Products
Sample Prep & Detection Kits
Conjugation kitsPurification kitsSample preparation kitsChromogen kitsIHC kitsChIP kitsAccessory Reagents & Controls
Accessory reagents & controlsBiochemicals
BiochemicalsProteins and Peptides
Proteins and peptidesOur latest ELISA kit: Human Tau (phospho T217) - Intracellular
Highly sensitive kit offering the most promising biomarkers for Alzheimer’s disease diagnostics. Learn about all product ranges with our product overviews.
Resources
Featured events
Make new connections at our global events.
Support
Our programs
New Lab Program
Get a head start with our exclusive new lab discount. Enjoy 20% off and free shipping for three months.
New Biotech Program
Just starting out? Get 15% off and free shipping to your lab for six months.
Product promise
Peace of mind that all products perform as stated.
Product reviews
Leave reviews, get rewarded and help your community.
Trial program
Try untested species and applications to earn money off your next order.
Recombinant Human/Murine/Rat BDNF protein (Active) is a Human Full Length protein, in the 129 to 247 aa range, expressed in Escherichia coli, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, WB, CellAct.
Alternative names=Brain-derived neurotrophic factor, BDNF, Abrineurin, BDNF
- Purity
>98% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, HPLC, WB, CellAct
- Biologically active
Yes
- Sequence
M H S D P A R R G E L S V C D S I S E W V T A A D K K T A V D M S G G T V T V L E K V P V S K G Q L K Q Y F Y E T K C N P M G Y T K E G C R G I D K R H W N S Q C R T T Q S Y V R A L T M D S K K R I G W R F I R I D T S C V C T L T I K R G R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes Ab9794 can be used as a WB positive control in conjunction with ab6201. |
Application CellAct | Reactivity Reacts | Dilution info 0.5-1 µg/mL | Notes The expected ED50 is 0.5-1.0 µg/ml for its ability to stimulate the proliferation of Rat C6 cells. |
Images
Recommended products
Recombinant Human/Murine/Rat BDNF protein (Active) is a Human Full Length protein, in the 129 to 247 aa range, expressed in Escherichia coli, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, WB, CellAct.
Alternative names=Brain-derived neurotrophic factor, BDNF, Abrineurin, BDNF
Key facts
- Purity
>98% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, HPLC, WB, CellAct
- Biologically active
Yes
- Biological activity
Determined by its ability to bind recombinant human TrkB Fc Chimera in a functional ELISA.
The active form of BDNF is a 27.0 kDa, dimer formed by two identical 119 amino acid subunits that is held together by strong hydrophobic interactions.
- Predicted molecular weight
27 kDa
- Amino acids
129 to 247
- Sequence
M H S D P A R R G E L S V C D S I S E W V T A A D K K T A V D M S G G T V T V L E K V P V S K G Q L K Q Y F Y E T K C N P M G Y T K E G C R G I D K R H W N S Q C R T T Q S Y V R A L T M D S K K R I G W R F I R I D T S C V C T L T I K R G R
- Accession
- P23560-1
- Protein length
Full Length
- Animal free
No
- Nature
Recombinant
- Species
Human
- Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use.
- Concentration
Specifications
- Form
Lyophilized
- Additional notes
> 98% pure HPLC analyses.
General info
- Function
Important signaling molecule that activates signaling cascades downstream of NTRK2 (PubMed:11152678). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
- Sequence similarities
Belongs to the NGF-beta family.
- Post-translational modifications
BDNF precursor form
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com