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AB132100

Recombinant Human N-ethylmaleimide-sensitive fusion protein

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Recombinant Human N-ethylmaleimide-sensitive fusion protein is a Human Full Length protein, in the 1 to 744 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

Vesicle-fusing ATPase, N-ethylmaleimide-sensitive fusion protein, Vesicular-fusion protein NSF, NEM-sensitive fusion protein, NSF

1 Images
SDS-PAGE - Recombinant Human N-ethylmaleimide-sensitive fusion protein (AB132100)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human N-ethylmaleimide-sensitive fusion protein (AB132100)

12.5% SDS-PAGE stained with Coomassie Blue showing ab132100

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

P46459

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(Recombinant protein).</p>" } } }
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Product details

This product was previously labelled as NSF.
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Sequence info

[{"sequence":"MAGRSMQAARCPTDELSLTNCAVVNEKDFQSGQHVIVRTSPNHRYTFTLKTHPSVVPGSIAFSLPQRKWAGLSIGQEIEVSLYTFDKAKQCIGTMTIEIDFLQKKSIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSFNEKLFGLLVKDIEAMDPSILKGEPATGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFSTTIHVPNIATGEQLLEALELLGNFKDKERTTIAQQVKGKKVWIGIKKLLMLIEMSLQMDPEYRVRKFLALLREEGASPLDFD","proteinLength":"Full Length","predictedMolecularWeight":"109 kDa","actualMolecularWeight":null,"aminoAcidEnd":744,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P46459","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N-ethylmaleimide-sensitive fusion protein (NSF) is an important component in the cellular machinery. It unwinds homo-oligomer complexes enabling membrane fusion in cells. NSF also goes by the name of NEM-sensitive factor. It has a mass of about 82 kDa. It is expressed in most tissues where membrane fusion processes occur such as neurons liver and other secretory cells.
Biological function summary

NSF plays a central role in ensuring proper vesicular transport. It is part of the SNARE complex which facilitates the fusion of vesicles with their target membranes. Interaction with small regulatory proteins like SNAPs (Soluble NSF Attachment Proteins) ensures NSF activity in recycling SNARE proteins after vesicle fusion. This process is critical for neurotransmitter release in neuronal synapses and for enzymatic secretion in glandular tissues.

Pathways

NSF contributes significantly to intracellular vesicle trafficking and neurotransmitter release pathways. In these pathways proteins like VAMP synaptobrevin and SNAP-25 interact with NSF to maintain the continuity of vesicle-mediated transport. NSF activity modulates SNARE-mediated vesicle fusion thereby allowing efficient cargo transfer between cellular compartments.

NSF has connections to neurological disorders and certain protein trafficking diseases. Mutations in NSF or dysfunction of its SNARE partners can link to neurodegenerative diseases like Alzheimer’s disease. Proteins like the amyloid-beta precursor protein can also interact aberrantly with NSF pathway components exacerbating disease states. Additionally improper NSF function may relate to cystic fibrosis due to issues in vesicular transport affecting CFTR protein localization.
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Specifications

Form

Liquid

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General info

Function

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity).

Sequence similarities

Belongs to the AAA ATPase family.

Post-translational modifications

Phosphorylation at Ser-569 interferes with homohexamerization.

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Product protocols

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Target data

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity).
See full target information NSF
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Complementary Products

Biochemicals

AB142180

MMP-9 inhibitor

  • 0
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Primary Antibodies

AB126202

Anti-N-ethylmaleimide-sensitive fusion protein antibody

Western blot - Anti-N-ethylmaleimide-sensitive fusion protein antibody (AB126202)

  • 0
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Assay Kits

AB112146

MMP Activity Assay Kit (Fluorometric - Green)

Functional Studies - MMP Activity Assay Kit (Fluorometric - Green) (AB112146)

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Assay Kits

AB139446

MMP2 Inhibitor Screening Assay Kit (Colorimetric)

Functional Studies - MMP2 Inhibitor Screening Assay Kit (Colorimetric) (AB139446)

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