Recombinant Human mtTFA protein is a Human Full Length protein, in the 43 to 246 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
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Publications
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- EMBO molecular medicine 15:e165812023LONP1 targets HMGCS2 to protect mitochondrial function and attenuate chronic kidney disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMi Bai,Mengqiu Wu,Mingzhu Jiang,Jia He,Xu Deng,Shuang Xu,Jiaojiao Fan,Mengqiu Miao,Ting Wang,Yuting Li,Xiaowen Yu,Lin Wang,Yue Zhang,Songming Huang,Li Yang,Zhanjun Jia,Aihua ZhangPubMed 36629048
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M S S V L A S C P K K P V S S Y L R F S K E Q L P I F K A Q N P D A K T T E L I R R I A Q R W R E L P D S K K K I Y Q D A Y R A E W Q V Y K E E I S R F K E Q L T P S Q I M S L E K E I M D K H L K R K A M T K K K E L T L L G K P K R P R S A Y N V Y V A E R F Q E A K G D S P Q E K L K T V K E N W K N L S D S E K E L Y I Q H A K E D E T R Y H N E M K S W E E Q M I E V G R K D L L R R T I K K Q R K Y G A E E C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation (PubMed:29445193, PubMed:32183942). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA (PubMed:29149603). In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (PubMed:20410300). Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase (PubMed:22037172). Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites (PubMed:22037172). Is able to unwind DNA (PubMed:22037172). Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes (PubMed:1737790). Required for maintenance of normal levels of mitochondrial DNA (PubMed:19304746, PubMed:22841477). May play a role in organizing and compacting mitochondrial DNA (PubMed:22037171).
Alternative names
TCF6, TCF6L2, TFAM, mtTFA, Mitochondrial transcription factor 1, Transcription factor 6, Transcription factor 6-like 2, MtTF1, TCF-6
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Recombinant Human mtTFA protein is a Human Full Length protein, in the 43 to 246 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q00059-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.316% Tris HCl, 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M S S V L A S C P K K P V S S Y L R F S K E Q L P I F K A Q N P D A K T T E L I R R I A Q R W R E L P D S K K K I Y Q D A Y R A E W Q V Y K E E I S R F K E Q L T P S Q I M S L E K E I M D K H L K R K A M T K K K E L T L L G K P K R P R S A Y N V Y V A E R F Q E A K G D S P Q E K L K T V K E N W K N L S D S E K E L Y I Q H A K E D E T R Y H N E M K S W E E Q M I E V G R K D L L R R T I K K Q R K Y G A E E C
- Accession
- Q00059
- Protein length
- Full Length
- Predicted molecular weight
- 26.6 kDa
- Amino acids
- 43 to 246
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab103784 was purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
General info
- Function
Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation (PubMed:29445193, PubMed:32183942). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA (PubMed:29149603). In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (PubMed:20410300). Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase (PubMed:22037172). Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites (PubMed:22037172). Is able to unwind DNA (PubMed:22037172). Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes (PubMed:1737790). Required for maintenance of normal levels of mitochondrial DNA (PubMed:19304746, PubMed:22841477). May play a role in organizing and compacting mitochondrial DNA (PubMed:22037171).
- Post-translational modifications
Phosphorylation by PKA within the HMG box 1 impairs DNA binding and promotes degradation by the AAA+ Lon protease.
- Subcellular localisation
- Mitochondrion, Mitochondrion matrix, Mitochondrion nucleoid
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MtTFA also known as mitochondrial transcription factor A or TFAM is an important element in mitochondrial DNA regulation. It plays a pivotal role in maintaining mitochondrial genome integrity and ensuring proper mitochondrial function. This protein weighing approximately 25 kDa is expressed within the mitochondria. It binds to the mitochondrial DNA facilitating the transcription and replication processes ensuring the correct functioning of cellular energy metabolism.
- Biological function summary
MtTFA is essential for mitochondrial DNA packaging and transcriptional activation. It acts as a binding factor that organizes the mtDNA into a nucleoid-like structure providing protection and stability. MtTFA functions as part of a larger multi-protein complex that includes mitochondrial RNA polymerase ensuring proper transcription of mitochondrial genes. These activities are critical for normal energy production and mitochondrial biogenesis.
- Pathways
The mtTFA protein is integral to the oxidative phosphorylation and mitochondrial biogenesis pathways. It orchestrates the expression of genes critical for these pathways alongside related proteins like NRF2 and PGC-1α. MtTFA ensures effective mitochondrial function by modulating the transcription of key mitochondrial-encoded components necessary for electron transport and ATP synthesis thereby sustaining cellular energy balance.
- Associated diseases and disorders
MtTFA dysregulation is associated with mitochondrial diseases and neurodegenerative disorders. Abnormal mtTFA activity disrupts mitochondrial DNA maintenance potentially leading to conditions like mitochondrial myopathy. It is also linked with proteins such as POLG which are involved in mitochondrial DNA replication. Understanding mtTFA's role in these pathologies highlights its importance as a target in therapeutic strategies aimed at restoring mitochondrial function and preventing disease progression.
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SDS-PAGE - Recombinant Human mtTFA protein (ab103784)
15% SDS-PAGE analysis of 3μg ab103784.
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