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AB202178

Recombinant Human MTHFD2 protein (His tag N-Terminus)

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Recombinant Human MTHFD2 protein (His tag N-Terminus) is a Human Full Length protein, in the 30 to 350 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

NMDMC, MTHFD2

1 Images
SDS-PAGE - Recombinant Human MTHFD2 protein (AB202178)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human MTHFD2 protein (AB202178)

15% SDS-PAGE analysis of ab202178 (3 μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P13995

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSLAAVRNEAVVISGRKLAQQIKQEVRQEVEEWVASGNKRPHLSVILVGENPASHSYVLNKTRAAAVVGINSETIMKPASISEEELLNLINKLNNDDNVDGLLVQLPLPEHIDERRICNAVSPDKDVDGFHVINVGRMCLDQYSMLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHTDGAHERPGGDATVTISHRYTPKEQLKKHTILADIVISAAGIPNLITADMIKEGAAVIDVGINRVHDPVTAKPKLVGDVDFEGVRQKAGYITPVPGGVGPMTVAMLMKNTIIAAKKVLRLEEREVLKSKELGVATN","proteinLength":"Full Length","predictedMolecularWeight":"37.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":350,"aminoAcidStart":30,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P13995","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MTHFD2 also known as methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 is an enzyme with a mass of approximately 36 kDa. It catalyzes the conversion of methylenetetrahydrofolate to methenyltetrahydrofolate using NADP+ as a cofactor. MTHFD2 is a mitochondrial protein and is highly expressed in embryonic tissues and various cancers while showing low expression in normal adult tissues. This differential expression makes it an interesting target for cancer research.
Biological function summary

Methylenetetrahydrofolate dehydrogenase plays an important role in folate metabolism contributing to the one-carbon metabolic pathway. MTHFD2 is part of the tetrahydrofolate (THF) complex critical for DNA synthesis and repair. The enzyme supports the production of nucleotides and amino acids by providing necessary one-carbon units linking it to rapid cellular proliferation as seen in cancerous cells.

Pathways

MTHFD2 is key in connecting the folate-mediated one-carbon metabolic network to the central metabolism. It is involved in the metabolic pathways that synthesize purines and thymidylate. In these networks MTHFD2 interacts with proteins such as SHMT2 (serine hydroxymethyltransferase 2) facilitating serine to glycine conversion which is important for the provision of one-carbon donors for methylation reactions.

The upregulation of MTHFD2 strongly associates with various cancers including breast and colorectal cancers. The involvement of MTHFD2 in these cancers indicates its potential as a biomarker and therapeutic target. Through cancer-related pathways MTHFD2 shows a connection to enzymes such as ALDH1L2 (aldehyde dehydrogenase 1 family member L2) which also participates in folate metabolism and influences cancer progression.
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Specifications

Form

Liquid

Additional notes

ab202178 was purified using conventional chromatography techniques.

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General info

Function

Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency.

Sequence similarities

Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.

Subcellular localisation

Mitochondrion

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Product protocols

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Target data

Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency.
See full target information MTHFD2
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