Recombinant Human MSH2 protein is a Human Full Length protein, in the 1 to 934 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M A V Q P K E T L Q L E S A A E V G F V R F F Q G M P E K P T T T V R L F D R G D F Y T A H G E D A L L A A R E V F K T Q G V I K Y M G P A G A K N L Q S V V L S K M N F E S F V K D L L L V R Q Y R V E V Y K N R A G N K A S K E N D W Y L A Y K A S P G N L S Q F E D I L F G N N D M S A S I G V V G V K M S A V D G Q R Q V G V G Y V D S I Q R K L G L C E F P D N D Q F S N L E A L L I Q I G P K E C V L P G G E T A G D M G K L R Q I I Q R G G I L I T E R K K A D F S T K D I Y Q D L N R L L K G K K G E Q M N S A V L P E M E N Q V A V S S L S A V I K F L E L L S D D S N F G Q F E L T T F D F S Q Y M K L D I A A V R A L N L F Q G S V E D T T G S Q S L A A L L N K C K T P Q G Q R L V N Q W I K Q P L M D K N R I E E R L N L V E A F V E D A E L R Q T L Q E D L L R R F P D L N R L A K K F Q R Q A A N L Q D C Y R L Y Q G I N Q L P N V I Q A L E K H E G K H Q K L L L A V F V T P L T D L R S D F S K F Q E M I E T T L D M D Q V E N H E F L V K P S F D P N L S E L R E I M N D L E K K M Q S T L I S A A R D L G L D P G K Q I K L D S S A Q F G Y Y F R V T C K E E K V L R N N K N F S T V D I Q K N G V K F T N S K L T S L N E E Y T K N K T E Y E E A Q D A I V K E I V N I S S G Y V E P M Q T L N D V L A Q L D A V V S F A H V S N G A P V P Y V R P A I L E K G Q G R I I L K A S R H A C V E V Q D E I A F I P N D V Y F E K D K Q M F H I I T G P N M G G K S T Y I R Q T G V I V L M A Q I G C F V P C E S A E V S I V D C I L A R V G A G D S Q L K G V S T F M A E M L E T A S I L R S A T K D S L I I I D E L G R G T S T Y D G F G L A W A I S E Y I A T K I G A F C M F A T H F H E L T A L A N Q I P T V N N L H V T A L T T E E T L T M L Y Q V K K G V C D Q S F G I H V A E L A N F P K H V I E C A K Q K A L E L E E F Q Y I G E S Q G Y D I M E P A A K K C Y L E R E Q G E K I I Q E F L S K V K Q M P F T E M S E E N I T I K L K Q L K A E V I A K N N S F V N E I I S R I K V T T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containing DNA strand (PubMed:26300262). ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
Alternative names
DNA mismatch repair protein Msh2, hMSH2, MutS protein homolog 2, MSH2
Recommended products
Recombinant Human MSH2 protein is a Human Full Length protein, in the 1 to 934 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P43246-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Sequence info
Amino acid sequence
- M A V Q P K E T L Q L E S A A E V G F V R F F Q G M P E K P T T T V R L F D R G D F Y T A H G E D A L L A A R E V F K T Q G V I K Y M G P A G A K N L Q S V V L S K M N F E S F V K D L L L V R Q Y R V E V Y K N R A G N K A S K E N D W Y L A Y K A S P G N L S Q F E D I L F G N N D M S A S I G V V G V K M S A V D G Q R Q V G V G Y V D S I Q R K L G L C E F P D N D Q F S N L E A L L I Q I G P K E C V L P G G E T A G D M G K L R Q I I Q R G G I L I T E R K K A D F S T K D I Y Q D L N R L L K G K K G E Q M N S A V L P E M E N Q V A V S S L S A V I K F L E L L S D D S N F G Q F E L T T F D F S Q Y M K L D I A A V R A L N L F Q G S V E D T T G S Q S L A A L L N K C K T P Q G Q R L V N Q W I K Q P L M D K N R I E E R L N L V E A F V E D A E L R Q T L Q E D L L R R F P D L N R L A K K F Q R Q A A N L Q D C Y R L Y Q G I N Q L P N V I Q A L E K H E G K H Q K L L L A V F V T P L T D L R S D F S K F Q E M I E T T L D M D Q V E N H E F L V K P S F D P N L S E L R E I M N D L E K K M Q S T L I S A A R D L G L D P G K Q I K L D S S A Q F G Y Y F R V T C K E E K V L R N N K N F S T V D I Q K N G V K F T N S K L T S L N E E Y T K N K T E Y E E A Q D A I V K E I V N I S S G Y V E P M Q T L N D V L A Q L D A V V S F A H V S N G A P V P Y V R P A I L E K G Q G R I I L K A S R H A