687)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human/mouse/rat Activin A protein (AB151687)

SDS PAGE analysis of ab151687 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

HPLC, FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The activity is determined by the ability to induce cytoxicity of MPC-11 cells and is typically less than 2 ng/mL. Corresponding to a specific activity of 5 x 105 units/mg.

Accession

P08476

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in water

Storage buffer

Constituents: 0.02% Trifluoroacetic acid

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB116029

Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (AB116029)

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Proteins & Peptides

AB7403

Streptavidin (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Streptavidin (HRP) (AB7403)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Assay Kits

AB201283

Rat PAI1 ELISA Kit (SERPINE1)

Recombinant|SimpleStep

Sandwich ELISA - Rat PAI1 ELISA Kit (SERPINE1) (AB201283)

  • 0
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Proteins & Peptides

AB50036

Recombinant human TGF beta 1 protein (Active)

Bioactive

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product shares 100% homology with amino acids 309 - 424 of Rat and Mouse Activin A.
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Sequence info

[{"sequence":"MGLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS","proteinLength":"Fragment","predictedMolecularWeight":"26.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":426,"aminoAcidStart":311,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P08476","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Activin A also known by its gene name INHBA is a protein involved in various mechanical processes in the human body. This protein forms part of the transforming growth factor-beta (TGF-β) superfamily and has a molecular weight of approximately 25 kDa for its dimeric forms. Activin A is expressed in many tissues including the pituitary gland reproductive organs and placenta. Researchers often use techniques like the Activin A ELISA test kit to measure its concentration in biological samples.
Biological function summary

Activin A plays a significant role in regulating cell differentiation proliferation and apoptosis. As part of a complex Activin A interacts with specific receptors on cell surfaces initiating intracellular signaling cascades that influence these vital processes. It acts as a growth factor in the reproductive system influencing follicle-stimulating hormone (FSH) secretion and is important in embryonic development and wound healing.

Pathways

Activin A participates in pathways such as the TGF-β signaling pathway and the SMAD protein signaling pathway. Within these pathways it interacts with receptor proteins like type I and type II Activin receptors and downstream SMAD proteins. These interactions enable Activin A to regulate gene expression relevant to various cellular functions.

Altered Activin A levels have associations with conditions like cancer and fibrotic diseases. Its overexpression links to some cancer types including colorectal and prostate cancer where it influences tumor growth and metastasis. Moreover its interaction with proteins like myostatin may contribute to muscle wasting disorders. The dysregulation of these pathways highlights the significance of Activin A in pathophysiology.
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Specifications

Form

Lyophilized

Additional notes

Purity is typically >95% as determined by HPLC, Reducing and Non-reducing SDS-PAGE.

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General info

Function

Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition.. Activin A is a homodimer of INHBA that plays a role in several essential biological processes including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis (PubMed : 3194407, PubMed : 16440334). Signals through type I (such as ACVR1B or ACVR1C) and type II receptors (such as ACVR2A, ACVR2B or BMPR2) which, upon ligand binding, phosphorylate SMAD2 and SMAD3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and modulate gene expression (PubMed : 10652306, PubMed : 24018044). Can also activate alternative non-canonical intracellular signaling pathways including the p38 MAPK, extracellular signal-regulated kinases 1/2 (ERK1/2) and c-Jun N-terminal kinases (JNKs) to modulate cell migration and differentiation (PubMed : 16440334). Alternatively, promotes osteoblastic differentiation via ACVRL1-SMAD1/5/9 pathway (PubMed : 34948289). In addition, can engage the type I receptor ACVR1 to form an ACVR1-activin A-type II receptor non-signaling complex (NSC) that renders receptors unavailable for engagement with BMPs, hence resulting in an apparent inhibition of ACVR1-mediated BMP signaling (PubMed : 26333933).. Inhibin A is a dimer of alpha/INHA and beta-A/INHBA that functions as a feedback regulator in the hypothalamic-pituitary-gonadal (HPG) axis. Inhibits the secretion of FSH from the anterior pituitary gland by acting on pituitary gonadotrope cells. Antagonizes activin A by binding to the proteoglycan, betaglycan, and forming a stable complex with and, thereby, sequestering type II activin receptors while excluding type I receptor.

Sequence similarities

Belongs to the TGF-beta family.

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Product protocols

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Target data

Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition.. Activin A is a homodimer of INHBA that plays a role in several essential biological processes including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis (PubMed : 3194407, PubMed : 16440334). Signals through type I (such as ACVR1B or ACVR1C) and type II receptors (such as ACVR2A, ACVR2B or BMPR2) which, upon ligand binding, phosphorylate SMAD2 and SMAD3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and modulate gene expression (PubMed : 10652306, PubMed : 24018044). Can also activate alternative non-canonical intracellular signaling pathways including the p38 MAPK, extracellular signal-regulated kinases 1/2 (ERK1/2) and c-Jun N-terminal kinases (JNKs) to modulate cell migration and differentiation (PubMed : 16440334). Alternatively, promotes osteoblastic differentiation via ACVRL1-SMAD1/5/9 pathway (PubMed : 34948289). In addition, can engage the type I receptor ACVR1 to form an ACVR1-activin A-type II receptor non-signaling complex (NSC) that renders receptors unavailable for engagement with BMPs, hence resulting in an apparent inhibition of ACVR1-mediated BMP signaling (PubMed : 26333933).. Inhibin A is a dimer of alpha/INHA and beta-A/INHBA that functions as a feedback regulator in the hypothalamic-pituitary-gonadal (HPG) axis. Inhibits the secretion of FSH from the anterior pituitary gland by acting on pituitary gonadotrope cells. Antagonizes activin A by binding to the proteoglycan, betaglycan, and forming a stable complex with and, thereby, sequestering type II activin receptors while excluding type I receptor.
See full target information Inhibin beta A chain
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Artificial organs 47:1298-1308 PubMed37032529

2023

Transplantation of insulin-producing cells derived from human MSCs to treat diabetes in a non-human primate model.

Applications

Unspecified application

Species

Unspecified reactive species

Zhu Zhoujun,Feng Bingzheng,Yang Yuwei,Zhang Yingying,Xu Zhiran,He Chunhua,Leng Jing,Tang Haibo,Li Wanli,Zhang Ting,Li Fujun,Chen Jibing,Gao Hongjun
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