Recombinant Human Myosin light chain 3 protein is a Human Full Length protein, in the 1 to 195 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M A P K K P E P K K D D A K A A P K A A P A P A P P P E P E R P K E V E F D A S K I K I E F T P E Q I E E F K E A F M L F D R T P K C E M K I T Y G Q C G D V L R A L G Q N P T Q A E V L R V L G K P R Q E E L N T K M M D F E T F L P M L Q H I S K N K D T G T Y E D F V E G L R V F D K E G N G T V M G A E L R H V L A T L G E R L T E D E V E K L M A G Q E D S N G C I N Y E A F V K H I M S S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Regulatory light chain of myosin. Does not bind calcium.
Alternative names
Myosin light chain 3, Cardiac myosin light chain 1, Ventricular myosin alkali light chain, Ventricular myosin light chain 1, Ventricular/slow twitch myosin alkali light chain, CMLC1, MLC1SB, VLCl, MLC-lV/sb, MYL3
Recommended products
Recombinant Human Myosin light chain 3 protein is a Human Full Length protein, in the 1 to 195 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- P08590-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A P K K P E P K K D D A K A A P K A A P A P A P P P E P E R P K E V E F D A S K I K I E F T P E Q I E E F K E A F M L F D R T P K C E M K I T Y G Q C G D V L R A L G Q N P T Q A E V L R V L G K P R Q E E L N T K M M D F E T F L P M L Q H I S K N K D T G T Y E D F V E G L R V F D K E G N G T V M G A E L R H V L A T L G E R L T E D E V E K L M A G Q E D S N G C I N Y E A F V K H I M S S
- Accession
- P08590
- Protein length
- Full Length
- Amino acids
- 1 to 195
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Regulatory light chain of myosin. Does not bind calcium.
- Post-translational modifications
The N-terminus is blocked.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Myosin light chain 3 (MLC3) also known as myosin regulatory light chain 3 is a part of the myosin protein complex. It plays a major role in muscle contraction by regulating the ATPase activity of myosin heads. MLC3 is encoded by the MYL3 gene and has a molecular mass of approximately 18kDa. It is expressed mainly in cardiac and skeletal muscle tissues. By interacting with myosin heavy chains MLC3 contributes to the force-generating capacity of muscles.
- Biological function summary
MLC3 functions as an essential component of the myosin light chain complex. This complex regulates contractile activity in muscle cells by modulating the interaction between actin and myosin chains. MLC3 activates the mechanical movement of myosin chains impacting cardiac and skeletal muscle contraction. When activated it affects the speed and strength of muscle contraction and it also has a role in muscle differentiation and development.
- Pathways
MLC3 is an integral part of the muscle contraction pathway. It interacts with proteins such as tropomyosin and troponin which are important for the regulation of muscle contraction. Additionally MLC3 participates in the calcium signaling pathway which is pivotal for contraction processes in muscles. This interaction helps to control the cytosolic calcium concentration a critical factor in muscle contraction dynamics.
- Associated diseases and disorders
MLC3 has connections to cardiomyopathy and hypertrophic conditions. Mutations or dysregulation of MYL3 can lead to cardiac hypertrophy a disease where the heart muscle thickens abnormally. MLC3's interaction with troponin and other myosin chains can exacerbate cardiac conditions when these pathways are disrupted. Studies have linked deficiencies in MLC3 to improper muscle development which may underline some muscle myopathies.
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1 product image
SDS-PAGE - Recombinant Human Myosin light chain 3 protein (ab158937)
ab158937 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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