C V E V Q D E I A F I P N D V Y F E K D K Q M F H I I T G P N M G G K S T Y I R Q T G V I V L M A Q I G C F V P C E S A E V S I V D C I L A R V G A G D S Q L K G V S T F M A E M L E T A S I L R S A T K D S L I I I D E L G R G T S T Y D G F G L A W A I S E Y I A T K I G A F C M F A T H F H E L T A L A N Q I P T V N N L H V T A L T T E E T L T M L Y Q V K K G V C D Q S F G I H V A E L A N F P K H V I E C A K Q K A L E L E E F Q Y I G E S Q G Y D I M E P A A K K C Y L E R E Q G E K I I Q E F L S K V K Q M P F T E M S E E N I T I K L K Q L K A E V I A K N N S F V N E I I S R I K V T T
- Accession
- P43246
- Protein length
- Full Length
- Predicted molecular weight
- 128.81 kDa
- Amino acids
- 1 to 934
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containing DNA strand (PubMed:26300262). ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
- Sequence similarities
Belongs to the DNA mismatch repair MutS family.
- Post-translational modifications
Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MSH2 also known as MutS Homolog 2 is a human protein with a molecular weight of approximately 100 kDa. It is an important component of the DNA mismatch repair system and plays an essential role in maintaining genomic stability by recognizing and repairing mismatched nucleotides during DNA replication. Expression of the MSH2 protein occurs broadly in dividing cells across various tissues with notable presence in tissues with high proliferation rates such as the colon and the endometrium. Additionally detection and quantification of MSH2 are often performed using methodologies such as MSH2 ELISA which aids in the assessment of its expression levels in different biological samples.
- Biological function summary
Components are identified in mismatch repair where MSH2 forms a heterodimer with MSH6 known as the MutSα complex or with MSH3 known as the MutSβ complex. This heterodimerization is critical for the initial steps in the recognition and binding of mismatch errors on the DNA strand. MSH2 complex formation enables it to scan the DNA for errors facilitating the recruitment of additional repair proteins. The activity of MSH2 in these complexes is important in preserving the fidelity of genetic information and prevents mutations that could lead to genomic instability.
- Pathways
MSH2 operates within the DNA damage response and repair pathways. The protein is a core component of the mismatch repair pathway which corrects DNA replication errors that elude proofreading activity of DNA polymerases. It interacts with other proteins such as MLH1 and PMS2 forming a synergistic function that amplifies the capacity to recognize and initiate repair of mismatches. The pathway involving MSH2 not only repairs mismatched bases but also plays a role in cell cycle control checkpoints and apoptosis evidencing its pivotal role in maintaining cell cycle integrity.
- Associated diseases and disorders
Studies show that MSH2 is strongly associated with Lynch syndrome an autosomal dominant inherited condition that increases the risk of colorectal cancer. Mutations in the MSH2 gene impair its mismatch repair function and lead to microsatellite instability a hallmark of cancer cells in this disorder. Furthermore alterations in the MSH2 protein also relate to glioblastomas with correlations observed between MSH2 expression levels and tumor progression. These conditions exemplify the important role of MSH2 and its interaction with other DNA repair proteins in preventing cancerous developments.
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1 product image
SDS-PAGE - Recombinant Human MSH2 protein (ab114351)
ab114351 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.
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