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AB191000

Recombinant human MYO3A protein

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Recombinant human MYO3A protein is a Human Fragment protein, in the 1 to 434 aa range, expressed in Baculovirus infected Sf9 cells, with >85%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Myosin-IIIa, MYO3A

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Functional Studies - Recombinant human MYO3A protein (AB191000)
  • FuncS

Supplier Data

Functional Studies - Recombinant human MYO3A protein (AB191000)

Kinase Assay : The specific activity of ab191000 was determined to be 4 nmol /min/mg.

Functional Studies - Recombinant human MYO3A protein (AB191000)
  • FuncS

Unknown

Functional Studies - Recombinant human MYO3A protein (AB191000)

The specific activity of MYO3A (ab191000) was determined to be 4.5 nmol/min/mg as per activity assay protocol

SDS-PAGE - Recombinant human MYO3A protein (AB191000)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human MYO3A protein (AB191000)

SDS PAGE analysis of ab191000

SDS-PAGE - Recombinant human MYO3A protein (AB191000)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human MYO3A protein (AB191000)

SDS-PAGE analysis of ab191000.

Key facts

Purity

>85% Densitometry

Affinity purified.

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The Specific activity of ab191000 was determined to be 4 nmol/min/mg.

Accession

Q8NEV4

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Sequence info

[{"linker":null,"sequence":"MFPLIGKTIIFDNFPDPSDTWEITETIGKGTYGKVFKVLNKKNGQKAAVKILDPIHDIDEEIEAEYNILKALSDHPNVVRFYGIYFKKDKVNGDKLWLVLELCSGGSVTDLVKGFLKRGERMSEPLIAYILHEALMGLQHLHNNKTIHRDVKGNNILLTTEGGVKLVDFGVSAQLTSTRHRRNTSVGTPFWMAPEVIACEQQLDTTYDARCDTWSLGITAIELGDGDPPLADLHPMRALFKIPRNPPPKLRQPELWSAEFNDFISKCLTKDYEKRPTVSELLQHKFITQIEGKDVMLQKQLTEFIGIHQCMGGTEKARRERIHTKKGNFNRPLISNLKDVDDLATLEILDENTVSEQLEKCYSRDQIYVYVGDILIALNPFQSLGLYSTKHSKLYIGSKRTASPPHIFAMADLGYQSMITYNSDQCIVISGESG","proteinLength":"Fragment","predictedMolecularWeight":"76 kDa","actualMolecularWeight":null,"aminoAcidEnd":434,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q8NEV4","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MYO3A also known as Myosin IIIA is motor protein with an approximate mass of 187 kDa. This protein belongs to the class of actin-based motor proteins characterized by ATPase activity facilitating the movement along actin filaments. MYO3A is expressed mainly in the sensory cells of the inner ear and retina playing a role in cellular processes that require specialized cellular projections like stereocilia and photoreceptor cells. Its ability to regulate actin filament dynamics is essential in these sensory tissues.
Biological function summary

MYO3A influences cellular activities through its role in cargo transport and maintaining the organization of actin structures. It operates as part of a protein complex that includes the actin-related protein Arp2/3 which collaborates to promote actin polymerization and branching. Additionally MYO3A interacts with other proteins such as espin-1 accentuating its function in the maintenance and elongation of actin protrusions. This interaction underlines its contribution to the structural integrity necessary for proper sensory cell function.

Pathways

MYO3A is significant in sensory transduction and cell morphogenesis pathways. Its interaction with actin and the Arp2/3 complex places MYO3A within the pathways involved in the regulation of actin cytoskeleton remodeling. MYO3A works closely with other proteins like harmonin critical for organizing the mechanotransductive hair bundle in the inner ear. These connections emphasize the involvement of MYO3A in pathways affecting the maintenance of cellular architecture and signal transmission.

MYO3A mutations are linked to hereditary deafness and Usher syndrome type 3 which involves auditory and visual impairments. In the context of these diseases MYO3A interacts with proteins like cadherin 23 important for hair cell function in the cochlea and usherin associated with retinal cells. These associations highlight MYO3A's importance in sensory cell function and its role in maintaining cellular structures that when disrupted can lead to sensory deficits.
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General info

Function

Actin-dependent motor protein with a protein kinase activity, playing an essential role in hearing (PubMed : 12032315, PubMed : 29880844, PubMed : 34788109). Probably plays also a role in vision. Required for normal cochlear hair bundle development and hearing. Plays an important role in the early steps of cochlear hair bundle morphogenesis. Influences the number and lengths of stereocilia to be produced and limits the growth of microvilli within the forming auditory hair bundles thereby contributing to the architecture of the hair bundle, including its staircase pattern. Involved in the elongation of actin in stereocilia tips by transporting the actin regulatory factor ESPN to the plus ends of actin filaments (PubMed : 29880844, PubMed : 34788109).

Sequence similarities

In the C-terminal section; belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.. In the N-terminal section; belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family.

Subcellular localisation

Cytoskeleton

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Product protocols

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Target data

Actin-dependent motor protein with a protein kinase activity, playing an essential role in hearing (PubMed : 12032315, PubMed : 29880844, PubMed : 34788109). Probably plays also a role in vision. Required for normal cochlear hair bundle development and hearing. Plays an important role in the early steps of cochlear hair bundle morphogenesis. Influences the number and lengths of stereocilia to be produced and limits the growth of microvilli within the forming auditory hair bundles thereby contributing to the architecture of the hair bundle, including its staircase pattern. Involved in the elongation of actin in stereocilia tips by transporting the actin regulatory factor ESPN to the plus ends of actin filaments (PubMed : 29880844, PubMed : 34788109).
See full target information Myosin-IIIa
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