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Recombinant Human/Murine/Rat BDNF protein (Active) is a Human Full Length protein, in the 129 to 247 aa range, expressed in Escherichia coli, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, WB, CellAct.
Alternative names=Brain-derived neurotrophic factor, BDNF, Abrineurin, BDNF
>98% SDS-PAGE
< 1 EU/µg
Escherichia coli
Tag free
SDS-PAGE, HPLC, WB, CellAct
Yes
M H S D P A R R G E L S V C D S I S E W V T A A D K K T A V D M S G G T V T V L E K V P V S K G Q L K Q Y F Y E T K C N P M G Y T K E G C R G I D K R H W N S Q C R T T Q S Y V R A L T M D S K K R I G W R F I R I D T S C V C T L T I K R G R
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes Ab9794 can be used as a WB positive control in conjunction with ab6201. |
Application CellAct | Reactivity Reacts | Dilution info 0.5-1 µg/mL | Notes The expected ED50 is 0.5-1.0 µg/ml for its ability to stimulate the proliferation of Rat C6 cells. |
Recombinant Human/Murine/Rat BDNF protein (Active) is a Human Full Length protein, in the 129 to 247 aa range, expressed in Escherichia coli, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, WB, CellAct.
Alternative names=Brain-derived neurotrophic factor, BDNF, Abrineurin, BDNF
>98% SDS-PAGE
< 1 EU/µg
Escherichia coli
Tag free
SDS-PAGE, HPLC, WB, CellAct
Yes
Determined by its ability to bind recombinant human TrkB Fc Chimera in a functional ELISA.
The active form of BDNF is a 27.0 kDa, dimer formed by two identical 119 amino acid subunits that is held together by strong hydrophobic interactions.
27 kDa
129 to 247
M H S D P A R R G E L S V C D S I S E W V T A A D K K T A V D M S G G T V T V L E K V P V S K G Q L K Q Y F Y E T K C N P M G Y T K E G C R G I D K R H W N S Q C R T T Q S Y V R A L T M D S K K R I G W R F I R I D T S C V C T L T I K R G R
Full Length
No
Recombinant
Human
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use.
Lyophilized
> 98% pure HPLC analyses.
Important signaling molecule that activates signaling cascades downstream of NTRK2 (PubMed:11152678). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
Belongs to the NGF-beta family.
BDNF precursor form
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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