Recombinant Human MTHFD2 protein is a Human Full Length protein, in the 30 to 350 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S L A A V R N E A V V I S G R K L A Q Q I K Q E V R Q E V E E W V A S G N K R P H L S V I L V G E N P A S H S Y V L N K T R A A A V V G I N S E T I M K P A S I S E E E L L N L I N K L N N D D N V D G L L V Q L P L P E H I D E R R I C N A V S P D K D V D G F H V I N V G R M C L D Q Y S M L P A T P W G V W E I I K R T G I P T L G K N V V V A G R S K N V G M P I A M L L H T D G A H E R P G G D A T V T I S H R Y T P K E Q L K K H T I L A D I V I S A A G I P N L I T A D M I K E G A A V I D V G I N R V H D P V T A K P K L V G D V D F E G V R Q K A G Y I T P V P G G V G P M T V A M L M K N T I I A A K K V L R L E E R E V L K S K E L G V A T N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency.
Alternative names
NMDMC, MTHFD2
Recommended products
Recombinant Human MTHFD2 protein is a Human Full Length protein, in the 30 to 350 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P13995-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S L A A V R N E A V V I S G R K L A Q Q I K Q E V R Q E V E E W V A S G N K R P H L S V I L V G E N P A S H S Y V L N K T R A A A V V G I N S E T I M K P A S I S E E E L L N L I N K L N N D D N V D G L L V Q L P L P E H I D E R R I C N A V S P D K D V D G F H V I N V G R M C L D Q Y S M L P A T P W G V W E I I K R T G I P T L G K N V V V A G R S K N V G M P I A M L L H T D G A H E R P G G D A T V T I S H R Y T P K E Q L K K H T I L A D I V I S A A G I P N L I T A D M I K E G A A V I D V G I N R V H D P V T A K P K L V G D V D F E G V R Q K A G Y I T P V P G G V G P M T V A M L M K N T I I A A K K V L R L E E R E V L K S K E L G V A T N
- Accession
- P13995
- Protein length
- Full Length
- Predicted molecular weight
- 37.2 kDa
- Amino acids
- 30 to 350
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab202178 was purified using conventional chromatography techniques.
General info
- Function
Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency.
- Sequence similarities
Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
- Subcellular localisation
- Mitochondrion
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MTHFD2 also known as methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 is an enzyme with a mass of approximately 36 kDa. It catalyzes the conversion of methylenetetrahydrofolate to methenyltetrahydrofolate using NADP+ as a cofactor. MTHFD2 is a mitochondrial protein and is highly expressed in embryonic tissues and various cancers while showing low expression in normal adult tissues. This differential expression makes it an interesting target for cancer research.
- Biological function summary
Methylenetetrahydrofolate dehydrogenase plays an important role in folate metabolism contributing to the one-carbon metabolic pathway. MTHFD2 is part of the tetrahydrofolate (THF) complex critical for DNA synthesis and repair. The enzyme supports the production of nucleotides and amino acids by providing necessary one-carbon units linking it to rapid cellular proliferation as seen in cancerous cells.
- Pathways
MTHFD2 is key in connecting the folate-mediated one-carbon metabolic network to the central metabolism. It is involved in the metabolic pathways that synthesize purines and thymidylate. In these networks MTHFD2 interacts with proteins such as SHMT2 (serine hydroxymethyltransferase 2) facilitating serine to glycine conversion which is important for the provision of one-carbon donors for methylation reactions.
- Associated diseases and disorders
The upregulation of MTHFD2 strongly associates with various cancers including breast and colorectal cancers. The involvement of MTHFD2 in these cancers indicates its potential as a biomarker and therapeutic target. Through cancer-related pathways MTHFD2 shows a connection to enzymes such as ALDH1L2 (aldehyde dehydrogenase 1 family member L2) which also participates in folate metabolism and influences cancer progression.
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1 product image
SDS-PAGE - Recombinant Human MTHFD2 protein (ab202178)
15% SDS-PAGE analysis of ab202178 (3 μg).
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