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AB84799

Recombinant human MYLK3/MLCK protein

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Recombinant human MYLK3/MLCK protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >75%, suitable for WB, FuncS.

View Alternative Names

MLCK, MYLK3, Myosin light chain kinase 3, Cardiac-MyBP-C-associated Ca/CaM kinase, Cardiac-MLCK

4 Images
Functional Studies - Recombinant human MYLK3/MLCK protein (AB84799)
  • FuncS

Unknown

Functional Studies - Recombinant human MYLK3/MLCK protein (AB84799)

The specific activity of MYLK3/MLCK (ab84799) was determined to be 11 nmol/min/mg as per activity assay protocol

Functional Studies - Recombinant human MYLK3/MLCK protein (AB84799)
  • FuncS

Unknown

Functional Studies - Recombinant human MYLK3/MLCK protein (AB84799)

Specific activity of ab84799

SDS-PAGE - Recombinant human MYLK3/MLCK protein (AB84799)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human MYLK3/MLCK protein (AB84799)

SDS PAGE analysis of ab84799

SDS-PAGE - Recombinant human MYLK3/MLCK protein (AB84799)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human MYLK3/MLCK protein (AB84799)

SDS Page analysis of ab84799

Key facts

Purity

>75% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, WB

applications

Biologically active

Yes

Biological activity

Specific activity: 9nmol/min/mg.

Accession

Q32MK0

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab60014 (Mouse MYL9 full length protein) can be utilized as a substrate for assessing Kinase activity

Protein previously labeled as MYLK3.

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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q32MK0","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility (By similarity).

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Post-translational modifications

Phosphorylated on serine residues.

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Product protocols

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Target data

Kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility (By similarity).
See full target information MYLK3
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Product promise

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