Recombinant Human MYL12B protein is a Human Full Length protein, in the 1 to 172 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Publications
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- BMC research notes 12:7172019Contaminating reactivity of a monoclonal CCAAT/Enhancer Binding Protein β antibody in differentiating myoblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHamood AlSudais et. al.PubMed 31672165
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M S S K K A K T K T T K K R P Q R A T S N V F A M F D Q S Q I Q E F K E A F N M I D Q N R D G F I D K E D L H D M L A S L G K N P T D A Y L D A M M N E A P G P I N F T M F L T M F G E K L N G T D P E D V I R N A F A C F D E E A T G T I Q E D Y L R E L L T T M G D R F T D E E V D E L Y R E A P I D K K G N F N Y I E F T R I L K H G A K D K D D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion.
Alternative names
MRLC2, MYLC2B, MYL12B, Myosin regulatory light chain 12B, MLC-2A, Myosin regulatory light chain 20 kDa, Myosin regulatory light chain MRLC2, SHUJUN-1, MLC-2, MLC20
Recommended products
Recombinant Human MYL12B protein is a Human Full Length protein, in the 1 to 172 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- O14950-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 40% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M S S K K A K T K T T K K R P Q R A T S N V F A M F D Q S Q I Q E F K E A F N M I D Q N R D G F I D K E D L H D M L A S L G K N P T D A Y L D A M M N E A P G P I N F T M F L T M F G E K L N G T D P E D V I R N A F A C F D E E A T G T I Q E D Y L R E L L T T M G D R F T D E E V D E L Y R E A P I D K K G N F N Y I E F T R I L K H G A K D K D D
- Accession
- O14950
- Protein length
- Full Length
- Predicted molecular weight
- 22.3 kDa
- Amino acids
- 1 to 172
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab128438 was purified using conventional chromatography.
General info
- Function
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion.
- Post-translational modifications
Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is reduced following epigallocatechin-3-O-gallate treatment.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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Full details and terms and conditions can be found here:
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1 product image
SDS-PAGE - Recombinant Human MYL12B protein (ab128438)
15% SDS-PAGE analysis of 3μg ab128438.
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