Recombinant Human Myoferlin protein is a Human Fragment protein, in the 655 to 754 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
D A V N T L L A M A E R L Q T N I E A L K S G I Q G K I P A N Q L A E L W L K L I D E V I E D T R Y T L P L T E G K A N V T V L D T Q I R K L R S R S L S Q I H E A A V R M R S E A T D V K S T L A E I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR (By similarity).
Alternative names
FER1L3, KIAA1207, MYOF, Myoferlin, Fer-1-like protein 3
Recommended products
Recombinant Human Myoferlin protein is a Human Fragment protein, in the 655 to 754 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q9NZM1-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- D A V N T L L A M A E R L Q T N I E A L K S G I Q G K I P A N Q L A E L W L K L I D E V I E D T R Y T L P L T E G K A N V T V L D T Q I R K L R S R S L S Q I H E A A V R M R S E A T D V K S T L A E I
- Accession
- Q9NZM1
- Protein length
- Fragment
- Amino acids
- 655 to 754
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR (By similarity).
- Sequence similarities
Belongs to the ferlin family.
- Subcellular localisation
- Nucleus membrane
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Myoferlin alternatively known as MYOF is a member of the ferlin family proteins and plays a mechanical role in membrane repair and fusion. It has a molecular mass of approximately 230 kDa. Myoferlin expresses mainly in muscle and endothelial tissues. Its function is critical during muscle development and regeneration as it participates in the fusion of myoblasts into mature myotubes. Also Myoferlin is involved in the repair of damaged sarcolemmal membranes in muscle cells.
- Biological function summary
The protein supports membrane dynamics and stabilization. Myoferlin is not part of a large complex but interacts with other proteins at the cellular membrane. It regulates cellular processes such as proliferation differentiation and endocytosis. In muscle cells it assists in the organization and function of the cytoskeleton maintaining proper cellular architecture and signaling.
- Pathways
Myoferlin engages in critical roles in pathways related to cell membrane repair and trafficking. It associates with pathways like the myogenesis pathway and integrin signaling. In these pathways it interacts with other proteins such as dysferlin and caveolin-3 which facilitate membrane repair and receptor localization respectively. Myoferlin ensures efficient cellular response to membrane damage maintaining tissue integrity.
- Associated diseases and disorders
Myoferlin is connected to muscular dystrophies and cancer. It correlates with the progression of muscular disorder due to its deficiency in membrane repair mechanisms. Additionally in cancer its expression levels relate to tumor invasion and metastasis involving interactions with proteins like E-cadherin and MMP-9. Investigating Myoferlin's role can aid in understanding its potential as a therapeutic target in these diseases.
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1 product image
SDS-PAGE - Recombinant Human Myoferlin protein (ab161989)
ab161989 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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