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AB137141

Recombinant Human MRRF protein (His tag N-Terminus)

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Recombinant Human MRRF protein (His tag N-Terminus) is a Human Full Length protein, in the 56 to 262 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

RRF, mtRRF, MRRF

1 Images
SDS-PAGE - Recombinant Human MRRF protein (His tag N-Terminus) (AB137141)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MRRF protein (His tag N-Terminus) (AB137141)

15% SDS-PAGE analysis of 3 µg ab137141.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q96E11

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAKDSLRKVRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG","proteinLength":"Full Length","predictedMolecularWeight":"25.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":262,"aminoAcidStart":56,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96E11","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRRF also known as Mitochondrial Ribosome Recycling Factor is a protein involved mechanically in the process of ribosome recycling after mRNA translation. It facilitates the disassembly of ribosomal subunits preparing them for a new cycle of protein synthesis. MRRF has a molecular mass of approximately 29 kDa. The protein is localized in the mitochondria which is where it performs its function in mitochondrial ribosome recycling.
Biological function summary

MRRF plays a significant role in mitochondrial translation by promoting the release of ribosomes from mRNA enabling further rounds of translation. It functions as part of the mitochondrial translation machinery and is involved in the maintenance of mitochondrial protein synthesis efficiency. The interaction with other mitochondrial translation factors allows MRRF to efficiently mediate the disassembly of post-termination ribosomal complexes.

Pathways

MRRF is critically involved in the mitochondrial translation process a subset of the broader protein synthesis pathway within the cell. It actively participates in the recycling phase of mitochondrial translation ensuring the effective reuse of ribosomal components. MRRF interacts closely with other proteins such as mtEFG1 (Mitochondrial Elongation Factor G1) to facilitate ribosome recycling and promote cellular energy production linking mitochondrial translation to oxidative phosphorylation.

Dysfunction or mutations of MRRF have associations with mitochondrial diseases which often result from impaired protein synthesis in mitochondria. Such conditions can include mitochondrial encephalomyopathy and other neuromuscular disorders. MRRF may also relate to proteins like MFN2 (Mitofusin 2) which are important in maintaining mitochondrial network stability and function; disruptions in these proteins can compound to exacerbate mitochondrial dysfunction-related disorders.

Specifications

Form

Liquid

Additional notes

ab137141 is purified using conventional chromatography techniques.

General info

Function

Responsible for the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis (PubMed : 19716793, PubMed : 33878294). Acts in collaboration with GFM2 (PubMed : 33878294). Promotes mitochondrial ribosome recycling by dissolution of intersubunit contacts (PubMed : 33878294).

Sequence similarities

Belongs to the RRF family.

Subcellular localisation

Mitochondrion

Product protocols

Target data

Responsible for the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis (PubMed : 19716793, PubMed : 33878294). Acts in collaboration with GFM2 (PubMed : 33878294). Promotes mitochondrial ribosome recycling by dissolution of intersubunit contacts (PubMed : 33878294).
See full target information Ribosome-recycling factor, mitochondrial

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