Recombinant Human MTHFD1 protein is a Human Full Length protein, in the 1 to 935 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M A P A E I L N G K E I S A Q I R A R L K N Q V T Q L K E Q V P G F T P R L A I L Q V G N R D D S N L Y I N V K L K A A E E I G I K A T H I K L P R T T T E S E V M K Y I T S L N E D S T V H G F L V Q L P L D S E N S I N T E E V I N A I A P E K D V D G L T S I N A G R L A R G D L N D C F I P C T P K G C L E L I K E T G V P I A G R H A V V V G R S K I V G A P M H D L L L W N N A T V T T C H S K T A H L D E E V N K G D I L V V A T G Q P E M V K G E W I K P G A I V I D C G I N Y V P D D K K P N G R K V V G D V A Y D E A K E R A S F I T P V P G G V G P M T V A M L M Q S T V E S A K R F L E K F K P G K W M I Q Y N N L N L K T P V P S D I D I S R S C K P K P I G K L A R E I G L L S E E V E L Y G E T K A K V L L S A L E R L K H R P D G K Y V V V T G I T P T P L G E G K S T T T I G L V Q A L G A H L Y Q N V F A C V R Q P S Q G P T F G I K G G A A G G G Y S Q V I P M E E F N L H L T G D I H A I T A A N N L V A A A I D A R I F H E L T Q T D K A L F N R L V P S V N G V R R F S D I Q I R R L K R L G I E K T D P T T L T D E E I N R F A R L D I D P E T I T W Q R V L D T N D R F L R K I T I G Q A P T E K G H T R T A Q F D I S V A S E I M A V L A L T T S L E D M R E R L G K M V V A S S K K G E P V S A E D L G V S G A L T V L M K D A I K P N L M Q T L E G T P V F V H A G P F A N I A H G N S S I I A D Q I A L K L V G P E G F V V T E A G F G A D I G M E K F F N I K C R Y S G L C P H V V V L V A T V R A L K M H G G G P T V T A G L P L P K A Y I Q E N L E L V E K G F S N L K K Q I E N A R M F G I P V V V A V N A F K T D T E S E L D L I S R L S R E H G A F D A V K C T H W A E G G K G A L A L A Q A V Q R A A Q A P S S F Q L L Y D L K L P V E D K I R I I A Q K I Y G A D D I E L L P E A Q H K A E V Y T K Q G F G N L P I C M A K T H L S L S H N P E Q K G V P T G F I L P I R D I R A S V G A G F L Y P L V G T M S T M P G L P T R P C F Y D I D L D P E T E Q V N G L F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes Recombinant protein. |
Target data
Function
Trifunctional enzyme that catalyzes the interconversion of three forms of one-carbon-substituted tetrahydrofolate: (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate, 5,10-methenyltetrahydrofolate and (6S)-10-formyltetrahydrofolate (PubMed:10828945, PubMed:18767138, PubMed:1881876). These derivatives of tetrahydrofolate are differentially required in nucleotide and amino acid biosynthesis, (6S)-10-formyltetrahydrofolate being required for purine biosynthesis while (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate is used for serine and methionine biosynthesis for instance (PubMed:18767138, PubMed:25633902).
