Recombinant Human MSI2 protein is a Human Full Length protein, in the 1 to 328 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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- Oncogenesis 10:292021Musashi-2 (MSI2) regulates epidermal growth factor receptor (EGFR) expression and response to EGFR inhibitors in EGFR-mutated non-small cell lung cancer (NSCLC).Applications:Unspecified applicationReactive species:Unspecified reactive speciesPetr Makhov,Igor Bychkov,Bulat Faezov,Alexander Deneka,Alexander Kudinov,Emmanuelle Nicolas,Rohan Brebion,Eleanor Avril,Kathy Q Cai,Leonid V Kharin,Mark Voloshin,Elena Frantsiyants,Nikolay Karnaukhov,Oleg I Kit,Iuliia Topchu,Rushaniya Fazliyeva,Anna S Nikonova,Ilya G Serebriiskii,Hossein Borghaei,Martin Edelman,Essel Dulaimi,Erica A Golemis,Yanis BoumberPubMed 33723247
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M E A N G S Q G T S G S A N D S Q H D P G K M F I G G L S W Q T S P D S L R D Y F S K F G E I R E C M V M R D P T T K R S R G F G F V T F A D P A S V D K V L G Q P H H E L D S K T I D P K V A F P R R A Q P K M V T R T K K I F V G G L S A N T V V E D V K Q Y F E Q F G K V E D A M L M F D K T T N R H R G F G F V T F E N E D V V E K V C E I H F H E I N N K M V E C K K A Q P K E V M F P P G T R G R A R G L P Y T M D A F M L G M G M L G Y P N F V A T Y G R G Y P G F A P S Y G Y Q F P G F P A A A Y G P V A A A A V A A A R G S G S N P A R P G G F P G A N S P G P V A D L Y G P A S Q D S G V G N Y I S A A S P Q P G S G F G H G I A G P L I A T A F T N G Y H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).
Alternative names
RNA-binding protein Musashi homolog 2, Musashi-2, MSI2
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Recombinant Human MSI2 protein is a Human Full Length protein, in the 1 to 328 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q96DH6-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M E A N G S Q G T S G S A N D S Q H D P G K M F I G G L S W Q T S P D S L R D Y F S K F G E I R E C M V M R D P T T K R S R G F G F V T F A D P A S V D K V L G Q P H H E L D S K T I D P K V A F P R R A Q P K M V T R T K K I F V G G L S A N T V V E D V K Q Y F E Q F G K V E D A M L M F D K T T N R H R G F G F V T F E N E D V V E K V C E I H F H E I N N K M V E C K K A Q P K E V M F P P G T R G R A R G L P Y T M D A F M L G M G M L G Y P N F V A T Y G R G Y P G F A P S Y G Y Q F P G F P A A A Y G P V A A A A V A A A R G S G S N P A R P G G F P G A N S P G P V A D L Y G P A S Q D S G V G N Y I S A A S P Q P G S G F G H G I A G P L I A T A F T N G Y H
- Accession
- Q96DH6
- Protein length
- Full Length
- Predicted molecular weight
- 37.6 kDa
- Amino acids
- 1 to 328
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab167853 was purified using conventional chromatography techniques.
General info
- Function
RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).
- Sequence similarities
Belongs to the Musashi family.
- Post-translational modifications
Phosphorylated.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Musashi-2 (MSI2) is a member of the Musashi RNA‐binding protein family also labeled as MSI-2. The protein is approximately 38 kDa in size. It expresses primarily in stem cells and various tumor types including hematopoietic cells and neural tissue. MSI2 plays a role in maintaining stem cell self-renewal and differentiation by regulating mRNA translation through binding to specific target RNAs.
- Biological function summary
MSI2 contributes to the regulation of gene expression with a significant impact on cell fate decisions. It acts as part of a post-transcriptional regulatory complex that controls the stability and translation of specific mRNA targets. MSI2 influences pathways involved in stem cell maintenance and differentiation linking it to cellular processes like proliferation and apoptosis. Its activity is important for normal development and the maintenance of undifferentiated cell states.
- Pathways
MSI2 has important roles in the Notch and Wnt signaling pathways. These pathways are vital for stem cell maintenance and tissue regeneration. MSI2 regulates components of these pathways interacting with proteins such as beta-catenin in Wnt signaling. This regulation enhances its involvement in controlling cell proliferation and differentiation contributing to signaling processes that determine cellular behavior.
- Associated diseases and disorders
MSI2 is linked to acute myeloid leukemia (AML) and certain types of solid tumors. Overexpression of MSI2 often correlates with poor prognosis in AML where it impacts cancer progression by modulating genes associated with cell growth. Additionally its interaction with oncogenic proteins like BCR-ABL suggests its involvement in cancerogenesis through disease-linked pathways. Research indicates MSI2 as a potential target for therapeutic intervention in such malignancies.
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SDS-PAGE - Recombinant Human MSI2 protein (ab167853)
15% SDS-PAGE analysis of 3μg ab167853.
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