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AB109955

Recombinant Human MPG/AAG protein (His tag C-Terminus)

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Recombinant Human MPG/AAG protein (His tag C-Terminus) is a Human Full Length protein, in the 1 to 298 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec, WB.

View Alternative Names

AAG, ANPG, MID1, MPG, DNA-3-methyladenine glycosylase, 3-alkyladenine DNA glycosylase, 3-methyladenine DNA glycosidase, ADPG, N-methylpurine-DNA glycosylase

2 Images
Western blot - Recombinant Human MPG/AAG protein (His tag C-Terminus) (AB109955)
  • WB

Unknown

Western blot - Recombinant Human MPG/AAG protein (His tag C-Terminus) (AB109955)

All lanes:

Anti-MPG antibody (<a href='/en-us/products/unavailable/mpg-antibody-ab55461'>ab55461</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human MPG/AAG protein (His tag C-Terminus) (ab109955) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

true

Exposure time: 10s

SDS-PAGE - Recombinant Human MPG/AAG protein (His tag C-Terminus) (AB109955)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human MPG/AAG protein (His tag C-Terminus) (AB109955)

SDS-PAGE analysis of Recombinant Human MPG/AAG protein (ab109955), under reducing conditions. Proteins visualized by coomassie blue stain.

Lane 1 : Molecular Weight Standards

Lane 2 : 3 μg Recombinant Human MPG/AAG protein

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

SDS-PAGE, WB, Mass Spec

applications

Biologically active

No

Accession

P29372

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 1% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.32% Tris HCl, 0.03% EDTA

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab109955 can be used as a WB positive control in conjunction with <a href='/en-us/products/unavailable/mpg-antibody-ab55461'>ab55461</a>.</p>" } } }
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Product details

This product was previously labelled as MPG
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Sequence info

[{"sequence":"MVTPALQMKKPKQFCRRMGQKKQRPARAGQPHSSSDAAQAPAEQPHSSSDAAQAPCPRERCLGPPTTPGPYRSIYFSSPKGHLTRLGLEFFDQPAVPLARAFLGQVLVRRLPNGTELRGRIVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYIIYGMYFCMNISSQGDGACVLLRALEPLEGLETMRQLRSTLRKGTASRVLKDRELCSGPSKLCQALAINKSFDQRDLAQDEAVWLERGPLEPSEPAVVAAARVGVGHAGEWARKPLRFYVRGSPWVSVVDRVAEQDTQALEHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"33.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":298,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P29372","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MPG also known as AAG or 3-methyladenine DNA glycosylase is a DNA repair protein with a molecular mass of approximately 33 kDa. It predominately acts to recognize and excise damaged bases from DNA playing a vital role in base excision repair (BER) mechanism. MPG/AAG is found in several tissues with high expression observed in liver and brain suggesting its importance in maintaining genomic stability in these areas.
Biological function summary

The MPG/AAG protein serves an essential function in cellular defense against genotoxic stress. It initiates the repair process by removing alkylated adenine and other modified bases from DNA. Although not directly identified as a part of any large protein complex MPG/AAG collaborates with other enzymatic components of BER such as DNA polymerase and ligase enzymes to facilitate complete and accurate DNA repair.

Pathways

MPG/AAG engages largely in the base excision repair pathway to correct single-base damage not addressed by nucleotide excision repair. Additionally its function ties into the cellular response to DNA alkylation a critical pathway concerning the maintenance of genomic integrity. MPG/AAG is often associated with DNA polymerase beta as a partner in extending and sealing the repaired strand ensuring the completion of the BER pathway.

The activity of MPG/AAG relates closely to cancer and neurodegenerative diseases. Alterations in MPG/AAG function have been linked to increased susceptibility to alkylation-induced tumorigenesis. Additionally impaired function can contribute to neuronal damage associating it with proteins involved in neurodegenerative processes such as Tau. These connections highlight the importance of MPG/AAG in both the initiation and progression of specific human diseases.
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Specifications

Form

Liquid

Additional notes

ab109955 was purified using conventional chromatography techniques.

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General info

Function

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.

Sequence similarities

Belongs to the DNA glycosylase MPG family.

Subcellular localisation

Mitochondrion matrix

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Product protocols

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Target data

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
See full target information MPG
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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (AB155092)

  • 5
  • 7

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