Recombinant Human MSY2/YBOX2/YBX2 protein is a Human Full Length protein, in the 1 to 364 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M S E V E A A A V A T A V P A A T V P A T A A G V V A V V V P V P A G E P Q K G G G A G G G G G A A S G P A A G T P S A P G S R T P G N P A T A V S G T P A P P A R S Q A D K P V L A I Q V L G T V K W F N V R N G Y G F I N R N D T K E D V F V H Q T A I K R N N P R K F L R S V G D G E T V E F D V V E G E K G A E A T N V T G P G G V P V K G S R Y A P N R R K S R R F I P R P P S V A P P P M V A E I P S A G T G P G S K G E R A E D S G Q R P R R W C P P P F F Y R R R F V R G P R P P N Q Q Q P I E G T D R V E P K E T A P L E G H Q Q Q G D E R V P P P R F R P R Y R R P F R P R P R Q Q P T T E G G D G E T K P S Q G P A D G S R P E P Q R P R N R P Y F Q R R R Q Q A P G P Q Q A P G P R Q P A A P E T S A P V N S G D P T T T I L E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Major constituent of messenger ribonucleoprotein particles (mRNPs). Involved in the regulation of the stability and/or translation of germ cell mRNAs. Binds to Y-box consensus promoter element. Binds to full-length mRNA with high affinity in a sequence-independent manner. Binds to short RNA sequences containing the consensus site 5'-UCCAUCA-3' with low affinity and limited sequence specificity. Its binding with maternal mRNAs is necessary for its cytoplasmic retention. May mark specific mRNAs (those transcribed from Y-box promoters) in the nucleus for cytoplasmic storage, thereby linking transcription and mRNA storage/translational delay (By similarity).
Alternative names
CSDA3, MSY2, YBX2, Y-box-binding protein 2, Contrin, DNA-binding protein C, Germ cell-specific Y-box-binding protein, MSY2 homolog, Dbpc
Recommended products
Recombinant Human MSY2/YBOX2/YBX2 protein is a Human Full Length protein, in the 1 to 364 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q9Y2T7-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M S E V E A A A V A T A V P A A T V P A T A A G V V A V V V P V P A G E P Q K G G G A G G G G G A A S G P A A G T P S A P G S R T P G N P A T A V S G T P A P P A R S Q A D K P V L A I Q V L G T V K W F N V R N G Y G F I N R N D T K E D V F V H Q T A I K R N N P R K F L R S V G D G E T V E F D V V E G E K G A E A T N V T G P G G V P V K G S R Y A P N R R K S R R F I P R P P S V A P P P M V A E I P S A G T G P G S K G E R A E D S G Q R P R R W C P P P F F Y R R R F V R G P R P P N Q Q Q P I E G T D R V E P K E T A P L E G H Q Q Q G D E R V P P P R F R P R Y R R P F R P R P R Q Q P T T E G G D G E T K P S Q G P A D G S R P E P Q R P R N R P Y F Q R R R Q Q A P G P Q Q A P G P R Q P A A P E T S A P V N S G D P T T T I L E
- Accession
- Q9Y2T7
- Protein length
- Full Length
- Amino acids
- 1 to 364
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Major constituent of messenger ribonucleoprotein particles (mRNPs). Involved in the regulation of the stability and/or translation of germ cell mRNAs. Binds to Y-box consensus promoter element. Binds to full-length mRNA with high affinity in a sequence-independent manner. Binds to short RNA sequences containing the consensus site 5'-UCCAUCA-3' with low affinity and limited sequence specificity. Its binding with maternal mRNAs is necessary for its cytoplasmic retention. May mark specific mRNAs (those transcribed from Y-box promoters) in the nucleus for cytoplasmic storage, thereby linking transcription and mRNA storage/translational delay (By similarity).
- Post-translational modifications
Phosphorylated during oocyte maturation and dephosphorylated following egg activation. Phosphorylated in vitro by a kinase activity associated with testicular mRNPs. Dephosphorylation leads to a decrease in its affinity to bind RNA in vitro (By similarity).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as MSY2/YBOX2.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MSY2 also known as YBOX2 or YBX2 is a protein involved in gene regulation specifically affecting mRNA stability and translation. The protein has a molecular mass of approximately 36 kDa. MSY2 is a germ cell-specific protein primarily expressed in testes and ovaries. It plays an important role during gametogenesis and early embryogenesis by binding with mRNA and forming messenger ribonucleoprotein (mRNP) complexes and therefore controlling mRNA metabolism.
- Biological function summary
MSY2 functions as a regulator of mRNA translation and storage. It is part of a ribonucleoprotein complex that safeguards mRNAs during gametogenesis. This protein displays specificity in binding to Y-box motifs on mRNA which allows it to regulate gene expression and protein synthesis at a posttranscriptional level. Its function ensures correct development of germ cells contributing to reproductive success.
- Pathways
MSY2 integrates into posttranscriptional regulatory mechanisms and is important for mRNA stability within these pathways. One such pathway involves cytoplasmic deadenylation and translational control during oocyte maturation. MSY2’s role also extends to working closely with other proteins such as YBX1 which shares similar functions in mRNA regulation further highlighting the interconnected nature of its activity.
- Associated diseases and disorders
MSY2’s deregulation can lead to fertility issues particularly affecting spermatogenesis. Aberrant expression of MSY2 affects mRNA stability and may result in testicular dysfunction. It also connects with proteins like DAZL which is another germ cell-specific regulator suggesting that perturbations in this network can affect germ cell viability and lead to reproductive disorders. Proper expression and regulation of MSY2 are therefore essential for maintaining reproductive health.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human MSY2/YBOX2/YBX2 protein (ab162411)
ab162411 on a 12.5% SDS-PAGE stained with Coomassie Blue.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com