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AB167917

Recombinant Human MPZL protein (His tag N-Terminus)

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Recombinant Human MPZL protein (His tag N-Terminus) is a Human Fragment protein, in the 38 to 162 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

PZR, UNQ849/PRO1787, MPZL1, Myelin protein zero-like protein 1, Protein zero-related

1 Images
SDS-PAGE - Recombinant Human MPZL protein (His tag N-Terminus) (AB167917)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MPZL protein (His tag N-Terminus) (AB167917)

15% SDS-PAGE analysis of ab167917 (3μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

O95297

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSLEVYTPKEIFVANGTQGKLTCKFKSTSTTGGLTSVSWSFQPEGADTTVSFFHYSQGQVYLGNYPPFKDRISWAGDLDKKDASINIENMQFIHNGTYICDVKNPPDIVVQPGHIRLYVVEKENLPV","proteinLength":"Fragment","predictedMolecularWeight":"16.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":162,"aminoAcidStart":38,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O95297","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein often referred to as MPZL or Myelin Protein Zero-like 1 (MPZL1) plays a significant role in cellular processes. It is a transmembrane glycoprotein with an approximated molecular mass of about 32.7 kDa. MPZL1 is expressed in various tissues with higher levels found in lung liver kidney and heart. Researchers also identify it under other names including Epithelial V-like Antigen (EVA) or IGSF8 and it typically contributes to cell adhesion and communication.
Biological function summary

MPZL1 supports critical functions like cell proliferation and differentiation through its interactions with other cellular components. It often associates with cytoplasmic signaling proteins suggesting its role in forming larger signaling complexes. MPZL1 engages in downstream signaling pathways when it binds to ligands influencing cellular dynamics and enabling proper tissue development and maintenance. Its interaction with other proteins furthers understanding of its involvement in cellular structural integrity.

Pathways

MPZL1 integrates into important cellular signaling cascades including the MAPK and PI3K-AKT pathways. Within these pathways MPZL1 modulates activity by contacting proteins like GRB2 and PIK3R1 influencing signals that are important for cellular growth and survival. Its activity in these pathways suggests an adaptive role in regulating cellular responses to external stimuli contributing to proper physiological function.

MPZL1 shows significant connection to cancer and fibrosis contexts. Researchers observe alterations in MPZL1 expression levels in certain cancers suggesting potential roles in tumor progression and metastasis. Additionally its link to fibrosis indicates a possible involvement in pathological tissue remodeling. Connections between MPZL1 PDGF receptors and fibrotic processes highlight the importance of understanding MPZL1 in disease mechanisms for potential therapeutic targets.
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Specifications

Form

Liquid

Additional notes

ab167917 is purified using conventional chromatography techniques.

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General info

Function

Cell surface receptor, which is involved in signal transduction processes. Recruits PTPN11/SHP-2 to the cell membrane and is a putative substrate of PTPN11/SHP-2. Is a major receptor for concanavalin-A (ConA) and is involved in cellular signaling induced by ConA, which probably includes Src family tyrosine-protein kinases. Isoform 3 seems to have a dominant negative role; it blocks tyrosine phosphorylation of MPZL1 induced by ConA. Isoform 1, but not isoform 2 and isoform 3, may be involved in regulation of integrin-mediated cell motility.

Sequence similarities

Belongs to the myelin P0 protein family.

Post-translational modifications

Phosphorylated on tyrosine residues upon stimulation with pervanadate and concanavalin-A (ConA). Phosphorylation at Tyr-241 and Tyr-263 is required for interaction with PTPN11/SHP-2. Dephosphorylated by PTPN11/SHP-2 (in vitro).. N-glycosylated. N-glycosylation is required for concanavalin A binding (PubMed:30392906).

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Product protocols

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Target data

Cell surface receptor, which is involved in signal transduction processes. Recruits PTPN11/SHP-2 to the cell membrane and is a putative substrate of PTPN11/SHP-2. Is a major receptor for concanavalin-A (ConA) and is involved in cellular signaling induced by ConA, which probably includes Src family tyrosine-protein kinases. Isoform 3 seems to have a dominant negative role; it blocks tyrosine phosphorylation of MPZL1 induced by ConA. Isoform 1, but not isoform 2 and isoform 3, may be involved in regulation of integrin-mediated cell motility.
See full target information MPZL1
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Complementary Products

Primary Antibodies

AB248994

Anti-MPZL antibody [EPR9229] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Western blot - Anti-MPZL antibody [EPR9229] - BSA and Azide free (AB248994)

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Cell Lines & Lysates

AB258053

Human MPZL1 (MPZL) knockout HeLa cell lysate

Western blot - Human MPZL1 (MPZL) knockout HeLa cell lysate (AB258053)

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Cell Lines & Lysates

AB265332

Human MPZL1 (MPZL) knockout HeLa cell line

Advanced Validation

Western blot - Human MPZL1 (MPZL) knockout HeLa cell line (AB265332)

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Primary Antibodies

AB188075

Anti-TCFL5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCFL5 antibody (AB188075)

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