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AB60341

Recombinant human MST4 protein

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Recombinant human MST4 protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

MASK, MST4, STK26, Serine/threonine-protein kinase 26, MST3 and SOK1-related kinase, Mammalian STE20-like protein kinase 4, Serine/threonine-protein kinase MASK, MST-4, STE20-like kinase MST4

4 Images
Functional Studies - Recombinant human MST4 protein (AB60341)
  • FuncS

Unknown

Functional Studies - Recombinant human MST4 protein (AB60341)

The specific activity of MST4 (ab60341) was determined to be 23 nmol/min/mg as per activity assay protocol

Functional Studies - Recombinant human MST4 protein (AB60341)
  • FuncS

Unknown

Functional Studies - Recombinant human MST4 protein (AB60341)

Sample Kinase Activity Plot.

Western blot - Recombinant human MST4 protein (AB60341)
  • WB

Unknown

Western blot - Recombinant human MST4 protein (AB60341)

ab52491 recognizes the full length tagged recombinant MST4 protein (ab60341) which has an expected molecular weight of 72 kDa.

All lanes:

Western blot - Anti-MST4 antibody [EP1864Y] (<a href='/en-us/products/primary-antibodies/mst4-antibody-ep1864y-ab52491'>ab52491</a>) at 1/20000 dilution

All lanes:

Western blot - Recombinant human MST4 protein (ab60341) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 47 kDa

false

Exposure time: 20s

SDS-PAGE - Recombinant human MST4 protein (AB60341)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human MST4 protein (AB60341)

SDS PAGE analysis of ab60341

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

WB, SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Active

Accession

Q9P289

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab60341 can be used as a WB positive control in conjunction with <a href='/en-us/products/primary-antibodies/mst4-antibody-ep1864y-ab52491'>ab52491</a>.</p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Kinase assay: Use at an assay dependent dilution. Specific activity: 23 nmol/min/mg.</p>" } } }
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Product details

ab43614 (Human Myelin Basic Protein full length protein) can be utilized as a substrate for assessing Kinase activity
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9P289","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MST4 also known as mammalian sterile 20-like kinase 4 is a serine/threonine kinase with a molecular mass of approximately 53 kDa. It belongs to the germinal center kinase (GCK) family and is expressed in various tissues showing higher levels in the brain and testis. MST4 localizes to the Golgi apparatus where it plays a role in regulating cellular processes.
Biological function summary

MST4 contributes to cell growth apoptosis and stress response signaling. It does not operate alone; MST4 is a part of the STRIPAK complex. The protein involves itself in the regulation of the actin cytoskeleton thereby influencing cell motility and polarity. By modulating these processes MST4 can impact developmental and stress-induced cellular pathways.

Pathways

MST4 participates in the Hippo signaling pathway which regulates organ size by controlling cell proliferation and apoptosis. In this pathway MST4 interacts with other proteins such as MST1 and MST2 to execute its functions. MST4 also participates in the MAPK signaling pathway where it connects to various cellular stress responses contributing to the transcriptional activity of downstream effectors.

MST4 has implications in cancer and neurodegenerative diseases. Alterations in MST4 expression or activity have been associated with tumor progression in several cancers. Additionally it's linked to neurodegeneration through its interactions with proteins such as Tau where dysregulated pathways may lead to pathological conditions. These connections highlight the importance of MST4 in maintaining cellular homeostasis and the implications of its dysfunction in disease states.
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Specifications

Form

Liquid

Additional notes

Purity: >90% as determined by densitometry. Affinity purified.

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General info

Function

Serine/threonine-protein kinase that acts as a mediator of cell growth (PubMed : 11641781, PubMed : 17360971). Modulates apoptosis (PubMed : 11641781, PubMed : 17360971). In association with STK24 negatively regulates Golgi reorientation in polarized cell migration upon RHO activation (PubMed : 27807006). Phosphorylates ATG4B at 'Ser-383', thereby increasing autophagic flux (PubMed : 29232556). Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed : 18782753).

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

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Product protocols

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Target data

Serine/threonine-protein kinase that acts as a mediator of cell growth (PubMed : 11641781, PubMed : 17360971). Modulates apoptosis (PubMed : 11641781, PubMed : 17360971). In association with STK24 negatively regulates Golgi reorientation in polarized cell migration upon RHO activation (PubMed : 27807006). Phosphorylates ATG4B at 'Ser-383', thereby increasing autophagic flux (PubMed : 29232556). Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed : 18782753).
See full target information STK26
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Complementary Products

Primary Antibodies

AB52491

Anti-MST4 antibody [EP1864Y]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST4 antibody [EP1864Y] (AB52491)

  • 0
  • 0
Cell Lines & Lysates

AB94208

MST4 overexpression 293T lysate (whole cell)

Western blot - MST4 overexpression 293T lysate (whole cell) (AB94208)

  • 0
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Primary Antibodies

AB171978

Anti-TIMMDC1/C3orf1 antibody [EPR11807]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-TIMMDC1/C3orf1 antibody [EPR11807] (AB171978)

  • 0
  • 0
Primary Antibodies

AB308612

Anti-CP2c antibody [14/LSF] - BSA and Azide free

Recombinant

Immunoprecipitation - Anti-CP2c antibody [14/LSF] - BSA and Azide free (AB308612)

  • 0
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