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Recombinant Human/Murine/Rat BDNF protein (Active) is a Human Full Length protein, in the 129 to 247 aa range, expressed in Escherichia coli, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, WB, CellAct.
Alternative names=Brain-derived neurotrophic factor, BDNF, Abrineurin, BDNF
- Purity
>98% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, HPLC, WB, CellAct
- Biologically active
Yes
- Sequence
M H S D P A R R G E L S V C D S I S E W V T A A D K K T A V D M S G G T V T V L E K V P V S K G Q L K Q Y F Y E T K C N P M G Y T K E G C R G I D K R H W N S Q C R T T Q S Y V R A L T M D S K K R I G W R F I R I D T S C V C T L T I K R G R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes Ab9794 can be used as a WB positive control in conjunction with ab6201. |
Application CellAct | Reactivity Reacts | Dilution info 0.5-1 µg/mL | Notes The expected ED50 is 0.5-1.0 µg/ml for its ability to stimulate the proliferation of Rat C6 cells. |
Images
Target data
Function
Important signaling molecule that activates signaling cascades downstream of NTRK2 (PubMed:11152678). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.BDNF precursor formImportant signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2 (PubMed:24908487, PubMed:29909994). Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse (By similarity).
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Recombinant Human/Murine/Rat BDNF protein (Active) is a Human Full Length protein, in the 129 to 247 aa range, expressed in Escherichia coli, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, WB, CellAct.
Alternative names=Brain-derived neurotrophic factor, BDNF, Abrineurin, BDNF
Key facts
- Purity
>98% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, HPLC, WB, CellAct
- Biologically active
Yes
- Biological activity
Determined by its ability to bind recombinant human TrkB Fc Chimera in a functional ELISA.
The active form of BDNF is a 27.0 kDa, dimer formed by two identical 119 amino acid subunits that is held together by strong hydrophobic interactions.
- Predicted molecular weight
27 kDa
- Amino acids
129 to 247
- Sequence
M H S D P A R R G E L S V C D S I S E W V T A A D K K T A V D M S G G T V T V L E K V P V S K G Q L K Q Y F Y E T K C N P M G Y T K E G C R G I D K R H W N S Q C R T T Q S Y V R A L T M D S K K R I G W R F I R I D T S C V C T L T I K R G R
- Accession
- P23560-1
- Protein length
Full Length
- Animal free
No
- Nature
Recombinant
- Species
Human
- Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use.
- Concentration
Specifications
- Form
Lyophilized
- Additional notes
> 98% pure HPLC analyses.
General info
- Function
Important signaling molecule that activates signaling cascades downstream of NTRK2 (PubMed:11152678). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
- Sequence similarities
Belongs to the NGF-beta family.
- Post-translational modifications
BDNF precursor form
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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