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AB104635

Recombinant Human MTHFS protein

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Recombinant Human MTHFS protein is a Human Full Length protein, in the 1 to 203 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

5-formyltetrahydrofolate cyclo-ligase, MTHFS, Methenyl-THF synthetase

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SDS-PAGE - Recombinant Human MTHFS protein (AB104635)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human MTHFS protein (AB104635)

15% SDS-PAGE analysis of 3μg ab104635.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P49914

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.316% Tris HCl, 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAAAAVSSAKRSLRGELKQRLRAMSAEERLRQSRVLSQKVIAHSEYQKSKRISIFLSMQDEIETEEIIKDIFQRGKICFIPRYRFQSNHMDMVRIESPEEISLLPKTSWNIPQPGEGDVREEALSTGGLDLIFMPGLGFDKHGNRLGRGKGYYDAYLKRCLQHQEVKPYTLALAFKEQICLQVPVNENDMKVDEVLYEDSSTA","proteinLength":"Full Length","predictedMolecularWeight":"25.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":203,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P49914","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MTHFS also known as methenyltetrahydrofolate synthetase is an enzyme that plays an important role in folate metabolism. It catalyzes the conversion of 5-formyltetrahydrofolate (5-formyl-THF) to 510-methenyltetrahydrofolate (510-methenyl-THF). MTHFS is a protein with a molecular mass of approximately 23 kDa. Expression occurs in various tissues with significant presence in the liver where folate-mediated one-carbon metabolism is important for various cellular processes.
Biological function summary

MTHFS impacts the cellular folate pool by regulating the balance of folate derivatives required for nucleotide biosynthesis and amino acid metabolism. It does not function as part of a larger protein complex but exerts its effects by modulating the availability of different folate forms. This balance is essential for the synthesis of purines and thymidylate key building blocks of DNA and RNA influencing cell proliferation.

Pathways

MTHFS plays a role in the folate cycle and the related one-carbon metabolism pathway. These pathways are critical for DNA synthesis and repair as well as methylation reactions. MTHFS interacts indirectly with enzymes such as dihydrofolate reductase (DHFR) and serine hydroxymethyltransferase (SHMT) which are also key players in these pathways. The precise regulation of these pathways reflects the importance of folate in maintaining cellular health and function.

Impaired MTHFS function can lead to disruptions in folate metabolism contributing to the development of conditions such as neural tube defects and certain types of anemia. Research suggests a link between altered MTHFS activity and increased risk of these disorders. Its relationship with other proteins involved in folate metabolism such as folate receptor 1 (FOLR1) highlights the broader impact of disrupted folate metabolism on disease mechanisms.
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Specifications

Form

Liquid

Additional notes

ab104635 is purified using conventional chromatography techniques.

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General info

Function

Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. Catalyzes the irreversible conversion of 5-formyltetrahydrofolate (5-FTHF) to yield 5,10-methenyltetrahydrofolate.

Sequence similarities

Belongs to the 5-formyltetrahydrofolate cyclo-ligase family.

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Product protocols

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Target data

Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. Catalyzes the irreversible conversion of 5-formyltetrahydrofolate (5-FTHF) to yield 5,10-methenyltetrahydrofolate.
See full target information MTHFS
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