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Recombinant Human/Murine/Rat BDNF protein (Active) is a Human Full Length protein, in the 129 to 247 aa range, expressed in Escherichia coli, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, WB, CellAct.

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Purity

>98% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, HPLC, WB, CellAct

Biologically active

Yes

Sequence

M H S D P A R R G E L S V C D S I S E W V T A A D K K T A V D M S G G T V T V L E K V P V S K G Q L K Q Y F Y E T K C N P M G Y T K E G C R G I D K R H W N S Q C R T T Q S Y V R A L T M D S K K R I G W R F I R I D T S C V C T L T I K R G R

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Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
HPLC
Reactivity
Reacts
Dilution info
-
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

Ab9794 can be used as a WB positive control in conjunction with ab6201.

Application
CellAct
Reactivity
Reacts
Dilution info
0.5-1 µg/mL
Notes

The expected ED50 is 0.5-1.0 µg/ml for its ability to stimulate the proliferation of Rat C6 cells.

Images

Target data

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Recombinant Human/Murine/Rat BDNF protein (Active) is a Human Full Length protein, in the 129 to 247 aa range, expressed in Escherichia coli, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, WB, CellAct.

Key facts

Purity

>98% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Applications

SDS-PAGE, HPLC, WB, CellAct

Biological activity

Determined by its ability to bind recombinant human TrkB Fc Chimera in a functional ELISA.

The active form of BDNF is a 27.0 kDa, dimer formed by two identical 119 amino acid subunits that is held together by strong hydrophobic interactions.

Predicted molecular weight

27 kDa

Amino acids

129 to 247

Sequence

M H S D P A R R G E L S V C D S I S E W V T A A D K K T A V D M S G G T V T V L E K V P V S K G Q L K Q Y F Y E T K C N P M G Y T K E G C R G I D K R H W N S Q C R T T Q S Y V R A L T M D S K K R I G W R F I R I D T S C V C T L T I K R G R

Accession
P23560-1
Protein length

Full Length

Animal free

No

Nature

Recombinant

Species

Human

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use.

Concentration
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Specifications

Form

Lyophilized

Additional notes

> 98% pure HPLC analyses.

General info

Function

Important signaling molecule that activates signaling cascades downstream of NTRK2 (PubMed:11152678). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

Sequence similarities

Belongs to the NGF-beta family.

Post-translational modifications

BDNF precursor form

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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