Recombinant human N6AMT2 protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Protein N-lysine methyltransferase that selectively catalyzes the trimethylation of EEF1A at 'Lys-79'.
Alternative names
N6AMT2, EEF1AKMT1, EEF1A lysine methyltransferase 1, N(6)-adenine-specific DNA methyltransferase 2, Protein-lysine N-methyltransferase N6AMT2, eEF1A-KMT
Recommended products
Recombinant human N6AMT2 protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab268808 was 110 pmol/min/mg in a methyltransferase assay using histone H4 peptide (1-21) as substrate.
- Accession
- Q8WVE0-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
Sequence info
Amino acid sequence
- Accession
- Q8WVE0
- Protein length
- Full Length
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Protein N-lysine methyltransferase that selectively catalyzes the trimethylation of EEF1A at 'Lys-79'.
- Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. EFM5 family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
N6AMT2 also known as N-6 adenine-specific DNA methyltransferase 2 is a protein responsible for methylating adenine residues in DNA. This post-translational modification involves the transfer of a methyl group to specific DNA sequences affecting gene expression and genomic stability. N6AMT2 weighs approximately 50 kDa and expresses widely in various human tissues including the liver brain and kidneys.
- Biological function summary
N6AMT2 influences chromatin structure and gene regulation through its methyltransferase activity making it an important player in epigenetic regulation. It does not act in isolation but associates with other methyltransferases and chromatin-modifying complexes enhancing its function in modifying gene expression patterns. These associations allow N6AMT2 to regulate the accessibility of DNA to transcription factors and other DNA-binding proteins.
- Pathways
N6AMT2 participates in the DNA methylation pathway integral for controlling gene expression and maintaining DNA integrity. It works closely with other DNA methyltransferases like DNMT1 to ensure the proper methylation marks are set and maintained during DNA replication. Furthermore N6AMT2 engages in the histone modification pathway where it collaborates with histone methyltransferases to coordinate chromatin remodeling and transcriptional regulation.
- Associated diseases and disorders
The aberrant activity of N6AMT2 associates with certain cancers such as hepatocellular carcinoma and glioblastoma. It influences tumorigenesis through its role in DNA methylation and chromatin remodeling which can lead to altered gene expression profiles. N6AMT2 has direct interactions with oncogenic pathways and protein partners like EZH2 a histone-lysine N-methyltransferase highlighting its importance in cancer progression and offering a potential target for therapeutic intervention.
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2 product images
Functional Studies - Recombinant human N6AMT2 protein (Active) (ab268808)
The specific activity of ab268808 was 110 pmol/min/mg in a methyltransferase assay using histone H4 peptide (1-21) as substrate.
SDS-PAGE - Recombinant human N6AMT2 protein (Active) (ab268808)
SDS-PAGE analysis of ab268808.
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