Recombinant Human n-Myc/MYCN protein (His tag) is a Human Full Length protein, in the 1 to 464 aa range, expressed in Yeast, with >90% purity and suitable for SDS-PAGE.
Images
Publications
Filter by
- Molecular cell 82:140-158.e122021MYC assembles and stimulates topoisomerases 1 and 2 in a "topoisome".Applications:Unspecified applicationReactive species:Unspecified reactive speciesSubhendu K Das,Vladislav Kuzin,Donald P Cameron,Suzanne Sanford,Rajiv Kumar Jha,Zuqin Nie,Marta Trullols Rosello,Ronald Holewinski,Thorkell Andresson,Jan Wisniewski,Toyoaki Natsume,David H Price,Brian A Lewis,Fedor Kouzine,David Levens,Laura BaranelloPubMed 34890565
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Yeast
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M P S C S T S T M P G M I C K N P D L E F D S L Q P C F Y P D E D D F Y F G G P D S T P P G E D I W K K F E L L P T P P L S P S R G F A E H S S E P P S W V T E M L L E N E L W G S P A E E D A F G L G G L G G L T P N P V I L Q D C M W S G F S A R E K L E R A V S E K L Q H G R G P P T A G S T A Q S P G A G A A S P A G R G H G G A A G A G R A G A A L P A E L A H P A A E C V D P A V V F P F P V N K R E P A P V P A A P A S A P A A G P A V A S G A G I A A P A G A P G V A P P R P G G R Q T S G G D H K A L S T S G E D T L S D S D D E D D E E E D E E E E I D V V T V E K R R S S S N T K A V T T F T I T V R P K N A A L G P G R A Q S S E L I L K R C L P I H Q Q H N Y A A P S P Y V E S E D A P P Q K K I K S E A S P R P L K S V I P P K A K S L S P R N S D S E D S E R R R N H N I L E R Q R R N D L R S S F L T L R D H V P E L V K N E K A A K V V I L K K A T E Y V H S L Q A E E H Q L L L E K E K L Q A R Q Q Q L L K K I E H A R T C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Positively regulates the transcription of MYCNOS in neuroblastoma cells.
Alternative names
BHLHE37, NMYC, MYCN, N-myc proto-oncogene protein, Class E basic helix-loop-helix protein 37, bHLHe37
Recommended products
Recombinant Human n-Myc/MYCN protein (His tag) is a Human Full Length protein, in the 1 to 464 aa range, expressed in Yeast, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Yeast
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P04198-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- M P S C S T S T M P G M I C K N P D L E F D S L Q P C F Y P D E D D F Y F G G P D S T P P G E D I W K K F E L L P T P P L S P S R G F A E H S S E P P S W V T E M L L E N E L W G S P A E E D A F G L G G L G G L T P N P V I L Q D C M W S G F S A R E K L E R A V S E K L Q H G R G P P T A G S T A Q S P G A G A A S P A G R G H G G A A G A G R A G A A L P A E L A H P A A E C V D P A V V F P F P V N K R E P A P V P A A P A S A P A A G P A V A S G A G I A A P A G A P G V A P P R P G G R Q T S G G D H K A L S T S G E D T L S D S D D E D D E E E D E E E E I D V V T V E K R R S S S N T K A V T T F T I T V R P K N A A L G P G R A Q S S E L I L K R C L P I H Q Q H N Y A A P S P Y V E S E D A P P Q K K I K S E A S P R P L K S V I P P K A K S L S P R N S D S E D S E R R R N H N I L E R Q R R N D L R S S F L T L R D H V P E L V K N E K A A K V V I L K K A T E Y V H S L Q A E E H Q L L L E K E K L Q A R Q Q Q L L K K I E H A R T C
- Accession
- P04198
- Protein length
- Full Length
- Predicted molecular weight
- 51.6 kDa
- Amino acids
- 1 to 464
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Positively regulates the transcription of MYCNOS in neuroblastoma cells.
- Post-translational modifications
Phosphorylated by GSK3-beta which may promote its degradation (PubMed:24391509). Phosphorylated by AURKA (PubMed:27837025).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as n-Myc
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
N-Myc also known as MYCN is a transcription factor encoded by the MYCN gene. It plays an important role in cell proliferation and differentiation. The n-Myc protein is part of the Myc family of transcription factors including c-Myc and c-Myb. n-Myc has a molecular weight of approximately 64 kDa. This protein expresses predominantly in developing tissues particularly in the brain but its expression is seen in other tissues during embryogenesis as well. This characteristic makes it important in early development and organ formation.
- Biological function summary
N-Myc/MYCN functions in gene regulation by binding to E-box sequences in DNA facilitating transcription of genes essential for cellular growth and metabolism. n-Myc often forms a heterodimer with the MAX protein which enhances its DNA-binding capacity and transcriptional activity. This activity influences processes like apoptosis cell differentiation and cell cycle progression. The ncm mouse model a genetically engineered mouse with MYCN overexpression demonstrates the effects of deregulated n-Myc expression.
- Pathways
MYCN significantly impacts the p53 signaling pathway and the Wnt signaling pathway. In the p53 signaling pathway n-Myc interacts with p53 and MDM2 regulating cell cycle checkpoints and apoptosis. In the Wnt pathway MYCN interplays with molecules like β-catenin to influence cell division and differentiation. These interactions highlight the role of n-Myc in maintaining cellular homeostasis and response to external signals that affect growth and division.
- Associated diseases and disorders
N-Myc is closely linked to neuroblastoma and medulloblastoma aggressive forms of pediatric cancers. MYCN amplification often indicates a poor prognosis in neuroblastoma playing a role in tumor progression and resistance to therapy. In medulloblastoma overexpression of n-Myc partners with proteins like Aurora A kinase to promote oncogenesis. Targeting these protein interactions offers potential therapeutic strategies for these MYCN-related cancers.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human n-Myc/MYCN protein (His tag) (ab241520)
ab241520 analyzed by (Tris-Glycine gel) discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com