Recombinant Human MPG/AAG protein is a Human Full Length protein, in the 1 to 298 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS, WB.
Images
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, MS, WB
- Biologically active
No
Amino acid sequence
M V T P A L Q M K K P K Q F C R R M G Q K K Q R P A R A G Q P H S S S D A A Q A P A E Q P H S S S D A A Q A P C P R E R C L G P P T T P G P Y R S I Y F S S P K G H L T R L G L E F F D Q P A V P L A R A F L G Q V L V R R L P N G T E L R G R I V E T E A Y L G P E D E A A H S R G G R Q T P R N R G M F M K P G T L Y V Y I I Y G M Y F C M N I S S Q G D G A C V L L R A L E P L E G L E T M R Q L R S T L R K G T A S R V L K D R E L C S G P S K L C Q A L A I N K S F D Q R D L A Q D E A V W L E R G P L E P S E P A V V A A A R V G V G H A G E W A R K P L R F Y V R G S P W V S V V D R V A E Q D T Q A L E H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab109955 can be used as a WB positive control in conjunction with ab55461. |
Target data
Function
Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
Alternative names
DNA-3-methyladenine glycosylase, 3-alkyladenine DNA glycosylase, 3-methyladenine DNA glycosidase, ADPG, N-methylpurine-DNA glycosylase, MPG, MID1, AAG, ANPG
Recommended products
Recombinant Human MPG/AAG protein is a Human Full Length protein, in the 1 to 298 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS, WB.
Alternative names
DNA-3-methyladenine glycosylase, 3-alkyladenine DNA glycosylase, 3-methyladenine DNA glycosidase, ADPG, N-methylpurine-DNA glycosylase, MPG, MID1, AAG, ANPG
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, MS, WB
- Biologically active
No
- Accession
- P29372-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 30% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 1% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.32% Tris HCl, 0.03% EDTA
Sequence info
Amino acid sequence
M V T P A L Q M K K P K Q F C R R M G Q K K Q R P A R A G Q P H S S S D A A Q A P A E Q P H S S S D A A Q A P C P R E R C L G P P T T P G P Y R S I Y F S S P K G H L T R L G L E F F D Q P A V P L A R A F L G Q V L V R R L P N G T E L R G R I V E T E A Y L G P E D E A A H S R G G R Q T P R N R G M F M K P G T L Y V Y I I Y G M Y F C M N I S S Q G D G A C V L L R A L E P L E G L E T M R Q L R S T L R K G T A S R V L K D R E L C S G P S K L C Q A L A I N K S F D Q R D L A Q D E A V W L E R G P L E P S E P A V V A A A R V G V G H A G E W A R K P L R F Y V R G S P W V S V V D R V A E Q D T Q A L E H H H H H H
- Accession
- Protein length
Full Length
- Predicted molecular weight
33.9 kDa
- Amino acids
1 to 298
- Nature
Recombinant
- Tags
His tag C-Terminus
Specifications
- Form
Liquid
- Additional notes
ab109955 was purified using conventional chromatography techniques.
General info
- Function
Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
- Sequence similarities
Belongs to the DNA glycosylase MPG family.
- Subcellular localisation
Mitochondrion matrix, Mitochondrion nucleoid, Nucleus
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product was previously labelled as MPG
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MPG also known as AAG or 3-methyladenine DNA glycosylase is a DNA repair protein with a molecular mass of approximately 33 kDa. It predominately acts to recognize and excise damaged bases from DNA playing a vital role in base excision repair (BER) mechanism. MPG/AAG is found in several tissues with high expression observed in liver and brain suggesting its importance in maintaining genomic stability in these areas.
- Biological function summary
The MPG/AAG protein serves an essential function in cellular defense against genotoxic stress. It initiates the repair process by removing alkylated adenine and other modified bases from DNA. Although not directly identified as a part of any large protein complex MPG/AAG collaborates with other enzymatic components of BER such as DNA polymerase and ligase enzymes to facilitate complete and accurate DNA repair.
- Pathways
MPG/AAG engages largely in the base excision repair pathway to correct single-base damage not addressed by nucleotide excision repair. Additionally its function ties into the cellular response to DNA alkylation a critical pathway concerning the maintenance of genomic integrity. MPG/AAG is often associated with DNA polymerase beta as a partner in extending and sealing the repaired strand ensuring the completion of the BER pathway.
- Associated diseases and disorders
The activity of MPG/AAG relates closely to cancer and neurodegenerative diseases. Alterations in MPG/AAG function have been linked to increased susceptibility to alkylation-induced tumorigenesis. Additionally impaired function can contribute to neuronal damage associating it with proteins involved in neurodegenerative processes such as Tau. These connections highlight the importance of MPG/AAG in both the initiation and progression of specific human diseases.
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2 product images
SDS-PAGE - Recombinant Human MPG/AAG protein (ab109955)
SDS-PAGE analysis of Recombinant Human MPG/AAG protein (ab109955), under reducing conditions. Proteins visualized by coomassie blue stain.
Lane 1: Molecular Weight Standards
Lane 2: 3 μg Recombinant Human MPG/AAG protein
Western blot - Recombinant Human MPG/AAG protein (ab109955)
All lanes: Anti-MPG antibody (ab55461) at 1 µg/mL
All lanes: Western blot - Recombinant Human MPG/AAG protein (ab109955) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10s
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