Recombinant Human/Murine/Rat BDNF protein (Active) is a Human Full Length protein, in the 129 to 247 aa range, expressed in Escherichia coli, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, WB, CellAct.
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- Brain plasticity (Amsterdam, Netherlands) 8:5-182022High Intensity Acute Aerobic Exercise Elicits Alterations in Circulating and Skeletal Muscle Tissue Expression of Neuroprotective Exerkines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCorey E Mazo,Edwin R Miranda,James Shadiow,Michael Vesia,Jacob M HausPubMed 36448040
- International journal of molecular sciences 23:2022High-Contrast Stimulation Potentiates the Neurotrophic Properties of Müller Cells and Suppresses Their Pro-Inflammatory Phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiloslav Zloh,Patrik Kutilek,Andrea StofkovaPubMed 35955747
- Frontiers in neuroscience 15:7253982021Brain-Derived Neurotrophic Factor Secreting Human Mesenchymal Stem Cells Improve Outcomes in Rett Syndrome Mouse Models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyo Jeong Kim,Delger Bayarsaikhan,Jaesuk Lee,Govigerel Bayarsaikhan,Bonghee LeePubMed 34690674
- Bulletin of experimental biology and medicine 169:701-7062020Brain-Derived Neurotrophic Factor (BDNF) As a Regulator of Apoptosis under Conditions of Focal Experimental Stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS G Kalinichenko et. al.PubMed 32990850
- Biomedicines 8:2020Three-Dimensional Model of Dorsal Root Ganglion Explant as a Method of Studying Neurotrophic Factors in Regenerative Medicine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPolina Klimovich et. al.PubMed 32138155
- Experimental and therapeutic medicine 18:3837-38442019Exogenous brain-derived neurotrophic factor attenuates neuronal apoptosis and neurological deficits after subarachnoid hemorrhage in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuayun Chen et. al.PubMed 31616511
- iScience 19:927-9392019Linking NMDA Receptor Synaptic Retention to Synaptic Plasticity and Cognition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuca Franchini,Jennifer Stanic,Luisa Ponzoni,Manuela Mellone,Nicolò Carrano,Stefano Musardo,Elisa Zianni,Guendalina Olivero,Elena Marcello,Anna Pittaluga,Mariaelvina Sala,Camilla Bellone,Claudia Racca,Monica Di Luca,Fabrizio GardoniPubMed 31518901
- Toxicological sciences : an official journal of th :2019Prenatal Dexamethasone Exposure Induced Alterations in Neurobehavior and Hippocampal Glutamatergic System Balance in Female Rat Offspring.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSongqiang Huang et. al.PubMed 31518422
- Journal of cellular biochemistry 120:18771-187812019The inhibition of BDNF/TrkB/PI3K/Akt signal mediated by AG1601 promotes apoptosis in malignant glioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongqiang Yin et. al.PubMed 31219215
- Frontiers in cellular neuroscience 13:1152019mTORC1 Signaling Is Palmitoylation-Dependent in Hippocampal Neurons and Non-neuronal Cells and Involves Dynamic Palmitoylation of LAMTOR1 and mTOR.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaun S Sanders et. al.PubMed 31001086
Key facts
- Purity
- >98% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, HPLC, WB, CellAct
- Biologically active
- Yes
Amino acid sequence
M H S D P A R R G E L S V C D S I S E W V T A A D K K T A V D M S G G T V T V L E K V P V S K G Q L K Q Y F Y E T K C N P M G Y T K E G C R G I D K R H W N S Q C R T T Q S Y V R A L T M D S K K R I G W R F I R I D T S C V C T L T I K R G R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab9794 can be used as a WB positive control in conjunction with ab6201. |
Application CellAct | Reactivity Reacts | Dilution info 0.5-1 µg/mL | Notes The expected ED50 is 0.5-1.0 µg/ml for its ability to stimulate the proliferation of Rat C6 cells. |
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Target data
Function
The protein expressed by the BDNF gene is a critical signaling molecule that activates pathways downstream of NTRK2, as well as the heterodimeric receptor formed by NGFR and SORCS2. During development, it aids the survival and differentiation of specific neuronal populations in both the peripheral and central nervous systems. It is involved in axonal growth, pathfinding, and modulation of dendritic growth and morphology. In adult synapses, BDNF is a key regulator of synaptic transmission and plasticity in various CNS regions, contributing to processes like long-term potentiation, long-term depression, some short-term synaptic plasticity forms, and the homeostatic regulation of intrinsic neuronal excitability. Signaling through NGFR and SORCS2 is linked to synaptic plasticity and long-term depression, while its binding to these receptors also promotes neuronal apoptosis. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
Neurotrophic factor BDNF precursor form, proBDNF, Abrineurin, Brain-derived neurotrophic factor, BDNF
Recommended products
Recombinant Human/Murine/Rat BDNF protein (Active) is a Human Full Length protein, in the 129 to 247 aa range, expressed in Escherichia coli, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, WB, CellAct.
Key facts
- Purity
- >98% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, HPLC, WB, CellAct
- Biologically active
- Yes
- Biological activity
- Determined by its ability to bind recombinant human TrkB Fc Chimera in a functional ELISA.
The active form of BDNF is a 27.0 kDa, dimer formed by two identical 119 amino acid subunits that is held together by strong hydrophobic interactions.
- Accession
- P23560-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute in water
- Concentration
Sequence info
Amino acid sequence
- M H S D P A R R G E L S V C D S I S E W V T A A D K K T A V D M S G G T V T V L E K V P V S K G Q L K Q Y F Y E T K C N P M G Y T K E G C R G I D K R H W N S Q C R T T Q S Y V R A L T M D S K K R I G W R F I R I D T S C V C T L T I K R G R
- Accession
- P23560
- Protein length
- Full Length
- Predicted molecular weight
- 27 kDa
- Amino acids
- 129 to 247
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
> 98% pure HPLC analyses.
General info
- Function
The protein expressed by the BDNF gene is a critical signaling molecule that activates pathways downstream of NTRK2, as well as the heterodimeric receptor formed by NGFR and SORCS2. During development, it aids the survival and differentiation of specific neuronal populations in both the peripheral and central nervous systems. It is involved in axonal growth, pathfinding, and modulation of dendritic growth and morphology. In adult synapses, BDNF is a key regulator of synaptic transmission and plasticity in various CNS regions, contributing to processes like long-term potentiation, long-term depression, some short-term synaptic plasticity forms, and the homeostatic regulation of intrinsic neuronal excitability. Signaling through NGFR and SORCS2 is linked to synaptic plasticity and long-term depression, while its binding to these receptors also promotes neuronal apoptosis.
This supplementary information is collated from multiple sources and compiled automatically.- Sequence similarities
Belongs to the NGF-beta family.
- Post-translational modifications
Neurotrophic factor BDNF precursor form
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Recombinant Human/Murine/Rat BDNF protein (Active) (ab9794)
All lanes: Rabbit Anti-BDNF antibody at 1 µg/mL
All lanes: Western blot - Recombinant Human/Murine/Rat BDNF protein (Active) (ab9794) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 4min
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