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Recombinant Human MYBPC3 protein (His tag) is a Human Fragment protein, in the 1 to 328 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.

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Images

SDS-PAGE - Recombinant Human MYBPC3 protein (His tag) (AB225662), expandable thumbnail

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

Biologically active

No

Amino acid sequence

M P E P G K K P V S A F S K K P R S V E V A A G S P A V F E A E T E R A G V K V R W Q R G G S D I S A S N K Y G L A T E G T R H T L T V R E V G P A D Q G S Y A V I A G S S K V K F D L K V I E A E K A E P M L A P A P A P A E A T G A P G E A P A P A A E L G E S A P S P K G S S S A A L N G P T P G A P D D P I G L F V M R P Q D G E V T V G G S I T F S A R V A G A S L L K P P V V K W F K G K W V D L S S K V G Q H L Q L H D S Y D R A S K V Y L F E L H I T D A Q P A F T G S Y R C E V S T K D K F D C S N F N L T V H E A M G T G D L D L L S A F R R T S L A G G G R R I S D S H E D T G I L D F S S L L K K R D S F R T P R D S K L E A P A E E D V W E I L R Q A

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info
-
Notes

-

Target data

Function

Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.

Alternative names

Recommended products

Recombinant Human MYBPC3 protein (His tag) is a Human Fragment protein, in the 1 to 328 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE

Accession
Q14896-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

Sequence info

Amino acid sequence

M P E P G K K P V S A F S K K P R S V E V A A G S P A V F E A E T E R A G V K V R W Q R G G S D I S A S N K Y G L A T E G T R H T L T V R E V G P A D Q G S Y A V I A G S S K V K F D L K V I E A E K A E P M L A P A P A P A E A T G A P G E A P A P A A E L G E S A P S P K G S S S A A L N G P T P G A P D D P I G L F V M R P Q D G E V T V G G S I T F S A R V A G A S L L K P P V V K W F K G K W V D L S S K V G Q H L Q L H D S Y D R A S K V Y L F E L H I T D A Q P A F T G S Y R C E V S T K D K F D C S N F N L T V H E A M G T G D L D L L S A F R R T S L A G G G R R I S D S H E D T G I L D F S S L L K K R D S F R T P R D S K L E A P A E E D V W E I L R Q A

Accession
Q14896
Protein length

Fragment

Predicted molecular weight

50.8 kDa

Amino acids

1 to 328

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Liquid

General info

Function

Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.

Sequence similarities

Belongs to the immunoglobulin superfamily. MyBP family.

Post-translational modifications

Substrate for phosphorylation by PKA and PKC. Reversible phosphorylation appears to modulate contraction (By similarity).

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • SDS-PAGE - Recombinant Human MYBPC3 protein (His tag) (ab225662), expandable thumbnail

    SDS-PAGE - Recombinant Human MYBPC3 protein (His tag) (ab225662)

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab225662 with 5% enrichment gel and 15% separation gel.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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