Alternative names
MTHFC, MTHFD, MTHFD1, C1-THF synthase, Epididymis secretory sperm binding protein
Recommended products
Recombinant Human MTHFD1 protein is a Human Full Length protein, in the 1 to 935 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P11586-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Sequence info
Amino acid sequence
- M A P A E I L N G K E I S A Q I R A R L K N Q V T Q L K E Q V P G F T P R L A I L Q V G N R D D S N L Y I N V K L K A A E E I G I K A T H I K L P R T T T E S E V M K Y I T S L N E D S T V H G F L V Q L P L D S E N S I N T E E V I N A I A P E K D V D G L T S I N A G R L A R G D L N D C F I P C T P K G C L E L I K E T G V P I A G R H A V V V G R S K I V G A P M H D L L L W N N A T V T T C H S K T A H L D E E V N K G D I L V V A T G Q P E M V K G E W I K P G A I V I D C G I N Y V P D D K K P N G R K V V G D V A Y D E A K E R A S F I T P V P G G V G P M T V A M L M Q S T V E S A K R F L E K F K P G K W M I Q Y N N L N L K T P V P S D I D I S R S C K P K P I G K L A R E I G L L S E E V E L Y G E T K A K V L L S A L E R L K H R P D G K Y V V V T G I T P T P L G E G K S T T T I G L V Q A L G A H L Y Q N V F A C V R Q P S Q G P T F G I K G G A A G G G Y S Q V I P M E E F N L H L T G D I H A I T A A N N L V A A A I D A R I F H E L T Q T D K A L F N R L V P S V N G V R R F S D I Q I R R L K R L G I E K T D P T T L T D E E I N R F A R L D I D P E T I T W Q R V L D T N D R F L R K I T I G Q A P T E K G H T R T A Q F D I S V A S E I M A V L A L T T S L E D M R E R L G K M V V A S S K K G E P V S A E D L G V S G A L T V L M K D A I K P N L M Q T L E G T P V F V H A G P F A N I A H G N S S I I A D Q I A L K L V G P E G F V V T E A G F G A D I G M E K F F N I K C R Y S G L C P H V V V L V A T V R A L K M H G G G P T V T A G L P L P K A Y I Q E N L E L V E K G F S N L K K Q I E N A R M F G I P V V V A V N A F K T D T E S E L D L I S R L S R E H G A F D A V K C T H W A E G G K G A L A L A Q A V Q R A A Q A P S S F Q L L Y D L K L P V E D K I R I I A Q K I Y G A D D I E L L P E A Q H K A E V Y T K Q G F G N L P I C M A K T H L S L S H N P E Q K G V P T G F I L P I R D I R A S V G A G F L Y P L V G T M S T M P G L P T R P C F Y D I D L D P E T E Q V N G L F
- Accession
- P11586
- Protein length
- Full Length
- Predicted molecular weight
- 128.59 kDa
- Amino acids
- 1 to 935
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Trifunctional enzyme that catalyzes the interconversion of three forms of one-carbon-substituted tetrahydrofolate: (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate, 5,10-methenyltetrahydrofolate and (6S)-10-formyltetrahydrofolate (PubMed:10828945, PubMed:18767138, PubMed:1881876). These derivatives of tetrahydrofolate are differentially required in nucleotide and amino acid biosynthesis, (6S)-10-formyltetrahydrofolate being required for purine biosynthesis while (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate is used for serine and methionine biosynthesis for instance (PubMed:18767138, PubMed:25633902).
- Sequence similarities
In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MTHFD1 protein also known as C1-THF synthase is a trifunctional enzyme involved in the folate metabolism. It has a mass of approximately 102 kDa and functions within the cytoplasm. MTHFD1 catalyzes three sequential activities: methylene-THF dehydrogenase methenyl-THF cyclohydrolase and formyl-THF synthetase. These enzymatic activities are fundamental for cellular one-carbon metabolism. This protein is widely expressed in various tissues including liver and fetal tissues reflecting its importance in cellular metabolism.
- Biological function summary
MTHFD1 plays a central role in folate-mediated one-carbon transfer reactions. It connects the cytoplasmic folate cycle to nucleotide biosynthesis and amino acid metabolism. As a multifunctional enzyme it is known to integrate with the cytosolic one-carbon metabolic complex allowing it to coordinate closely with other enzymes in the cycle. This function supports the synthesis of purines and thymidylate which are essential building blocks for DNA and RNA.
- Pathways
MTHFD1 integrates into the folate and methionine cycles important parts of cellular metabolism. It collaborates with enzymes such as SHMT1 in the biosynthesis of nucleotides. The folate cycle in which MTHFD1 participates provides one-carbon units necessary for methylation reactions and nucleotide synthesis playing a part in maintaining genomic stability. This partnership with the methionine cycle further indicates its influence on cellular methylation patterns impacting gene expression and protein function.
- Associated diseases and disorders
MTHFD1 has associations with certain conditions notably neural tube defects and cardiovascular disease. Mutations or polymorphisms in the MTHFD1 gene can disrupt the folate and methionine cycles leading to disturbed one-carbon metabolism. In neural tube defects low activity of MTHFD1 correlates with insufficient nucleotide biosynthesis and improper DNA methylation. In cardiovascular disease MTHFD1 interacts with homocysteine metabolism through its role in the methylation cycle influencing levels of this risk-associated amino acid.
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1 product image
SDS-PAGE - Recombinant Human MTHFD1 protein (ab116852)
12.5% SDS-PAGE showing ab116852 at approximately 128.59kDa; stained with Coomassie Blue.
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