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AB104014

Recombinant Human MYL9 protein (His tag N-Terminus)

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Recombinant Human MYL9 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 172 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec, WB.

View Alternative Names

MLC2, MRLC1, MYRL2, MYL9, Myosin regulatory light polypeptide 9, 20 kDa myosin light chain, MLC-2C, Myosin RLC, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1, LC20

2 Images
Western blot - Recombinant Human MYL9 protein (His tag N-Terminus) (AB104014)
  • WB

Unknown

Western blot - Recombinant Human MYL9 protein (His tag N-Terminus) (AB104014)

All lanes:

Western blot - Anti-MYL9 antibody (<a href='/en-us/products/primary-antibodies/myl9-antibody-ab64161'>ab64161</a>) at 1/2000 dilution

All lanes:

Western blot - Recombinant Human MYL9 protein (His tag N-Terminus) (ab104014) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 20 kDa

true

Exposure time: 4min

SDS-PAGE - Recombinant Human MYL9 protein (His tag N-Terminus) (AB104014)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MYL9 protein (His tag N-Terminus) (AB104014)

15% SDS-PAGE analysis of ab104014 (3 μg)

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, WB, SDS-PAGE

applications

Biologically active

No

Accession

P24844

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab104014 can be used as a WB positive control in conjunction with <a href='/en-us/products/primary-antibodies/myl9-antibody-ab64161'>ab64161</a>.</p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMSSKRAKAKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDHLRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKDD","proteinLength":"Full Length","predictedMolecularWeight":"21.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":172,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P24844","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

Purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.

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General info

Function

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion (PubMed : 11942626, PubMed : 2526655). In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation (By similarity).

Post-translational modifications

Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is required for myotube formation.

Subcellular localisation

Cytoskeleton

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Product protocols

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Target data

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion (PubMed : 11942626, PubMed : 2526655). In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation (By similarity).
See full target information MYL9
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 antibody [EPR13012(2)] - BSA and Azide free (AB236126)

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SDS-PAGE - Recombinant Human THTPA protein (His tag N-Terminus) (AB98233)

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Proteins & Peptides

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SDS-PAGE - Recombinant Human TNIP1 protein (His tag N-Terminus) (AB101108)

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Product promise

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