Recombinant Human Myosin Light Chain 2 protein is a Human Full Length protein, in the 1 to 166 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, WB.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M A P K K A K K R A G G A N S N V F S M F E Q T Q I Q E F K E A F T I M D Q N R D G F I D K N D L R D T F A A L G R V N V K N E E I D E M I K E A P G P I N F T V F L T M F G E K L K G A D P E E T I L N A F K V F D P E G K G V L K A D Y V R E M L T T Q A E R F S K E E V D Q M F A A F P P D V T G N L D Y K N L V H I I T H G E E K D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab79185 can be used as a WB positive control in conjunction with Anti-Myosin Light Chain 2 antibody [EPR3741] ab92721. |
Target data
Function
Contractile protein that plays a role in heart development and function (PubMed:23365102, PubMed:32453731). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (By similarity).
Alternative names
MLC2, MYL2, MLC-2, MLC-2v, Cardiac myosin light chain 2, Ventricular myosin light chain 2, MLC-2s/v
Recommended products
Recombinant Human Myosin Light Chain 2 protein is a Human Full Length protein, in the 1 to 166 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- P10916-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 40% Glycerol (glycerin, glycerine), 0.242% Tris, 0.0555% Calcium chloride
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M A P K K A K K R A G G A N S N V F S M F E Q T Q I Q E F K E A F T I M D Q N R D G F I D K N D L R D T F A A L G R V N V K N E E I D E M I K E A P G P I N F T V F L T M F G E K L K G A D P E E T I L N A F K V F D P E G K G V L K A D Y V R E M L T T Q A E R F S K E E V D Q M F A A F P P D V T G N L D Y K N L V H I I T H G E E K D
- Accession
- P10916
- Protein length
- Full Length
- Predicted molecular weight
- 20.9 kDa
- Amino acids
- 1 to 166
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab79185 is purified using conventional chromatography techniques.
General info
- Function
Contractile protein that plays a role in heart development and function (PubMed:23365102, PubMed:32453731). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (By similarity).
- Post-translational modifications
N-terminus is methylated by METTL11A/NTM1.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Myosin Light Chain 2 (MLC2) also known as ventricular myosin regulatory light chain (MLC2v) or smooth muscle myosin light chain is a critical component of the myosin protein complex. It is a low-molecular-weight protein with a mass typically around 20 kDa. Myosin Light Chain 2 plays a mechanical role in muscle contraction where it interacts with myosin heavy chain and actin filaments. This interaction modulates muscle contraction in both cardiac and smooth muscle tissues. MLC2 is ubiquitously expressed in muscles but exhibits specific expression patterns in cardiac and smooth muscles.
- Biological function summary
Myosin Light Chain 2 plays a fundamental role in muscle function by regulating myofilament sensitivity to calcium ions. It functions as part of the myosin complex which is essential for actin-myosin interactions required for muscle contraction. Phosphorylation of MLC2 alters the actomyosin activity affecting muscle contraction strength and speed. This regulation contributes significantly to muscle tone contractility and overall heart function.
- Pathways
Myosin Light Chain 2 is a pivotal player in the calcium signaling and smooth muscle contraction pathways. Within these pathways phosphorylation leads to myosin cross-bridge cycling with actin facilitated by proteins like calmodulin and myosin light chain kinase (MLCK). This biochemical interaction not only underpins smooth muscle function but also plays roles in other processes like cell motility and division emphasizing MLC2's versatility in cellular mechanics.
- Associated diseases and disorders
Aberrations in Myosin Light Chain 2 function or expression implicate it in cardiac hypertrophy and certain smooth muscle dysfunctions such as hypertension. Changes in MLC2 phosphorylation state can directly affect cardiac contractility worsening conditions like hypertrophic cardiomyopathy. It also relates closely with other proteins such as MLCK and calmodulin which are involved in these disease pathways highlighting a complex network of regulatory mechanisms that when disrupted can lead to severe health issues.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
SDS-PAGE - Recombinant Human Myosin Light Chain 2 protein (ab79185)
15% SDS-PAGE showing ab79185 at approximately 21kDa (3μg).
Western blot - Recombinant Human Myosin Light Chain 2 protein (ab79185)
All lanes: Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (Anti-Myosin Light Chain 2 antibody [EPR3741] ab92721) at 1/10000 dilution
Lane 1: Western blot - Recombinant Human Myosin Light Chain 2 protein (ab79185) at 0.1 µg
Lane 2: Western blot - Recombinant Human Myosin Light Chain 2 protein (ab79185) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Exposure time: 4min
Western blot - Recombinant Human Myosin Light Chain 2 protein (ab79185)
All lanes: Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (Anti-Myosin Light Chain 2 antibody [EPR3741] ab92721) at 1/10000 dilution
Lane 1: Western blot - Recombinant Human Myosin Light Chain 2 protein (ab79185) at 0.1 µg
Lane 2: Western blot - Recombinant Human Myosin Light Chain 2 protein (ab79185) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Exposure time: 8min
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com